球孢白僵菌在玉米苗期的定殖及其对玉米生理生化特性的影响

doi:10.16409/j.cnki.2095-039x.2016.06.007

中国生物防治学报 ›› 2016, Vol. 32 ›› Issue (6): 721-727.DOI: 10.16409/j.cnki.2095-039x.2016.06.007

球孢白僵菌在玉米苗期的定殖及其对玉米生理生化特性的影响

费泓强¹, 隋丽^2,3, 朱慧^3,4, 徐文静², 陈日曌¹, 汪洋洲², 李启云², 王德利³

1. 吉林农业大学农学院, 长春 130118;
2. 吉林省农业科学院植物保护研究所, 公主岭 136100;
3. 东北师范大学生命科学学院, 长春 130024;
4. 植被生态学教育部重点实验室, 长春 130024

收稿日期:2016-04-28 出版日期:2016-12-08 发布日期:2016-12-07
通讯作者: 李启云,男,研究员,E-mail:qyli@cjass.com;王德利,教授,E-mail:wangd@nenu.edu.cn
作者简介:费泓强(1991-),男,硕士研究生,E-mail:455125236@qq.com;隋丽,女,助理研究员,E-mail:suiyaoyi@163.com
基金资助:
吉林省科技厅重大项目（20140203006NY）；吉林省科技厅自然基金（2015010174JC）；植被生态科学教育部重点实验室开放课题（130026537）

Colonization of Beauveria bassiana in Maize Seedlings and Its Effect on Their Physiological-biochemical Characteristics

FEI Hongqiang¹, SUI Li^2,3, ZHU Hui^3,4, XU Wenjing², CHEN Rizhao¹, WANG Yangzhou², LI Qiyun², WANG Deli³

1. College of Agronomy, Jilin Agricultural University, Changchun 130118, China;
2. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;
3. College of Life Sciences, Northeast Normal University, Changchun 130024, China;
4. Key Laboratory of Vegetation Ecology, Ministry of Education, Changchun 130024, China

Received:2016-04-28 Online:2016-12-08 Published:2016-12-07

摘要/Abstract

摘要： 为明确球孢白僵菌D1-5在玉米苗期的定殖规律及其对玉米生长状态和生理生化特性的影响，采用盆栽控制试验，研究了两种浸种时间（12和24 h）对菌株D1-5在玉米苗期不同部位（根、茎、叶）的定殖偏好性的影响，同时研究了几种生长指标对不同浸种时间的响应，分析了抗性相关防御酶活性（出苗14 d）变化情况。结果表明，叶片中菌株D1-5定殖检出率最高，达到40%，茎部其次，定殖检出率为10%，玉米根系未检测出菌株D1-5；各检测时期菌株D1-5处理组均高于对照组，差异显著，其中玉米出苗19 d生长势增长率最高，达到5.34%，出苗21 d叶面积增长率最高，达到13.71%，出苗28 d地上生物量增长率达到27.2%；菌株D1-5处理后叶片中抗性相关防御酶——苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性均呈升高趋势，SOD活性增加最高，达到24.90%。菌株D1-5浸种对玉米苗期的生长发育有一定的促进作用，其中浸种12 h更有利于玉米幼苗生长，玉米相关防御酶的活性变化同球孢白僵菌-玉米之间的互作存在一定的相关性。

关键词: 球孢白僵菌, 玉米, 定殖, 生理生化特性

Abstract: Colonization of Beauveria bassiana in maize seedlings and its effect on their physiological and biochemical characteristics was investigated by examining colonization positions (leaf, stalk and root) under 2 seed soaking durations (12 and 24 h), and measuring plant growth characteristics and activities of some resistance defense enzymes in 14 d old seedlings. Endophytic colonization of B. bassiana in leaves (40%) was greater than in stalks (10%), and no endophytic colonization in roots was found. Significant effects of seed soaking durations on bio-physiological parameters of maize seedlings were detected. Plant height was increased by 5.34% in 19 d old seedlings, and leaf area and aboveground biomass by 13.71% and 27.2% in 21 d and 28 d old seedlings, respectively. Activities of some resistance defense enzymes, including phenylalnine ammonialyase (PAL), polyphenoloxidase (PPO), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), were enhanced, with increase by 24.90% for SOD. Our study suggests that B. bassiana may have positive effects on plant growth, especially for seed soaking treatment for 12 h. In addition, changes in activities of defense enzymes may be dependent on the interactions between B. bassiana and the seedlings.

Key words: Beauveria bassiana, maize, colonization, physiological and biochemical characteristic

中图分类号:

S476.12

费泓强, 隋丽, 朱慧, 徐文静, 陈日曌, 汪洋洲, 李启云, 王德利. 球孢白僵菌在玉米苗期的定殖及其对玉米生理生化特性的影响[J]. 中国生物防治学报, 2016, 32(6): 721-727.

FEI Hongqiang, SUI Li, ZHU Hui, XU Wenjing, CHEN Rizhao, WANG Yangzhou, LI Qiyun, WANG Deli. Colonization of Beauveria bassiana in Maize Seedlings and Its Effect on Their Physiological-biochemical Characteristics[J]. journal1, 2016, 32(6): 721-727.

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球孢白僵菌在玉米苗期的定殖及其对玉米生理生化特性的影响

Colonization of Beauveria bassiana in Maize Seedlings and Its Effect on Their Physiological-biochemical Characteristics

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