LmFKBP35基因对飞蝗Toll通路调控作用及对金龟子绿僵菌侵染的影响

doi:10.16409/j.cnki.2095-039x.2026.01.001

中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (5): 1087-1095.DOI: 10.16409/j.cnki.2095-039x.2026.01.001

• 研究论文 • 上一篇

LmFKBP35基因对飞蝗Toll通路调控作用及对金龟子绿僵菌侵染的影响

黄蕾^1,2, 杨文静^1,2, 张靖涵², 王晓玲³, 李健美⁴, 娜木日嘎⁴, 闫冲⁵, 刘文强⁵, 殷怡婷², 涂雄兵², 王广君², 常静¹

1. 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010018;
2. 中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室, 北京 100193;
3. 锡林郭勒盟农牧技术推广中心, 锡林郭勒 026000;
4. 锡林郭勒盟草原工作站, 锡林郭勒 026000;
5. 巴彦淖尔市林业和草原局, 巴彦淖尔 015006

收稿日期:2025-09-11 发布日期:2026-10-21
通讯作者: 王广君,博士,副研究员,E-mail:wangguangjun@caas.cn;常静,博士,副教授,E-mail:changjing10220@sina.com。
作者简介:黄蕾,硕士,E-mail:huanglei9371@163.com。
基金资助:
国家重点研发计划（2023YFE0100100）；现代农业技术产业体系（CARS-34-18）；中央公益性科研机构基础研究基金（S2026XM03，S2025XM04）

The Regulation of LmFKBP35 on Toll Pathway of Locusts migratoria and Its Effect on the Infection of Metarhizium anisopliae

HANG Lei^1,2, YANG Wenjing^1,2, ZHANG Jinghan², WANG Xiaoling³, LI Jianmei⁴, NAMU Riga⁴, YAN Chong⁵, LIU Wenqiang⁵, YIN Yiting², TU Xiongbing², WANG Guangjun², CHANG Jing¹

1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Xilingol League Agricultural and Animal Husbandry Technology Promotion Center, Xilinhot 026000, China;
4. Xilingol League Grassland Workstation, Xilinhot 026000, China;
5. Bayannur Forestry and Grassland Bureau, Bayannur 015006, China

Received:2025-09-11 Published:2026-10-21

摘要/Abstract

摘要： FK506结合蛋白（FKBPs）是一类免疫亲和素，可以与免疫抑制剂FK506结合抑制生物体的免疫反应。LmFKBP35为飞蝗FKBPs蛋白家族中的一员，前期研究发现LmFKBP35与金龟子绿僵菌混合，可以增强绿僵菌对飞蝗的致病力。为进一步明确LmFKBP35基因的功能，本研究克隆了LmFKBP35基因，采用定量PCR技术，对金龟子绿僵菌侵染前后飞蝗中LmFKBP35基因表达量进行了测定；使用RNAi技术，干扰LmFKBP35后，测定了Toll通路中免疫相关基因表达情况以及对绿僵菌侵染的影响。研究结果表明，LmFKBP35基因编码区全长为987 bp，编码328个氨基酸，具有保守FKBP-C超家族结构域和TPR结构域，属于FKBP-C超家族成员；绿僵菌侵染后LmFKBP35基因表达量升高89%，与对照差异显著；对LmFKBP35干扰24 h后，其表达量较对照组显著降低，Toll通路相关基因spätzle、cactus和抗菌肽defensin表达量分别显著升高72%、47%和82%；干扰LmFKBP35后绿僵菌处理飞蝗，飞蝗的存活率与单独绿僵菌处理相比显著提高22%。据上述研究结果推测LmFKBP35基因可抑制飞蝗免疫，促进绿僵菌的侵染。

关键词: 飞蝗, LmFKBP35, 金龟子绿僵菌, 免疫机制

Abstract: FK506-binding proteins (FKBPs) are a kind of immunophilins that bind the immunosuppressant FK506 and inhibit immune responses. LmFKBP35, a member of the FKBPs family in the Locusta migratoria, was previously demonstrated to enhance the pathogenicity of Metarhizium anisopliae when they were mixed together. The function of the LmFKBP35 gene was clarified by cloning the gene and determining the expression of the gene in L. migratoria before and after infection with M. anisopliae by quantitative PCR (qPCR). Furthermore, employing RNA interference (RNAi), we investigated the influence of LmFKBP35 knockdown on the expression of immune-related genes in the Toll pathway and on M. anisopliae pathogenicity. The results showed that LmFKBP35 gene has a 987-bp coding sequence encoding a 328-amino acid protein containing conserved FKBP-C and TPR domains, confirming its membership in the FKBP-C superfamily. LmFKBP35 expression increased significantly by 89% after M. anisopliae infection. Twenty-four hours after RNAi knockdown, LmFKBP35 expression was significantly reduced by 92% compared to controls, while expression of spätzle, cactus, and defensin significantly increased by 72%, 47%, and 82%, respectively. When L. migratoria received simultaneous LmFKBP35 knockdown and M. anisopliae infection (via oral delivery), they exhibited a significantly higher survival rate (45%) compared to the control group (23%), representing a 22% increase. Based on the above results, it is inferred that the LmFKBP35 gene can suppresses the immune responses in L. migratoria.

Key words: Locusta migratoria, LmFKBP35, Metarhizium anisopliae, immune mechanism

中图分类号:

S476

黄蕾, 杨文静, 张靖涵, 王晓玲, 李健美, 娜木日嘎, 闫冲, 刘文强, 殷怡婷, 涂雄兵, 王广君, 常静. LmFKBP35基因对飞蝗Toll通路调控作用及对金龟子绿僵菌侵染的影响[J]. 中国生物防治学报, 2026, 42(5): 1087-1095.

HANG Lei, YANG Wenjing, ZHANG Jinghan, WANG Xiaoling, LI Jianmei, NAMU Riga, YAN Chong, LIU Wenqiang, YIN Yiting, TU Xiongbing, WANG Guangjun, CHANG Jing. The Regulation of LmFKBP35 on Toll Pathway of Locusts migratoria and Its Effect on the Infection of Metarhizium anisopliae[J]. Chinese Journal of Biological Control, 2026, 42(5): 1087-1095.

/ 推荐

参考文献

[1] Wang X, Zhou Y, Guan J, et al. FKBP39 controls the larval stage JH activity and development in Drosophila melanogaster[J]. Insects, 2022, 13(4): 330.
[2] Somarelli J A, Lee S Y, Skolnick J, et al. Structure-based classification of 45 FK506-binding proteins[J]. Proteins-Structure Function and Bioinformatics, 2008, 72(1):197-208.
[3] Liu J, Farmer J D Jr., Lane W S, et al. Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes[J]. Cell, 1991, 66(4): 807-815.
[4] Kang S, Shin D, Mathias D K, et al. Homologs of human dengue-resistance genes, FKBP1B and ATCAY, confer antiviral resistance in Aedes aegypti mosquitoes[J]. Insects, 2019, 10(2):46.
[5] 陈郁蕙,黄金花,冯启理,等.家蚕BmFKBP45基因的表达和功能分析[J].华南师范大学学报(自然科学版), 2016, 48(6):1-12, 143.
[6] 尹仙枫,朱凯辉,王芳,等.绿僵菌与FKBP52联合使用对西花蓟马的毒力[J].植物保护学报, 2024, 51(1):178-185.
[7] 陈建红.东亚飞蝗早期感知蝗绿僵菌的机制研究[D].重庆:重庆大学, 2016.
[8] Zhang N, Feng S, Tian Y, et al. Identification, characterization and spatiotemporal expression analysis of the FKBP family genes in Locusta migratoria[J]. Scientific Reports, 2023, 13(1):1-9.
[9] 田野,李贝贝,崔志刚,等.东亚飞蝗FK506结合蛋白基因克隆及其免疫功能分析[J].植物保护学报, 2021, 48(1):106-114.
[10] 田野,李贝贝,李霜,等. FK506结合蛋白FKBP52对金龟子绿僵菌侵染东亚飞蝗的影响[J].植物保护, 2021, 47(2):37-45.
[11] Zhang N, Feng S, Duan S, et al. LmFKBP24 interacts with LmEaster to inhibit the antifungal immunity of Locusta migratoria[J]. Pesticide Biochemistry and physiology, 2023, 195:105515.
[12] Roy M C, Kim Y. Toll signal pathway activating eicosanoid biosynthesis shares its conserved upstream recognition components in a lepidopteran Spodoptera exigua upon infection by Metarhizium rileyi, an entomopathogenic fungus[J]. Journal of Invertebrate Pathology, 2022, 188:107707.
[13] Weber A N R, Tauszig-Delamasure S, Hoffmann J A, et al. Binding of the Drosophila cytokine Spatzle to Toll is direct and establishes signaling[J]. Nature Immunology, 2003, 4(8):794-800.
[14] Miao Z, Xiong C, Wang Y, et al. Identification of immunity-related genes distinctly regulated by Manduca sexta Spatzle-1/2 and Escherichia coli peptidoglycan[J]. Insect Biochemistry and Molecular Biology, 2024, 168:104108.
[15] Wu H X, Xu Y T, Zafar J, et al. Transcriptomic analysis revealS the impact of the biopesticide Metarhizium anisopliae on the immune systeM of major workers in Solenopsis invicta[J]. Insects, 2023, 14(8):701.
[16] Jiang S, Bao J, Chen Y, et al. Immunological regulation by Toll-1 and Spätzle-4 in larval density-dependent prophylaxis of the oriental armyworm, Mythimna separata[J]. International Journal of Biological Macromolecules, 2024, 264:130778.
[17] Jalovecka M, Malandrin L, Urbanova V, et al. Activation of the tick Toll pathway to control infection of Ixodes ricinus by the apicomplexan parasite Babesia microti[J]. PLoS Pathogens, 2024, 20(12):1012743.
[18] 高延富,李俊俊,余静,等.小菜蛾Cactus基因的克隆及表达模式研究[J].环境昆虫学报, 2018, 40(1):161-172.
[19] Zhang C, He L, Yang H, et al. Effects of microbial infection on key gene expression in the Toll signaling pathway and immune response in Myzus persicae[J]. Pesticide Biochemistry and Physiology, 2025, 214:106611.
[20] Wang M H, Tian Y, Zhang N, et al. Molecular identification and related functional characterization of the FKBP52 gene in immunity of Locusta migratoria manilensis (Orthoptera:Oedipodidae)[J]. Journal of Economic Entomology, 2024, 117(3):1130-1140.
[21] 杨文静.防治飞蝗高效绿僵菌工程菌的构建[D].呼和浩特:内蒙古农业大学, 2024.
[22] 庞继鑫,肖关丽,杜广祖,等.一株罹病草地贪夜蛾成虫莱氏绿僵菌的分离鉴定及其致病力[J].中国生物防治学报, 2023, 39(2):331-339.
[23] Lemaitre B. Landmark-The road to Toll[J]. Nature Reviews Immunology, 2004, 4(7):521-527.
[24] Myllymäki H, Rämet M. JAK/STAT pathway in Drosophila immunity[J]. Scandinavian Journal of Immunology, 2014, 79(6):377-385.
[25] Bang I S. JAK/STAT signaling in insect innate immunity[J]. Entomological Research, 2019, 49(8):339-353.
[26] Hillyer J F. Insect immunology and hematopoiesis[J]. Developmental and Comparative Immunology, 2016, 58:102-118.
[27] Harding M W, Galat A, Uehling D E, et al. A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase[J]. Nature, 1989, 341(6244):758-760.
[28] Siekierka J J, Staruch M J, Hung S H, et al. FK-506, a potent novel immunosuppressive agent, binds to a cytosolic protein which is distinct from the cyclosporin a-binding protein, cyclophilin[J]. Journal of Immunology (Baltimore, Md.:1950), 1989, 143(5):1580-1583.
[29] Fischer G, Bang H, Mech C. Determination of enzymatic catalysis for the cis-trans-isomerization of peptide binding in proline-containing peptides[J]. Biomedica Biochimica Acta, 1984, 43(10):1101-1111.
[30] Gollan P J, Bhave M, Aro E M. The FKBP families of higher plants:exploring the structures and functions of protein interaction specialists[J]. Febs Letters, 2012, 586(20):3539-3547.
[31] van de Hoef D L, Bonner J M, Boulianne G L. FKBP14 is an essential gene that regulates Presenilin protein levels and Notch signaling in Drosophila[J]. Development, 2013, 140(4):810-819.
[32] Barik S. Immunophilins:for the love of proteins[J]. Cellular and Molecular Life Sciences, 2006, 63(24):2889-2900.
[33] Tong M, Jiang Y. FK506-binding proteins and their diverse functions[J]. Current Molecular Pharmacology, 2016, 9(1):48-65.
[34] Ghartey-Kwansah G, Li Z, Feng R, et al. Comparative analysis of FKBP family protein:evaluation, structure, and function in mammals and Drosophila melanogaster[J]. Bmc Developmental Biology, 2018, 18(1):7.
[35] Kolos J M, Voll A M, Bauder M, et al. FKBP ligands-where we are and where to go?[J]. Frontiers in Pharmacology, 2018, 9:1425.
[36] Geisler M, Bailly A. Tete-a-tete:the function of FKBPs in plant development[J]. Trends in Plant Science, 2007, 12(10):465-473.
[37] Lü M, Mohamed A A, Zhang L, et al. A family of CSαβ defensins and defensin-like peptides from the migratory locust, Locusta migratoria, and their expression dynamics during mycosis and nosemosis[J]. PLoS ONE, 2016, 11(8):0161585.
[38] Li J, Xia Y. Host-pathogen interactions between Metarhizium spp. and locusts[J]. Journal of Fungi, 2022, 8(6):602.

LmFKBP35基因对飞蝗Toll通路调控作用及对金龟子绿僵菌侵染的影响

The Regulation of LmFKBP35 on Toll Pathway of Locusts migratoria and Its Effect on the Infection of Metarhizium anisopliae

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	邓璇璇, 何勇, 袁伏才, 方宝华, 匡炜, 张亚, 刘双清. 金龟子绿僵菌与几种杀虫剂混配对二化螟的毒力及田间防效评价[J]. 中国生物防治学报, 2026, 42(1): 198-207.
[2]	张谦, 王燕, 董明, 冯国艺, 林永增, 梁青龙, 王树林, 祁虹. 金龟子绿僵菌与化学农药减量联用对棉花苗蚜的防控效果[J]. 中国生物防治学报, 2023, 39(4): 797-803.
[3]	徐子龙, 张福斌, 武兆辰, 张晶, 张建珍, 赵小明. LmNotch基因在飞蝗翅发育中的功能分析[J]. 中国生物防治学报, 2022, 38(4): 1001-1008.
[4]	于博驰, 张克诚, 施李鸣, 杨淼泠, 吕朝阳, 葛蓓孛. 武夷菌素对活体微生物农药的影响[J]. 中国生物防治学报, 2022, 38(3): 797-802.
[5]	康奎, 蔡尤俊, 龚俊, 张道伟. 金龟子绿僵菌对褐飞虱繁殖力的影响[J]. 中国生物防治学报, 2022, 38(1): 180-187.
[6]	裴松松, 吴轩, 李瑞军, 赵丹, 陆秀君, 郭巍. 对西花蓟马高效金龟子绿僵菌菌株筛选及在花生田间的应用效果[J]. 中国生物防治学报, 2021, 37(4): 732-739.
[7]	刘海洋, 刘龙, 刘新宇, 王敦. 两株杀虫真菌对陕西榆林草原蝗虫的防治效果[J]. 中国生物防治学报, 2021, 37(2): 380-384.
[8]	陈峰, 王长方, 王俊, 陈文乐, 胡进锋, 吴玮, 姚锦爱. 一株金龟子绿僵菌对茶尺蠖的致病力和胞外酶活性[J]. 中国生物防治学报, 2021, 37(1): 124-129.
[9]	彭国雄, 张淑玲, 夏玉先. 金龟子绿僵菌CQMa421农药及应用情况[J]. 中国生物防治学报, 2020, 36(6): 850-857.
[10]	李贝贝, 田野, 杜桂林, 岳方正, 农向群, 刘廷辉, 张泽华, 王广君. 丝氨酸蛋白酶抑制剂Serpin1对绿僵菌侵染飞蝗的影响[J]. 中国生物防治学报, 2020, 36(5): 729-736.
[11]	徐翔, 杨淞杰, 李维强, 衡晓蓉, 尹勇. 绿僵菌油悬浮剂与减量化学农药联用对冬玉米草地贪夜蛾的防控效果[J]. 中国生物防治学报, 2020, 36(4): 530-533.
[12]	李兴佳, 冯晓洁, 农向群, 蔡霓, 王广君, 周淦, 涂雄兵, 张泽华. 花生田施用绿僵菌对土壤微生物群落的影响[J]. 中国生物防治学报, 2019, 35(6): 867-875.
[13]	黄鹏, 陈汉鑫, 姚锦爱, 黄建成, 余德亿. 金龟子绿僵菌对石蒜绵粉蚧的室内毒力与防治效果[J]. 中国生物防治学报, 2019, 35(6): 884-890.
[14]	彭国雄, 张淑玲, 夏玉先. 杀虫真菌对草地贪夜蛾不同虫态的室内活性[J]. 中国生物防治学报, 2019, 35(5): 729-734.
[15]	彭国雄, 张淑玲, 张维, 夏玉先. 杀虫真菌与苏云金芽胞杆菌对草地贪夜蛾的联合室内杀虫活性研究[J]. 中国生物防治学报, 2019, 35(5): 735-740.