8种植物精油对嗜卷书虱的杀虫活性及香青兰精油的GC-MS成分分析

doi:10.16409/j.cnki.2095-039x.2020.02.017

摘要/Abstract

摘要： 为筛选新型环保植物源仓储害虫杀虫剂，采用水蒸汽蒸馏法提取8种植物精油，滤纸药膜法、密闭空气熏蒸法测定精油对嗜卷书虱成虫的触杀、熏蒸活性，筛选出高活性植物，并用气相质谱-色谱联用仪（GC-MS）对高活性植物精油进行化学成分的分析和鉴定。结果表明：香青兰、窃衣、啤酒花精油对嗜卷书虱具有较强的触杀活性，LD₅₀分别为157.43、349.34和633.07µ；g/cm²；香青兰、蒌蒿和窃衣精油对嗜卷书虱具有较好的熏蒸活性，LC₅₀分别为0.20、0.70和1.66 mg/L。香青兰精油经GC-MS分析，检测并鉴定到23个化学成分，相对含量占总含量的84.22%，其主要成分为乙酸香叶酯（16.26%）、丁香酚（13.90%）、香叶醛（13.20%）、草蒿脑（7.89%）、丙烯酸-2-乙基己酯（8.55%）以及橙花醛（7.60%）。研究结果表明香青兰精油对嗜卷书虱的生物活性较好，值得进一步研究。

关键词: 嗜卷书虱, 香青兰, 植物精油, 生物活性

Abstract: Eight plant oils were extracted from wild plants usig steaming distillation in screening for new insecticides for storage pests. Essential oils were evaluated for insecticidal activities against Liposcelis bostrychophil using filter-paper film method and fumigation method. Three essential oils (Dracocephalum moldavica, Torilis scabra, and Humulus lupulus) showed strong contact toxicity against L. bostrychophila, with LD₅₀ values of 157.43, 349.34and 633.07 µg/cm², respectively. Three essential oils (D. moldavica, Artemisia selengensis and T. scabra) showed strong fumigant toxicity against the booklouse, with LC₅₀ values of 0.20, 0.70 and 1.66 mg/L, respectively. The essential oil from D. moldavica that exhibited the strongest contact and fumigant toxicity was analyzed by gas chromatography-mass spectrometry (GC-MS) for chemical composition. Twenty three compounds were identified, accounting for 84.22% of the total oil, principallygeranyl acetate (16.26%), eugenol (13.90%), citral (13.20%), 2-Ethylhexyl acrylate (8.55%), estragole (7.89%), and neral (7.60%). The above results indicate that the essential oil of D. moldavica has the potential to be developed as an insecticide for storage pests.

Key words: Liposcelis bostrychophila, Dracocephalum moldavica, essential oil, insecticidal activity

中图分类号:

S435

赵娜娜, 路伟, 古丽米热·艾买提, 傅文佳, 杜书亚. 8种植物精油对嗜卷书虱的杀虫活性及香青兰精油的GC-MS成分分析[J]. 中国生物防治学报, 2020, 36(2): 300-306.

ZHAO Nana, LU Wei, GVLMIRA Amat, FU Wenjia, DU Shuya. Insecticidal Activity of 8 Essential Oils and Analysis of Chemical Composition of Dracocephalum moldavica by GC-MS[J]. Chinese Journal Of Biological Control, 2020, 36(2): 300-306.

参考文献

[1] 姜俊伊, 石天玉, 曹阳, 等. 模拟粮仓机械通风对嗜卷书虱防治效果研究[J]. 中国粮油学报, 2018, 33(9):100-105, 130.
[2] 苏柳, 钱文焱, 国金昱, 等. 植物精油防治害虫及作用机理研究进展[J]. 内蒙古林业科技, 2018, 44(1):61-64.
[3] 董菲, 田玉红, 黄琼, 等. 植物精油对害虫的驱避与毒杀活性的应用研究进展[J]. 农药, 2018, 57(11):781-784, 788.
[4] Lee B H, Choi W, Lee S E, et al. Fumigant toxicity of essential oils and their constituent compounds towards the rice weevil, Sitophilus oryzae (L.)[J]. Crop Protection, 2001, 20:317-320.
[5] Liu C H, Mishra A K, Tan R X, et al. Repellent and insecticidal activities of essential oils from Artemisia princeps and Cinnamomum camphora and their effect on seed germination of wheat and broad bean[J]. Bioresource Technology, 2006, 97(15):1969-1973.
[6] Liang Y, Li J L, Xu S, et al. Evaluation of repellency of some chinese medicinal herbs essential oils against Liposcelis bostrychophila (Psocoptera:Liposcelidae) and Tribolium castaneum (Coleoptera:Tenebrionidae)[J]. Journal of Economic Entomology, 2013, 106(1):513-519.
[7] 侯华民, 张兴. 植物精油对三种鳞翅目害虫的杀虫活性[J]. 植物保护学报, 2002, 29(3):223-228.
[8] 冯长根, 李琼. 香青兰化学成分研究[J]. 中成药, 2006, 28(1):94-98.
[9] 李杰. 民族药用植物香青兰近年研究概况[J]. 中国民族医药杂志, 2015, 21(4):55-62.
[10] 尤努斯江·吐拉洪, 吐尔洪·买买提. 超声提取香青兰中黄酮及其抗氧化活性[J]. 食品科学, 2012, 33(24):72-76.
[11] 杨丽娜, 邢建国, 何承辉, 等. 维药香青兰的化学成分与药理作用评价[J]. 世界临床药物, 2013, 34(4):226-231.
[12] Chu S S, Liu S L, Jiang G H, et al. Composition and toxicity of essential oil of Illicium simonsii Maxim (Illiciaceae) fruit against the maize weevils[J]. Records of Natural Products, 2010, 4(4):205-210.
[13] Sakauma M. Probit analysis of preference data[J]. Applied Entomology and Zoology, 1998, 33:339-347.
[14] Alaei S, Mahna N. Comparison of essential oil composition in Dracocephalum moldavica in greenhouse and field[J]. Journal of Essential Oil Bearing Plants, 2013, 16(3):346-351.
[15] Golparvar A R, Hadipanah A, Gheisari M M, et al. Chemical constituents of essential oil of Dracocephalum moldavica L. and Dracocephalum kotschyi Boiss. from Iran[J]. Acta Agriculturae Slovenica, 2016, 107(1):25-31.
[16] 李雅丽, 邹广平, 郭小强, 等. 蒙药香青兰挥发油类成分的提取及GC-MS分析[J]. 时珍国医国药, 2017(1):51-52.
[17] 谭红胜, 禹荣祥, 叶敏, 等. 维药香青兰中挥发油成分的GC-MS分析[J]. 上海中医药大学学报, 2008, 22(2):55-58.
[18] Liu X C, Cheng J, Zhao N N, et al. Insecticidal activity of essential oil of Cinnamomum cassia, and its main constituent, trans-cinnamaldehyde, against the Booklice, Liposcelis bostrychophila[J]. Tropical Journal of Pharmaceutical Research, 2014, 13(10):1697.
[19] Zhao N N, Zhou L, Liu Z L, et al. Evaluation of toxicities of some common spices essential oils from China against Liposcelis bostrychophila[J]. Food Control, 2012, 26(2):486-490.
[20] Liu X C, Liu Z L. Analysis of the essential oil of Illicium henryi Diels root bark and its insecticidal activity against Liposcelis bostrychophila Badonnel[J]. Journal of Food Protection, 2015, 78(4):772-777.
[21] 张莉娟, 周家欣, 王梦真, 等. 头花杜鹃挥发油的化学成分分析及其对3种仓储害虫的毒杀作用[J]. 植物保护, 2019, 45(3):124-129.
[22] Liu Z L, Zhao N N, Liu C M, et al. Identification of insecticidal constituents of the essential oil of Curcuma wenyujin rhizomes against Liposcelis bostrychophila Badonnel[J]. Molecules, 2012, 17(10):12049-12060.
[23] Liu X C, Chen X B, Liu Z L. Gas chromatography-mass spectrometric analysis and insecticidal activity of essential oil of aerial parts of Mallotus apelta (Lour.) Muell.-Arg. (Euphorbiaceae)[J]. Tropical Journal of Pharmaceutical Research, 2014, 13(9):1515.
[24] Liu X C, Zhou L g, Liu Z L. Identification of insecticidal constituents from the essential oil of Valeriana jatamansi Jones against Liposcelis bostrychophila Badonnel[J]. Journal of Chemistry, 2013(2013):853912.
[25] Liu X C, Li Y L, Wang T J, et al. Chemical composition and insecticidal activity of essential oil of Artemisia frigida Willd (Compositae) against two grain storage insects[J]. Tropical Journal of Pharmaceutical Research, 2014, 13(4):587-592.
[26] Liu X C, Li Y P, Li H Q. Identification of repellent and insecticidal constituents of the essential oil of Artemisia rupestris L. aerial parts against Liposcelis bostrychophila Badonnel[J]. Molecules, 2013, 18(9):10733-10746.
[27] Liu X C, Lu X N, Liu Q Z, et al. Evaluation of insecticidal activity of the essential oil of Allium chinense G. Don and its major constituents against Liposcelis bostrychophila Badonnel[J]. Journal of Asia-Pacific Entomology, 2014, 17(4):853-856.
[28] Liu X C, Yang K, Wang S Y, et al. Composition and insecticidal activity of the essential oil of Pelargonium hortorum flowering aerial parts from China against two grain storage insects[J]. Journal of Medicinal Plant Research, 2013, 7(44):3263-3268.
[29] 马艳玲, 李海贤, 曾荣. 丁香酚对金黄色葡菌球菌抗菌作用的探究[J]. 中国酿造, 2017, 36(8):130-133.
[30] Bhavya M L, Chandu A G S, Sumithra S D. Ocimum tenuiflorum oil, a potential insecticide against rice weevil with anti-acetylcholinesterase activity[J]. Industrial Crops & Products, 2018, 126:434-439.
[31] 梁忠云, 王国聪. 草蒿脑资源及生物活性应用研究进展[J]. 广西林业科学, 2010, 39(1):49-51.
[32] Eliane S T, Pierre M D J, Leopold N T, et al. Antifungal activity of the essential oil of Cymbopogon citratus (Poaceae) against Phaeoramularia angolensis[J]. Journal of Essential Oil Bearing Plants, 2009, 12(2):7.
[33] 张文平, 傅颖媛, 谢小梅. 柠檬醛、肉桂醛抗曲霉菌作用机制研究[J]. 江西医学院学报, 2003(6):10-13.