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野燕麦生防真菌燕麦叶枯菌的分离培养及致病性研究

张宗俭1,2; 李扬汉1   

  1. 1.南京农业大学杂草研究室,南京 210095;2.西北农业大学植物保护系
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1996-11-08 发布日期:1996-11-08

ISOLATION, CULTIVATION AND PATHOGENESIS STUDIES OF A BIOLOGICAL CONTROL AGENT FOR WILD OATS

ZHANG Zong-jian; LI Yang-han   

  1. Weed Research Laboratory, Nanjing Agricultural University, Jiangsu 210095
  • Received:1900-01-01 Revised:1900-01-01 Online:1996-11-08 Published:1996-11-08

摘要: 1993年5月在江苏南京发现自然罹病的野燕麦植株,对其病原真菌进行鉴定、分离,并对其培养特性作了初步研究,结果表明,其病原菌为燕麦叶枯菌,该菌在PDA培养基上产生灰白色中央隆起的粗糙形菌落,不产孢;在营养缺限型的燕麦粒培养基或水琼脂上的小麦秸秆表面可产生有性阶段子囊果和少量分生孢子。菌丝体接种整株幼苗或离体叶片试验表明,该菌只侵染野燕麦而不感染小麦,是一种潜在的防除野燕麦的真菌除草剂菌株。

Abstract: The natural infected wild oat plants were discovered and collected in the
wheat field of Nanjing, Jiangsuprovince. The pathogen was isolated and identified, and the cultural characteristics and infective ability were also studied. The results showed that the pathogen is Drechslera avenaceae (Curtis ex Cooke) Shoem. The colonies of this fungus are grey to black, with coarse, radiating mycelium for ming characteristic large milky white to greyish turfs in PDA medium , and producing no conidiain PDA. Ascomata and some conidia could be observed on the oat seedsmedium or on wheat straw swhich cultured on the 2% agarmedium. The pathogenesis exper iments showed that this fungus only infects wildoats, not wheat. Futher development and research of this fungus as a selection of mycoherbicide for wildoats control are suggested in this paper.

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