烟管菌M-1对柑橘炭疽病菌的抑制作用及发酵条件优化

doi:10.16409/j.cnki.2095-039x.2018.03.010

摘要/Abstract

摘要： 本文优化了烟管菌Bjerkandera adusta M-1发酵液对柑橘炭疽病Colletotrichum gloeosprioides的抑制发酵条件，为柑橘炭疽病菌生物防治提供研究基础。通过采用平板对峙试验、单因素试验及响应曲面法，研究了烟管菌M-1菌株对柑橘炭疽病菌的拮抗作用。平板对峙试验表明，烟管菌M-1对柑橘炭疽病菌具有明显的抑制作用，抑菌率达65.7%，显著高于化学农药多菌灵和甲基托布津；发酵条件优化后，当C/N 7.5，初始pH 6.5，装瓶量36%，发酵时间22 d，转速180 r/min，温度30℃时，对柑橘炭疽病菌的抑菌率最高，达71.1%。本研究首次发现烟管菌M-1对柑橘炭疽病菌具有很好的拮抗作用，并确定了最优的发酵条件组合，这将为利用烟管菌防治柑橘炭疽病的实际应用奠定理论基础。

关键词: 烟管菌, 响应曲面, 柑橘炭疽病, 发酵液, 拮抗作用

Abstract: In this study, the biocontrol efficiency of Bjerkandera adusta M-1 strain on Colletotrichum gloeosprioides was investigated by the plate confrontation test and fermentation conditions of M-1 were optimized by the single factor test and the response surface methodology. The results showed that M-1 can obviously inhibit the growth of C. gloeosprioides, with the inhibitory rate of 65.7%, significantly higher than Carbendazim and Thiophanate methyl. The optimized fermentation conditions were C/N of 7.5, pH of 6.5, culture volume 36% of bottling capacity, a culture time of 22 d, a rotational speed of 180 r/min, and a culture temperature of 30℃, and the inhibition rate reached 71.1%. In conclusion, it was the first report that B. adusta M-1 strain had a strong antagonistic activity against C. gloeosprioides, and the inhibition rate of fermentation broth was strengthened after the fermentation parameters were optimized. The results laid a theoretical foundation for potential application of M-1 against C. gloeosprioides in practice.

Key words: Bjerkandera adusta, response surface methodology, Colletotrichum gloeosprioides, fermentation broth, antagonism

中图分类号:

S476

汪文强, 李勇, 张旭辉, 魏万玲, 周丰武, 李素平, 李杰. 烟管菌M-1对柑橘炭疽病菌的抑制作用及发酵条件优化[J]. 中国生物防治学报, 2018, 34(3): 423-430.

WANG Wenqiang, LI Yong, ZHANG Xuhui, WEI Wanling, ZHOU Fengwu, LI Suping, LI Jie. Antagonism of Bjerkandera adusta M-1 against Colletotrichum gloeosprioides and Optimization of Fermentation Conditions[J]. journal1, 2018, 34(3): 423-430.

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