棉铃虫CYP9G5基因的克隆、序列分析及溴氰菊酯胁迫表达

doi:10.16409/j.cnki.2095-039x.2018.04.004

摘要/Abstract

摘要： 细胞色素P450是超基因家族，属于生物体内重要三大解毒酶系之一，参与内源物和外源物的合成与氧化代谢。本文通过棉铃虫溴氰菊酯短期胁迫转录组数据分析，克隆获得一条P450基因CYP9G5（GenBank：KM016757.1），编码493个氨基酸，分子量约为57.930 kDa，理论等电点pI 7.16。通过实时荧光定量技术，分析CYP9G5基因在棉铃虫不同组织和溴氰菊酯处理后表达量的变化，发现CYP9G5在头部表达量最高，经过溴氰菊酯处理后CYP9G5表达量也显著升高。推测出CYP9G5极有可能参与棉铃虫对溴氰菊酯的代谢，可能对棉铃虫的耐药性有一定的作用。本研究为进一步探索棉铃虫的抗药性和新农药的开发奠定了基础。

关键词: 细胞色素P450, 棉铃虫, 溴氰菊酯, 实时荧光定量PCR

Abstract: Cytochrome P450 is a supergene family, which belongs to one of the three important detoxification enzymes in vivo and participates in the synthesis and oxidation metabolism of endogenous and exogenous substances. In this paper, a P450 gene CYP9G5 (GenBank:KM016757.1) was cloned through data analysis of the transcription group of H. armigera, encoding 493 amino acids with a molecular weight of about 57.930 kDa, and theoretical isoelectric point pI 7.16. Using the technology of real-time quantitative analysis the CYP9G5 genes in different groups of the Helicoverpa armigera and deltamethrin treatment after expressing quantity change, found CYP9G5 highest expression quantity of the head, after treated with deltamethrin CYP9G5 express volume also increased significantly. It is speculated that CYP9G5 is very likely to be involved in the metabolism of H. armigera to cypermethrin, and may play a role in the resistance of H. armigera. This study lays a foundation for further exploring the resistance of H. armigera and the development of new pesticides.

Key words: Cytochrome P450, Helicoverpa armigera, deltamethrin, Real-time quantitative PCR

中图分类号:

S435.622

张坤, 邓登辉, 郭江龙, 韩卫丽, 吴少英. 棉铃虫CYP9G5基因的克隆、序列分析及溴氰菊酯胁迫表达[J]. 中国生物防治学报, 2018, 34(4): 520-526.

ZHANG Kun, DENG Denghui, GUO Jianglong, HAN Weili, WU Shaoying. Cloning, Sequence Analysis and Expression of Helicoverpa armigera CYP9G5 Gene under Stress of Deltamethrin[J]. journal1, 2018, 34(4): 520-526.

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