茶足柄瘤蚜茧蜂脂代谢相关的滞育关联基因差异表达分析

doi:10.16409/j.cnki.2095-039x.2020.03.022

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (4): 632-636.DOI: 10.16409/j.cnki.2095-039x.2020.03.022

• 研究简报 • 上一篇

茶足柄瘤蚜茧蜂脂代谢相关的滞育关联基因差异表达分析

刘敏¹, 刘爱萍¹, 韩海斌¹, 高书晶¹, 徐林波¹, 岳方正², 黄海广³

1. 中国农业科学院草原研究所, 呼和浩特 010000;
2. 国家林业和草原局森林和草原病虫害防治总站, 沈阳 110034;
3. 内蒙古自治区林业科学研究院, 呼和浩特 010000

收稿日期:2019-07-02 出版日期:2020-08-08 发布日期:2020-08-07
通讯作者: 刘爱萍,研究员,E-mail:liuaiping806@sohu.com。
作者简介:刘敏,硕士研究生,E-mail:lm18883338543@163.com
基金资助:
国家重点研发计划（2017YFE0104900，2017YFD02010000）

Differential Expression of Genes Associated with Lipid Metabolism in Diapausing Lysiphlebus testaceipes Cresson

LIU Min¹, LIU Aiping¹, HAN Haibin¹, GAO Shujing¹, XU Linbo¹, YUE Fangzheng², HUANG Haiguang³

1. Institute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010000, China;
2. National Forestry and Grassland Bureau Forest and Grassland Diseases and Insects Pests Control, Shenyang 110034, China;
3. Inner Mongolia Autonomous Region Forestry Science Research Institute, Hohhot 010010, China

Received:2019-07-02 Online:2020-08-08 Published:2020-08-07

摘要/Abstract

摘要： 本文对茶足柄瘤蚜茧蜂滞育组与正常发育组进行转录组测序，筛选差异表达基因，根据功能注释发现这些滞育关联基因主要与碳水化合物代谢、脂质代谢以及信号转导等途径相关。借助KEGG数据库，共筛选出滞育期间401个与脂代谢相关的差异基因，在滞育期间呈现不同程度的上调表达，涉及了脂肪酸生物合成、甘油脂代谢、类固醇生物合成等代谢途径，除与脂质合成相关的脂肪酸合成酶、超长链脂肪酸延伸酶基因上调表达外，与脂质分解相关的酶，如酯酶同样上调表达，可能与提高茶足柄瘤蚜茧蜂免疫力有关；与激素合成相关基因的上调表达，可能抑制茶足柄瘤蚜茧蜂卵巢发育。

关键词: 茶足柄瘤蚜茧蜂, 转录组测序, 滞育关联基因, 脂代谢

Abstract: Lysiphlebus testaceipes Cresson (Hymenoptera:Aphidiidae) is an excellent ectoparasitic wasp of Aphis craccivora Koch. This parasitoid has the potential to suppress populations of A. craccivora. Diapause is a genetic phenomenon of insects adapting to unfavorable environmental conditions. In this study, we employed transcriptome sequencing analyses to screen differentially expressed genes (DEGs) between diapause groups and non-diapause groups. We found that the DEGs were primarily associated with carbohydrate metabolism, lipid metabolism, signal transduction and so on. With the help of KEGG database, a total of 401 DEGs related to lipid metabolism during diapause were screened out. These DEGs, up-regulated to some extent during diapause, are related to fatty acid biosynthesis, glycerol metabolism, steroid biosynthesis and other metabolic pathways. Expression of the genes related to fatty acid synthase and elongase of verylong chain fatty acids was up-regulated, genes of enzymes related to lipid decomposition, such as esterase, were also up-regulated in expression, which may be involved in immunity improvement in L. testaceipes. The up-regulated expression of genes related to hormone synthesis may inhibit the ovarian development.

Key words: Lysiphlebus testaceipes, transcriptomics, diapause-associated gene, lipid metabolism

中图分类号:

S476.3

刘敏, 刘爱萍, 韩海斌, 高书晶, 徐林波, 岳方正, 黄海广. 茶足柄瘤蚜茧蜂脂代谢相关的滞育关联基因差异表达分析[J]. 中国生物防治学报, 2020, 36(4): 632-636.

LIU Min, LIU Aiping, HAN Haibin, GAO Shujing, XU Linbo, YUE Fangzheng, HUANG Haiguang. Differential Expression of Genes Associated with Lipid Metabolism in Diapausing Lysiphlebus testaceipes Cresson[J]. Chinese Journal Of Biological Control, 2020, 36(4): 632-636.

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茶足柄瘤蚜茧蜂脂代谢相关的滞育关联基因差异表达分析

Differential Expression of Genes Associated with Lipid Metabolism in Diapausing Lysiphlebus testaceipes Cresson

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