转cry1C,cry2A基因水稻对稻田浮游动物群落物种多样性的影响

doi:10.16409/j.cnki.2095-039x.2017.04.004

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (4): 454-462.DOI: 10.16409/j.cnki.2095-039x.2017.04.004

转cry1C,cry2A基因水稻对稻田浮游动物群落物种多样性的影响

陈怡¹, 杨艳¹, 朱昊俊^1,2, 高彦杰¹, 陈秀萍¹, 彭于发¹

1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193;
2. 扬州大学动物科学与技术学院, 扬州 225009

收稿日期:2017-01-21 出版日期:2017-08-08 发布日期:2017-08-11
通讯作者: 陈秀萍,助理研究员,E-mail:xpchen@ippcaas.cn
作者简介:陈怡,硕士研究生,E-mail:sjbhzcy@163.com
基金资助:
国家转基因生物新品种培育科技重大专项（2016ZX08011-001）

Effects of Transgenic Rice with Genes cry1C and cry2A on Species Diversity of Zooplankton Community in Paddy Fields

CHEN Yi¹, YANG Yan¹, ZHU Haojun^1,2, GAO Yanjie¹, CHEN Xiuping¹, PENG Yufa¹

1. The State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2017-01-21 Online:2017-08-08 Published:2017-08-11

摘要/Abstract

摘要： 转Bt基因水稻的非靶标效应一直以来都是人们关注的重点，为了解转Bt基因水稻对稻田浮游动物群落的安全性，本文以转cry1C基因水稻（T1C-19）、转cry2A基因水稻（T2A-1）及其非转基因亲本水稻明恢63（MH63）为材料，以浮游动物的种类和群落多样性为指标，研究并分析了转Bt基因水稻对稻田浮游动物群落物种多样性的影响。结果显示，水稻不同生长时期里稻田中浮游动物种类变化不明显，数量变化明显，Shannon-Wiener物种多样性指数和均匀性指数在拔节期达到最大值。另外由于孕穗期不得不施用农药防治蝗虫，导致孕穗期浮游动物的数量及物种多样性显著降低，并且在使用农药后，多样性指数和均匀性指数随着水稻的生长逐渐下降。但转Bt基因水稻对浮游动物种类和数量及群落物种多样性与对照非转基因水稻相比均无显著性差异。与施用农药对水体带来的负面影响相比，转Bt基因水稻对水体环境无负面影响。

关键词: 转基因水稻, 浮游动物, 物种多样性, 安全评价

Abstract: In the current study, we investigated the potential effects of transgenic Bt rice on the zooplankton community in rice paddy fields. cry1C transgenic rice (T1C-19), cry2A transgenic rice (T2A-1), and their non-transgenic parental rice Minghui63 (MH63) were evaluated for the zooplankton species and community diversity at different growing stages. The numbers of zooplankton fluctuated obviously in rice paddy fields at different growing stages, with the Shannon-Wiener diversity and evenness indexes reaching the highest levels at the elongation stage. During the booting stage, pesticides had to be used to control locust infestation, which caused the numbers of zooplankton, Shannon-Wiener diversity and evenness indexes to decline throughout the rest of the growing stages. However, no significant differences in the diversity and quantity of zooplankton species were found between fields planted with transgenic Bt rice and non-transgenic Bt rice at all growing stages. Our results show that the use of pesticides, but not the introduction of Bt gene, has negative effects on the zooplankton community in rice paddy fields.

Key words: transgenic rice, zooplankton, species diversity, safety evaluation

中图分类号:

S476

陈怡, 杨艳, 朱昊俊, 高彦杰, 陈秀萍, 彭于发. 转cry1C,cry2A基因水稻对稻田浮游动物群落物种多样性的影响[J]. 中国生物防治学报, 2017, 33(4): 454-462.

CHEN Yi, YANG Yan, ZHU Haojun, GAO Yanjie, CHEN Xiuping, PENG Yufa. Effects of Transgenic Rice with Genes cry1C and cry2A on Species Diversity of Zooplankton Community in Paddy Fields[J]. journal1, 2017, 33(4): 454-462.

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转cry1C,cry2A基因水稻对稻田浮游动物群落物种多样性的影响

Effects of Transgenic Rice with Genes cry1C and cry2A on Species Diversity of Zooplankton Community in Paddy Fields

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