Abstract: Antagonistic bacteria T2WF and W10, resistant to rifampicin at 400μg/ml by continuously screening on series concentration of rifampicin media, were stable in antagonism against Fusarium oxysporum f. sp. cubense, pathogen of banana wilt disease, and were identified as Bacillus spp.. The two marked strains could be reisolated from banana seedlings and rhizosphere soil when they were inoculated in the roots by drenching at the concentration of 5×10⁴ cfu/ml. Results showed that strain T2WF and W10 could colonize, survive and transfer in banana seedlings. The population peaks of marked T2WF strain in rhizosphere soil root, corm and pseudostem of banana were at 5, 5, 11 and 3 d after inoculation, with numbers 707, 437, 273 and 117 cfu/mg fresh weight of soil, correspondingly. Population of strain W10 peaked at 5, 3, 5 and 5 d after inoculation with numbers 784, 260, 253 and 110 cfu/mg fresh weight of soil. Both of the strains' population showed the trend "increased at the beginning and decreased aferward" during 1 to 15 d after inoculation. Population of both the strains was higher in roots than in corm and pseudostem of banana seedlings.