烟蚜茧蜂滞育关联基因的转录组学分析

doi:10.16409/j.cnki.2095-039x.2017.05.005

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (5): 604-611.DOI: 10.16409/j.cnki.2095-039x.2017.05.005

烟蚜茧蜂滞育关联基因的转录组学分析

安涛, 张洪志, 韩艳华, 陈红印, 张礼生

中国农业科学院植物保护研究所/中美生物防治实验室, 北京 100193

收稿日期:2017-02-07 出版日期:2017-10-08 发布日期:2017-10-11
通讯作者: 张礼生
作者简介:安涛,硕士研究生,E-mail:antaolyzj@163.com;张礼生,研究员,E-mail:zhangleesheng@163.com。
基金资助:
国家自然科学基金（31572062）；国家重点研发计划（SQ2017ZY060059）；中国烟草总公司重大专项（110201601021-LS-01）

Transcriptome Analysis of Diapause-associated Genes of Aphidius gifuensis Ashmaed

AN Tao, ZHANG Hongzhi, HAN Yanhua, CHEN Hongyin, ZHANG Lisheng

Institute of Plant Protection, Chinese Academy of Agricultural Sciences/Sino-American Biological Control Laboratory, USDA-ARS, Beijing 100193, China

Received:2017-02-07 Online:2017-10-08 Published:2017-10-11

摘要/Abstract

摘要： 烟蚜茧蜂Aphidius gifuensis Ashmaed是一种优良的内寄生性天敌昆虫，可有效控制桃蚜等多种蚜虫为害。通过调控温光环境可诱导烟蚜茧蜂进入滞育，既能提升子代烟蚜茧蜂的适应能力和寄生能力，又能显著延长天敌产品的货架期，促进天敌昆虫在害虫生物防治中的应用。本文通过对烟蚜茧蜂的正常发育状态（ND）、滞育状态（D）及滞育解除状态（PD）进行RNA测序，改变并丰富了以往仅对正常和对照组进行分析的常规做法，重点研究只在滞育状态才特异表达、而在正常发育和滞育解除后无显著变化的转录组学波动规律。经分别构建正常发育、滞育、滞育解除样本组，再经RNA提取与纯化，cDNA合成，cDNA文库构建，文库质检合格后进行de novo双端测序，根据对9个样本的测序结果，获取unigene 40477个，获得ND组与D组差异表达基因458个，D组与PD组差异表达基因298个，进一步筛选出D组与ND及PD组显著差异、但ND组与PD组无显著差异的滞育关联基因59个，对后者进行了GO富集、KEGG通路富集等表达分析，根据功能注释发现这些滞育关联基因与自身防御、耐寒性、脂类代谢、表皮黑化、转录调控等途径相关，是影响烟蚜茧蜂滞育进程的重要调控和参与基因。

关键词: 滞育, 烟蚜茧蜂, 滞育关联基因, 转录组学

Abstract: Aphidius gifuensis Ashmead is an excellent solitary endoparasitoid natural enemy, which can control a variety of aphids effectively, especially the aphid Myzus persicae (Sulzer). Diapause plays an important role in the overwintering of A. gifuensis. By simulating the environmental conditions of winter via controlling temperature and photoperiod in the laboratory, we induced the insects to enter diapause for the purposes of enhancing adaptive capacity and parasitic ability of offspring, increasing the production of the biological control agents for pest management. Transcriptome sequencing analyses were employed to compare expression differences between no-diapause (ND), diapause (D) and post-diapause (PD) groups. After RNA extraction and purification, cDNA synthesis, and establishment of cDNA libraries, a total of 40477 unigenes were obtained from 9 samples. We detected 458 differentially expressed genes (DEGs) between ND and D, and 298 DEGs between D and PD. And 59 diapause-associated genes were revealed to be significantly different in D/ND and D/PD, but no significant difference was found in PD/ND. With analysis of GO and KEGG enrichment on diapause-related genes, we found that these genes were primarily associated with cold resistance, autoimmune defense, lipid metabolism, insect cuticle protein and transcriptional regulation, which are the genes regulating the diapause process of A. gifuensis.

Key words: diapause, Aphidius gifuensis, diapause-associated gene, transcriptomics

中图分类号:

S476.3

安涛, 张洪志, 韩艳华, 陈红印, 张礼生. 烟蚜茧蜂滞育关联基因的转录组学分析[J]. 中国生物防治学报, 2017, 33(5): 604-611.

AN Tao, ZHANG Hongzhi, HAN Yanhua, CHEN Hongyin, ZHANG Lisheng. Transcriptome Analysis of Diapause-associated Genes of Aphidius gifuensis Ashmaed[J]. journal1, 2017, 33(5): 604-611.

参考文献

[1] 张礼生, 陈红印, 王孟卿, 等. 寄生蜂的滞育研究进展[J]. 中国生物防治学报, 2014, 30(2):149-164.
[2] Wei J, Li T, Kuang R, et al. Mass rearing of Aphidius gifuensis (Hymenodptera:Aphidiidae) for biological control of Myzus persicae (Homoptera:Aphididae)[J]. Biocontrol Science and Technology, 2003, 13(1):87-97.
[3] Denlinger D L. Why study diapause?[J]. Entomological Research, 2008, 38(1):1-9.
[4] Denlinger D L, Armbruster P A. Mosquito diapause[J]. Entomology, 2014, 59:73-93.
[5] Denlinger D L. Regulation of diapause[J]. Entomology, 2002, 47(47):93-122.
[6] Hahn D A, Denlinger D L. Energetics of insect diapause[J]. Entomology, 2011, 56(56):103-121.
[7] Qi X, Zhang L, Han Y, et al. De novo, transcriptome sequencing and analysis of Coccinella septempunctata L. in non-diapause, diapause and diapause-terminated states to identify diapause-associated genes[J]. BMC Genomics, 2015, 16(1):1-16.
[8] Poelchau M F, Reynolds J A, Denlinger D L, et al. A de novo transcriptome of the Asian tiger mosquito, Aedes albopictus, to identify candidate transcripts for diapause preparation[J]. BMC Genomics, 2011, 12(1):251-254.
[9] 李玉艳, 张礼生, 陈红印, 等. 烟蚜茧蜂滞育诱导的温光周期反应[J]. 应用昆虫学报, 2013, 50(3):718-726.
[10] 张礼生, 陈红印, 李玉艳, 等. 一种调控烟蚜茧蜂滞育的方法[P]. 中国发明专利, CN102640733A, 2012.
[11] Sim C, Kang D S, Kim S, et al. Identification of FOXO targets that generate diverse features of the diapause phenotype in the mosquito Culex pipiens[J]. Proceedings of the National Academy of Sciences, 2015, 112(12):3811-3816.
[12] 梁欣, 陈斌, 乔梁. 昆虫表皮蛋白基因研究进展[J]. 昆虫学报, 2014, 57(9):1084-1093.
[13] Benoit J B, Denlinger D L. Suppression of water loss during adult diapause in the northern house mosquito, Culex pipiens[J]. Journal of Experimental Biology, 2007, 210(2):217-26.
[14] 于奇, 周启升, 赵晓, 等. 神经轴突导向分子Slit的功能及其分子作用机制研究进展[J]. 生理学报, 2012(2):220-230.
[15] 黄凤霞. 烟蚜茧蜂滞育关联蛋白差异表达及其功能研究[D]. 北京:中国农业科学院, 2015.
[16] Lara-Sagahon A V, Kharchenko V, Jose M V. Eco-physiological phases of insect diapause[J]. Journal of Insect Physiology, 2006, 52(2):113-127.
[17] Yocum G D, Joplin K H, Denlinger D L. Upregulation of a 23 kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis[J]. Insect Biochemistry and Molecular Biology, 1998, 28(9):677-682.
[18] Moribe Y, Niimi T, Yamashita O, et al. Samui, a novel cold-inducible gene, encoding a protein with a BAG domain similar to silencer of death domains (SODD/BAG-4), isolated from Bombyx diapause eggs[J]. European Journal of Biochemistry, 2001, 268(12):3432-3442.
[19] Robich R M, Denlinger D L. Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony[J]. Proceedings of the National Academy of Sciences, 2005, 102(44):15912-15917.
[20] 李玉艳. 烟蚜茧蜂滞育诱导的温光周期反应及滞育生理研究[D]. 北京:中国农业科学院, 2011.
[21] 黄凤霞, 蒋莎, 任小云, 等. 烟蚜茧蜂脂代谢相关的滞育相关蛋白差异表达分析[J]. 中国生物防治学报, 2015, 31(6):811-820.
[22] Ashida M, Brey P T. Role of the integument in insect defense:pro-phenol oxidase cascade in the cuticular matrix[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92(23):10698-10702.
[23] 付丹影, 司风玲, 陈斌, 等. 基于转录组的葱蝇表皮蛋白基因家族的鉴定及在非滞育和冬滞育条件下的表达分析[J]. 昆虫学报, 2016, 59(2):172-184.

烟蚜茧蜂滞育关联基因的转录组学分析

Transcriptome Analysis of Diapause-associated Genes of Aphidius gifuensis Ashmaed

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