肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响

doi:10.16409/j.cnki.2095-039x.2019.01.008

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (1): 24-30.DOI: 10.16409/j.cnki.2095-039x.2019.01.008

肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响

张琪文, 花若晗, 王聪, 林嘉, 宋学森, 张贺贺, 季清娥, 陈湜, 杨建全

福建农林大学益虫研究所/联合国(中国)实蝇防控研究中心/闽台作物有害生物生态防控国家重点实验室/生物农药与化学教育部重点实验室, 福州 350002

收稿日期:2018-08-13 出版日期:2019-02-08 发布日期:2019-01-31
通讯作者: 季清娥,研究员,E-mail:jiqinge@yeah.net。
作者简介:张琪文,硕士研究生,E-mail:344463987@qq.com
基金资助:
国家重点研发计划（2017YFD0202000）

Impacts of Cultivable Gut Microbes on Biological Characteristics of Sterile Males of Bactrocera dorsalis (Hendel)

ZHANG Qiwen, HUA Ruohan, WANG Cong, LIN Jia, SONG Xuesen, ZHANG Hehe, JI Qing'e, CHEN Shi, YANG Jianquan

Institute of Beneficial Insects, College of Plant Protection, Fujian Agriculture and Forestry University/UN(China) Center for Fruit Fly Prevention and Treatment/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 50002, China

Received:2018-08-13 Online:2019-02-08 Published:2019-01-31

摘要/Abstract

摘要： 橘小实蝇是一种对果、蔬产业造成严重经济损失的害虫，应用昆虫不育技术（SIT）防治橘小实蝇是一种切实可行的科学手段，但辐照会使不育雄虫受到一定程度的损伤。本研究通过在饲料中添加抗生素对肠道灭菌处理来探究肠道可培养共生菌对橘小实蝇生物学及形态学参数的影响。结果表明，抗生素处理组在不育雄虫的蛹重、逆境存活率以及竞争交配力等均显著低于对照组。另外，抗生素处理组和对照组不育雄虫的存活率曲线图表明处理组不育雄虫的寿命时长显著低于对照组，利用Weibull分布拟合模型判断得知两组橘小实蝇存活率曲线均符合基本模型中的Ⅰ型。本研究为进一步利用肠道可培养共生菌来改善橘小实蝇不育雄虫的生理健康提供理论基础。

关键词: 橘小实蝇, 抗生素处理, 肠道可培养共生菌, 生物学参数

Abstract: Bactrocera dorsalis (Hendel) is a notorious agricultural pest of fruit and vegetable industry. Sterile insect technique is widely used in control of B. dorsalis, but sterile males may be injured during radiation. In this study, the influence of cultivable gut microbes on biology and morphology of B. dorsalis was investigated through adding two antibiotics to the larval diet. The pupal weight, survival rate and mating competition of the antibiotics- treated sterile males were significantly lower than the control sterile males. Further, although the survival curves of the antibiotics-treated and control sterile males fitted with pattern I of the basic Weibull distribution model, longevity of the antibiotics-treated sterile males was significantly shorter than that of the control. This result suggest that gut microbes can be used to reduce the irradiation-induced damage to Bactrocera dorsalis.

Key words: Bactrocera dorsalis, antibiotic treatment, cultivable gut microbes, biological parameters

中图分类号:

S476

张琪文, 花若晗, 王聪, 林嘉, 宋学森, 张贺贺, 季清娥, 陈湜, 杨建全. 肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响[J]. 中国生物防治学报, 2019, 35(1): 24-30.

ZHANG Qiwen, HUA Ruohan, WANG Cong, LIN Jia, SONG Xuesen, ZHANG Hehe, JI Qing'e, CHEN Shi, YANG Jianquan. Impacts of Cultivable Gut Microbes on Biological Characteristics of Sterile Males of Bactrocera dorsalis (Hendel)[J]. journal1, 2019, 35(1): 24-30.

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肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响

Impacts of Cultivable Gut Microbes on Biological Characteristics of Sterile Males of Bactrocera dorsalis (Hendel)

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