苏云金芽胞杆菌G033A对新发南美番茄潜叶蛾的室内毒力及田间防效

doi:10.16409/j.cnki.2095-039x.2020.02.003

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (2): 175-183.DOI: 10.16409/j.cnki.2095-039x.2020.02.003

苏云金芽胞杆菌G033A对新发南美番茄潜叶蛾的室内毒力及田间防效

张桂芬^1,2, 张毅波^1,2, 张杰¹, 刘万学^1,2, 王玉生¹, 万方浩^1,2, 束长龙¹, 刘慧³, 王福莲⁴, 赵林^1,4, 李庆红⁵, 王树明⁶, 蒋家强⁷

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业农村部作物有害生物综合治理重点实验室, 北京 100193;
2. 农业农村部外来入侵生物预防与控制研究中心, 北京 100193;
3. 全国农业技术推广服务中心, 北京 100026;
4. 长江大学, 荆州 434100;
5. 云南省植保植检站, 昆明 650034;
6. 云南省玉溪市植保植检站, 玉溪 653100;
7. 玉溪阳瑞新型农业发展有限公司, 玉溪 653100

收稿日期:2019-09-13 出版日期:2020-04-08 发布日期:2020-04-16
通讯作者: 张桂芬
作者简介:张桂芬,博士,研究员,E-mail:zhangguifen@caas.cn;张毅波,副研究员,E-mail:zhangyibo@caas.cn
基金资助:
国家重点研发计划（2017YFC1200600，2016YFC1201200）；中国农业科学院科技创新工程项目（caascx-2017-2022-IAS）

Laboratory Toxicity and Field Control Efficacy of Biopesticide Bacillus thuringiensis G033A on the South American Tomato Leafminer Tuta absoluta (Meyrick), a New Invasive Alien Species in China

ZHANG Guifen^1,2, ZHANG Yibo^1,2, ZHANG Jie¹, LIU Wanxue^1,2, WANG Yusheng¹, WAN Fangfao^1,2, SHU Changlong¹, LIU Hui³, WANG Fulian⁴, ZHAO Lin^1,4, LI Qinghong⁵, WANG Shuming⁶, JIANG Jiaqiang⁷

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop, Ministry of Agriculture and Rural Affairs of the People's Republic of China/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Center for Management of Invasive Alien Species, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China;
3. The National Agro-Tech Extension and Service Center, Beijing 100026, China;
4. Yangtze University, Jingzhou 434100, China;
5. Yunnan Plant Protection and Quarantine Station, Kunming 650034, China;
6. Yuxi Plant Protection and Quarantine Station, Yuxi 653100, China;
7. Yuxi Yangrui Agricultural Development Co., Ltd, Yuxi 653100, China

Received:2019-09-13 Online:2020-04-08 Published:2020-04-16

摘要/Abstract

摘要： 南美番茄潜叶蛾Tuta absoluta（Meyrick）是新近传入我国的一种世界毁灭性番茄害虫，对我国番茄产业的潜在威胁巨大。为有效控制南美番茄潜叶蛾，测定了新型苏云金芽胞杆菌基因工程菌G033A（Bt G033A）对该昆虫的室内毒力及田间防效。结果表明，在室内条件下，以32000 IU/mg Bt G033A WP处理过的番茄叶片饲喂南美番茄潜叶蛾幼虫，其室内毒力效果由高至底依次为4龄幼虫，3龄幼虫，1～2龄幼虫，致死中浓度LC₅₀分别为14.63、15.59和23.17 g/L；浓度在10 g/L及以上时，Bt G033A对南美番茄潜叶蛾各龄幼虫的毒力均较高，处理后96 h（第4 d）校正死亡率均超过90%。在田间条件下，以Bt G033A WP 100倍液（10 g/L）喷雾防治低龄幼虫即有较好的防效，药后第5 d对1龄和2龄幼虫的校正死亡率分别为89.1%和98.0%，药后第7 d对1龄和2龄幼虫的校正死亡率均为100%；并且，与常用的生物杀虫药剂鱼藤酮的防效相当。研究结果对有机蔬菜生产基地新发南美番茄潜叶蛾的有效控制及其综合防控方案制定，具有重要指导意义。

关键词: 南美番茄潜叶蛾, 苏云金芽胞杆菌, 室内毒力, 田间防效

Abstract: The South American tomato leafminer Tuta absoluta (Meyrick), a world destructive tomato insect pest, is a new invasive alien species in China and has posed a potential threat to the Chinese tomato industry. To control T. absoluta effectively, the laboratory toxicity and field control efficacy of Bacillus thuringiensis G033A (Bt G033A, effective component, 32000 IU/mg), a newly developed bivalent genetically engineered bacteria, were evaluated using leaf dipping and field spraying methods, respectively. Laboratory toxicity was the highest on the 4^th instar larvae of T. absoluta, decreased in the 3^rd instars and further decreased in the 1^stto 2^nd instars, as indicated by LC₅₀ of 14.63, 15.59 and 23.17 g/L, respectively. Bt G033A had high control efficacy on T. absoluta larvae when the concentration of Bt G033A was 10 g/L or above, with a corrected mortality of over 90% at 96 h post treatment. On tomato plants grown in greenhouses, 100-fold diluents (10 g/L) of Bt G033A WP had high control efficacy on young larvae of T. absoluta. The corrected mortalities to the 1^st and 2^nd instars were 89.1% and 98.0%, respectively, on the fifth day after treatment and reached 100% on the seventh day after treatment. The insecticide activity of Bt G033A is comparable to that of 5% the Rotenone. The results can benefit the management of the South American tomato leafminer in organic vegetable production bases.

Key words: Tuta absoluta, Bacillus thuringiensis, indoor toxicity, field control efficacy

中图分类号:

S476.1

张桂芬, 张毅波, 张杰, 刘万学, 王玉生, 万方浩, 束长龙, 刘慧, 王福莲, 赵林, 李庆红, 王树明, 蒋家强. 苏云金芽胞杆菌G033A对新发南美番茄潜叶蛾的室内毒力及田间防效[J]. 中国生物防治学报, 2020, 36(2): 175-183.

ZHANG Guifen, ZHANG Yibo, ZHANG Jie, LIU Wanxue, WANG Yusheng, WAN Fangfao, SHU Changlong, LIU Hui, WANG Fulian, ZHAO Lin, LI Qinghong, WANG Shuming, JIANG Jiaqiang. Laboratory Toxicity and Field Control Efficacy of Biopesticide Bacillus thuringiensis G033A on the South American Tomato Leafminer Tuta absoluta (Meyrick), a New Invasive Alien Species in China[J]. Chinese Journal Of Biological Control, 2020, 36(2): 175-183.

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苏云金芽胞杆菌G033A对新发南美番茄潜叶蛾的室内毒力及田间防效

Laboratory Toxicity and Field Control Efficacy of Biopesticide Bacillus thuringiensis G033A on the South American Tomato Leafminer Tuta absoluta (Meyrick), a New Invasive Alien Species in China

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