橘小实蝇雌虫对其肠道共生菌发酵液的嗅觉行为反应

doi:10.16409/j.cnki.2095-039x.2019.04.007

中国生物防治学报 ›› 2019, Vol. 35 ›› Issue (6): 891-899.DOI: 10.16409/j.cnki.2095-039x.2019.04.007

橘小实蝇雌虫对其肠道共生菌发酵液的嗅觉行为反应

林嘉¹, 蔡普默^1,3, 张贺贺¹, 宋学森¹, 张琪文¹, 仪传冬¹, 敖国富^1,4, 杨建全¹, 季清娥^1,2

1. 福建农林大学益虫研究所/联合国(中国)实蝇防控研究中心/闽台作物有害生物生态防控国家重点实验室/生物农药与化学生物学教育部重点实验室, 福州 350002;
2. 福建农林大学作物生物技术福建省高校重点实验室, 福州 350002;
3. 武夷学院茶与食品学院, 武夷山 354300;
4. 安顺学院农学院, 安顺 561000

收稿日期:2019-03-13 出版日期:2019-12-08 发布日期:2019-12-18
通讯作者: 杨建全,博士,研究员,E-mail:jqyang2003@163.com;季清娥,博士,研究员,E-mail:Jiqinge@yeah.net
作者简介:林嘉,硕士研究生,E-mail:Linjia25@foxmail.com。
基金资助:
国家重点研发计划（2017YFD0202000）

The Olfactory Behavioral Response of Bactrocera dorsalis Females to Fermented Solution of Their Gut Endosymbionts

LIN Jia¹, CAI Pumo^1,3, ZHANG Hehe¹, SONG Xuesen¹, ZHANG Qiwen¹, YI Chuandong¹, AO Guofu^1,4, YANG Jianquan¹, JI Qing'e^1,2

1. Institute of Beneficial Insects, College of Plant Protection, Fujian Agriculture and Forestry University/UN(China) Center for Fruit Fly Prevention and Treatment/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China;
2. Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. Department of Horticulture, College of Tea and Food Science, Wuyi University, Wuyishan 354300, China;
4. College of Agriculture, Anshun University, Anshun 561000, China

Received:2019-03-13 Online:2019-12-08 Published:2019-12-18

摘要/Abstract

摘要： 肠道微生物潜在的生防价值为研究橘小实蝇治理提供了一个新的思路。本研究利用Y型嗅觉仪测试了橘小实蝇肠道菌发酵原液及其高压灭活上清液对寄主雌虫在10、20以及30 min嗅觉行为的影响，结果表明阴沟肠杆菌（F8）、肠杆菌属（F13）、产气克雷伯氏菌（F26）、霍氏肠杆菌（F67）发酵原液以及阴沟肠杆菌（F8）、肺炎克雷伯氏菌（F10）、Pseudocitrobacter anthropi（F16）、粪肠球菌（F18）、赫尔曼亚特兰大杆菌（F31）、蜡样芽胞杆菌（F45）、铜绿假单胞菌（F50）、阴沟肠杆菌（F59）高压灭活上清液对橘小实蝇雌虫具有显著的引诱效果，P. anthropi（F16）发酵原液对雌虫具有显著的忌避作用；此外，橘小实蝇对不同的肠道菌的反应时间不同。本研究明确了橘小实蝇雌虫对其肠道菌发酵原液、高压灭活上清液的嗅觉反应，为开发雌虫引诱或忌避剂提供了依据。

关键词: 橘小实蝇, 雌虫, 肠道菌, 嗅觉行为

Abstract: The biocontrol potential value of gut bacteria provides a new insight for the control of Bactrocera dorsalis (Hendel). In this study, Y-tube olfactometer was used to investigate the effects of the fermented solution and autoclaved supernatant of gut endosymbionts on the olfactory responses of their host, female B. dorsalis, within 10, 20 and 30 mins. The results showed that the fermented solution of Enterobacter cloacae (F8), Enterobacter sp. (F13), Klebsiella aerogenes (F26), and E. hormaechei (F67), and the autoclaved supernatant of E. cloacae (F8), K. pneumonia (F10), Pseudocitrobacter anthropi (F16), Enterococcus faecalis (F18), Atlantibacter hermannii (F31), Bacillus cereus (F45), Pseudomonas aeruginosa (F50), and E. cloacae (F59) exhibited significant attraction to B. dorsalis females. Greater repellent effect was observed in the fermented solution of P. anthropi (F16). Furthermore, B. dorsalis showed varied responding time to different bacteria species. The olfactory responses of female B. dorsalis to their intestinal tract bacteria identified in the paper will provide the theoretical basis for exploiting new attractive lure or repellent against female B. dorsalis.

Key words: Bactrocera dorsalis, female, gut bacteria, olfactory behavioral response

中图分类号:

S436.6

林嘉, 蔡普默, 张贺贺, 宋学森, 张琪文, 仪传冬, 敖国富, 杨建全, 季清娥. 橘小实蝇雌虫对其肠道共生菌发酵液的嗅觉行为反应[J]. 中国生物防治学报, 2019, 35(6): 891-899.

LIN Jia, CAI Pumo, ZHANG Hehe, SONG Xuesen, ZHANG Qiwen, YI Chuandong, AO Guofu, YANG Jianquan, JI Qing'e. The Olfactory Behavioral Response of Bactrocera dorsalis Females to Fermented Solution of Their Gut Endosymbionts[J]. Chinese Journal Of Biological Control, 2019, 35(6): 891-899.

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橘小实蝇雌虫对其肠道共生菌发酵液的嗅觉行为反应

The Olfactory Behavioral Response of Bactrocera dorsalis Females to Fermented Solution of Their Gut Endosymbionts

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