大豆蚜Aghsp75基因对吡虫啉及温度胁迫的应激表达

doi:10.16409/j.cnki.2095-039x.2020.03.004

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (6): 913-919.DOI: 10.16409/j.cnki.2095-039x.2020.03.004

• 研究论文 • 上一篇

大豆蚜Aghsp75基因对吡虫啉及温度胁迫的应激表达

王晓云¹, 李双宇¹, 姚磊², 刘艳¹, 陈雅茹¹, 杨洪佳¹, 樊东¹

1. 东北农业大学农学院, 哈尔滨 150030;
2. 绥芬河海关, 绥芬河 157399

收稿日期:2020-01-27 发布日期:2021-01-09
通讯作者: 樊东,博士,教授,E-mail:dnfd@163.com。
作者简介:王晓云,硕士,高级实验师,E-mail:67722142@qq.com
基金资助:
国家重点研发计划（2018YFD0201000）；现代农业产业技术体系建设专项资金（CARS-04）

Differential Expression of Aphis glycines Gene Aghsp75 in Response to Imidacloprid and Temperature Stress

WANG Xiaoyun¹, LI Shuangyu¹, YAO Lei², LIU Yan¹, CHEN Yaru¹, YANG Hongjia¹, FAN Dong¹

1. College of Agronomy, Northeast Agricultural University, Harbin 150030, China;
2. Suifenhe Customs, Suifenhe 157399, China

Received:2020-01-27 Published:2021-01-09

摘要/Abstract

摘要： 热激蛋白在昆虫抵御温度胁迫和药剂胁迫反应中具有重要作用。本试验通过高通量测序获得一条大豆蚜热激蛋白基因的cDNA序列，该序列全长2982 bp，含1个长度为2052 bp的开放阅读框，编码683个氨基酸组成的多肽，多肽等电点约为6.30，分子质量约为77.8 kDa；推导的氨基酸序列与棉蚜Aphis gossypii HSP75同源性高达99.12%，属于hsp 75家族。我们把该基因定名为Aghsp75，已提交至GenBank（登录号为MN068810）。Aghsp75通过不同浓度吡虫啉药剂和不同温度胁迫成蚜后发现，经LC₅₀吡虫啉浓度胁迫3、6和24 h时以及在LC₃₀浓度吡虫啉胁迫12 h时该基因表达量显著升高；经6℃处理6 h时以及36℃处理3 h和6 h时该基因表达量显著升高。本研究表明该基因可能参与大豆蚜的抗逆过程，为利用分子生物技术手段防治大豆蚜提供理论保障。

关键词: 大豆蚜, 热激蛋白hsp75, 药剂胁迫, 温度胁迫

Abstract: Heat shock proteins (HSP) play important roles in insect resistance to temperature and insecticide stress. A heat shock protein cDNA sequence of Aphis glycines was obtained by high throughput sequencing, which was a 2982 bp full-length sequence and contained a 2052 bp open reading frame. It encoded a polypeptide consisting of 683 amino acids. The isoelectric point of the polypeptide was about 6.30 and the molecular weight was about 77.8 kDa. The deduced amino acid sequence shared 99.12% identity with Aphis gossypii HSP75. The cDNA sequence was named Aghsp75. The GenBank accession number was MN068810. The relative expression of Aghsp75 was significantly increased after 3, 6 and 24 h induction by imidacloprid LC₅₀ and 12 h induction by imidacloprid LC₃₀, or after 6 h treatment at 6 ℃ and 3 h and 6 h treatment at 36 ℃. This study suggests that Aghsp75 may be involved in the stress resistance of A. glycines and provides theoretical support for the control of A. glycines by molecular biotechnology.

Key words: Aphis glycines, heat shock protein hsp75, insecticide stress, temperature stress

中图分类号:

S435.65

王晓云, 李双宇, 姚磊, 刘艳, 陈雅茹, 杨洪佳, 樊东. 大豆蚜Aghsp75基因对吡虫啉及温度胁迫的应激表达[J]. 中国生物防治学报, 2020, 36(6): 913-919.

WANG Xiaoyun, LI Shuangyu, YAO Lei, LIU Yan, CHEN Yaru, YANG Hongjia, FAN Dong. Differential Expression of Aphis glycines Gene Aghsp75 in Response to Imidacloprid and Temperature Stress[J]. Chinese Journal of Biological Control, 2020, 36(6): 913-919.

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大豆蚜Aghsp75基因对吡虫啉及温度胁迫的应激表达

Differential Expression of Aphis glycines Gene Aghsp75 in Response to Imidacloprid and Temperature Stress

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