亚致死浓度噻虫嗪对意大利蜜蜂工蜂的生存风险分析

doi:10.16409/j.cnki.2095-039x.2017.05.009

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (5): 638-643.DOI: 10.16409/j.cnki.2095-039x.2017.05.009

亚致死浓度噻虫嗪对意大利蜜蜂工蜂的生存风险分析

岳孟¹, 宗伏霖^2,3, 马昌盛¹, 吴杰¹, 罗术东¹

1. 中国农业科学院蜜蜂研究所/农业部授粉昆虫生物学重点实验室, 北京 100093;
2. 农业部农药检定所, 北京 100026;
3. 中国农业大学, 北京 100083

收稿日期:2017-01-25 出版日期:2017-10-08 发布日期:2017-10-11
通讯作者: 罗术东
作者简介:岳孟,硕士研究生,E-mail:yuemengcaas@163.com;罗术东,博士,副研究员,E-mail:luoshudong@caas.cn。
基金资助:
国家蜂产业技术体系（CARS-45）；中国农业科学院科技创新工程（CAAS-ASTIP-2016-IAR）

Survival Risk Analysis Honey bee Workers (Apis mellifera L., Hymenoptera) Exposed to Sub-lethal Concentration of Thiamethoxam

YUE Meng¹, ZONG Fulin^2,3, MA Changsheng¹, WU Jie¹, LUO Shudong¹

1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences/Key Laboratory for Biology of Insect-Pollinator, Ministry of Agriculture, Beijing 100093, China;
2. Institute for the Control of Agrochemicals, Beijing 100026, China;
3. China Agricultural University, Beijing 100083, China

Received:2017-01-25 Online:2017-10-08 Published:2017-10-11

摘要/Abstract

摘要： 噻虫嗪是一类较第一代新烟碱类杀虫剂更广谱、高效、低毒的农药，但它对意大利蜜蜂工蜂的生存风险尚不明确。本研究通过糖水混毒法测定了噻虫嗪对意大利蜜蜂新出房蜂（1 d）、哺育蜂（7 d）和采集蜂（22 d）的48 h急性经口毒性，确定其各自的亚致死浓度LC₅、LC₁₅和LC₂₅，并分别研究了其在3个亚致死浓度下的生存风险。结果表明，新出房蜂、哺育蜂和采集蜂48 h后LC₅₀分别为248.13、64.18和44.20 ng/g。新出房蜂经LC₅和LC₁₅处理后其平均存活寿命与对照均无显著差异，经LC₂₅处理后其平均存活寿命与对照缩短19.0 d。哺育蜂经LC₅处理后其平均存活寿命与对照均无显著差异，经LC₁₅和LC₂₅处理后其平均存活寿命与对照分别缩短9.2和15.0 d。采集蜂经LC₅和LC₁₅处理后其平均存活寿命与对照均无显著差异，经LC₂₅处理后其平均存活寿命与对照缩短10.9 d。上述结果表明噻虫嗪的使用可能会对蜜蜂的生存存在较大风险，因此，建议相关管理部门加强噻虫嗪在中国的田间残留监测，开展半田间和田间条件下噻虫嗪对意大利蜜蜂的风险评价工作。

关键词: 噻虫嗪, 意大利蜜蜂, 工蜂, 存活寿命, 亚致死效应

Abstract: Compared with the first generation neonicotinoids, thiamethoxam is used widely for its higher efficiency, broader spectrum and lower toxicity to mammalian. However, there are no reports on the sublethal effects of thiamethoxam on the honey bees Apis mellifera L., especially on the survival of the workers. Here we assessed the acute oral toxicities of thiamethoxam to the newly emerged bees (1 d), nurses (7 d) and foragers (22 d). Also, the survival risk of exposure to three sub-lethal concentrations (LC₅, LC₁₅ and LC₂₅) of thiamethoxam on different ages of workers was investigated. The results showed that the LC₅₀ for the newly emerged bees, nurses and foragers were 248.13, 64.18 and 44.20 ng/g, respectively. No significant differences in lifespan of the newly emerged bees and the foragers were detected between the control, LC₅ and LC₁₅ treatments, while LC₂₅ treatment reduced lifespan by 19.0 d. For the nurses, the LC₅ treatment didn't shorten bees' lifespan, but the LC₁₅ and LC₂₅ treatments reduced longevity by 9.2 and 15.0 d, respectively. For the foragers, there were no significant differences among the lifespan of the LC₅, LC₁₅ treatments and the control, while the LC₂₅ treatment shortened lifespan by 10.9 d. These results indicate that the extensive application of thiamethoxam has great potential threat on longevity of the honey bees. More attention to monitor the field residual of thiamethoxam and assess the risk of thiamethoxam on honey bees in semi-field and field context is needed.

Key words: thiamethoxam, Apis mellifera, workers, survival analysis, sub-lethal effects

中图分类号:

S476

岳孟, 宗伏霖, 马昌盛, 吴杰, 罗术东. 亚致死浓度噻虫嗪对意大利蜜蜂工蜂的生存风险分析[J]. 中国生物防治学报, 2017, 33(5): 638-643.

YUE Meng, ZONG Fulin, MA Changsheng, WU Jie, LUO Shudong. Survival Risk Analysis Honey bee Workers (Apis mellifera L., Hymenoptera) Exposed to Sub-lethal Concentration of Thiamethoxam[J]. journal1, 2017, 33(5): 638-643.

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亚致死浓度噻虫嗪对意大利蜜蜂工蜂的生存风险分析

Survival Risk Analysis Honey bee Workers (Apis mellifera L., Hymenoptera) Exposed to Sub-lethal Concentration of Thiamethoxam

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