Abstract: Several potential fungal bioherbicidal strains of Fusarium stoveri causing the leaves wither, brown or etiolation and stems rot were isolated from the native Alternanthera philoxeroides. The results revealed the temperature for mycelial growth and conidia production was within range of 25-28℃ with optimum at 28℃. Great difference conidial production and pathogenicity existed among the nine isolates Strain 32-6 displayed the best conidial production, and was selected as the potential bioherbicidal candidate based on the mycelial growth rate, conidial production and pathogenicity to Alternanthera philoxeroides. The results of host-range tests of the strain 32-6 indicated that the pathogen didn't infect the 42 tested agricultural crop and weed species.