盾壳霉对油菜长效专用配方肥料的敏感性评价

doi:10.16409/j.cnki.2095-039x.2020.03.025

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (3): 465-471.DOI: 10.16409/j.cnki.2095-039x.2020.03.025

盾壳霉对油菜长效专用配方肥料的敏感性评价

刘小凡, 谢甲涛, 付艳苹, 姜道宏, 程家森

华中农业大学植物科学与技术学院/微生物学国家重点实验室/湖北省作物病害监测和安全控制重点实验室, 武汉 430070

收稿日期:2019-05-23 出版日期:2020-06-08 发布日期:2020-06-12
通讯作者: 程家森,教授,E-mail:jiasencheng@mail.hzau.edu.cn。
作者简介:刘小凡,硕士研究生,E-mail:1192803396@qq.com
基金资助:
国家重点研发计划（2018YFD0200900）；现代产业技术体系岗位科学家科研专项（CARS-13）

Sensitivity Evaluation of Coniothyrium minitans to Rapeseed Slow-released Special Formula Fertilizer

LIU Xiaofan, XIE Jiatao, FU Yanping, JIANG Daohong, CHENG Jiasen

State Key Laboratory of Agricultural Microbiology/Key Laboratory of Plant Pathology of Hubei Province/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2019-05-23 Online:2020-06-08 Published:2020-06-12

摘要/Abstract

摘要： 油菜菌核病是油菜生产中的重要病害，盾壳霉是核盘菌的重寄生真菌，在菌核病防治方面具有重要的生防潜力。为了明确盾壳霉与油菜长效专用配方肥料混合施用的可行性，本文研究了盾壳霉对油菜长效专用配方肥的敏感性。结果发现，低浓度的油菜长效专用配方肥（1.5和7.5 mg/mL）对盾壳霉菌丝生长、菌落及菌丝尖端形态和分生孢子萌发等无明显影响，高浓度的油菜长效专用配方肥对盾壳霉生长和孢子萌发有一定影响，在饱和浓度（560 mg/mL）条件下的油菜长效专用配方肥，24 h盾壳霉孢子的萌发率为0.83%，然而在96 h时萌发率可达到95%；油菜长效专用配方肥对盾壳霉产孢和寄生致腐菌核的能力无明显影响，寄生菌核30 d后，致腐指数均在50以上。研究结果表明，在田间生产中，盾壳霉可以与油菜长效专用配方肥料混合施用，达到轻简化栽培的目的。

关键词: 盾壳霉, 核盘菌, 油菜菌核病, 油菜长效专用配方肥料, 敏感性

Abstract: Sclerotinia is an important disease in rapeseed (Brassica napus) production. Coniothyrium minitans is a mycoparasite of the plant-pathogenic fungus Sclerotinia sclerotiorum, which has important biocontrol potential in the prevention and treatment of sclerotinia stem rot. In order to clarify the feasibility of the mixture of C. minitans and rapeseed slow-released special formula fertilizer (RSF), the sensitivity of C. minitans to RSF was evaluated in this paper. The results showed that the low concentration of RSF (1.5 mg/mL and 7.5 mg/mL) had no significant effect on the growth, colony morphology, branch pattern and conidia germination of C. minitans, while high concentration of RSF had some effects on the growth and conidia germination of C. minitans. Under the condition of saturated concentration of RSF (560 mg/mL), the germination rate of C. minitans conidia was only 0.83% at 24 hours post inoculation (hpi) on culture medium, however, the germination rate reached to 95% at 96 hpi. Furthermore, the fertilizer had no obvious effect on conidiation and parasitic activity to sclerotia of S. sclerotiorum. In the presence of RSF, ZS-1 was still capable of colonizing sclerotia and the sclerotia rot index exceeded 50 after 30 days. Taken together, our results showed that C. minitans can be mixed with rapeseed slow-released special formula fertilizer in field production.

Key words: Coniothyrium minitans, Sclerotinia sclerotiorum, rapeseed sclerotinia rot, rapeseed slow-released special formula fertilizer, sensitivity

中图分类号:

S476

刘小凡, 谢甲涛, 付艳苹, 姜道宏, 程家森. 盾壳霉对油菜长效专用配方肥料的敏感性评价[J]. 中国生物防治学报, 2020, 36(3): 465-471.

LIU Xiaofan, XIE Jiatao, FU Yanping, JIANG Daohong, CHENG Jiasen. Sensitivity Evaluation of Coniothyrium minitans to Rapeseed Slow-released Special Formula Fertilizer[J]. Chinese Journal Of Biological Control, 2020, 36(3): 465-471.

参考文献

[1] Bolton M D, Thomma B P, Nelson B D. Sclerotinia sclerotiorum, (Lib) de Bary:biology and molecular traits of a cosmopolitan pathogen[J]. Molecular Plant Pathology, 2006, 7(1):1-16.
[2] Boland G J, Hall R. Index of plant hosts of Sclerotinia sclerotiorum[J]. Canadian Journal Plant Pathology, 1994, 16:93-108.
[3] Wang Y, Sun Y, Zhang Y, et al. Sensitivity and biochemical characteristics of Sclerotinia sclerotiorum to propamidine[J]. Pesticide Biochemistry and Physiology, 2016, 135:82.
[4] Abawi G S. Epidemiology of diseases caused by Sclerotinia species[J]. Phytopathology, 1979, 69(8):899.
[5] Li G Q, Huang H J, Miao H J, et al. Biological control of Sclerotinia diseases of rapeseed by aerial applications of the mycoparasite Coniothyrium minitans[J]. European Journal of Plant Pathology, 2006, 114(4):345-355.
[6] 羊国根, 程家森. 核盘菌致病机理研究进展[J]. 生物技术通报, 2018, 309(4):15-21.
[7] Xu D, Pan Y, Zhang H, et al. Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China[J]. Genetics and Molecular Research, 2015, 14(4):16627.
[8] Adams P B, Ayers W A. Ecology of Sclerotinia species[J]. Phytopathology, 1979, 69(8):896.
[9] Clarkson J P, Phelps K, Whipps J M, et al. Forecasting Sclerotinia disease on lettuce:A predictive model for carpogenic germination of Sclerotinia sclerotiorum sclerotia[J]. Phytopathology, 2007, 97(5):621-631.
[10] 游景茂, 谢甲涛, 付艳苹, 等. 除草剂精禾草克和乙草胺对油菜菌核病生防菌盾壳霉生长和寄生的影响[J]. 中国生物防治, 2010, 26(3):340-346.
[11] Campbell W A. A new species of Coniothyrium parasitic on sclerotia[J]. Mycologia, 1947, 39(2):190-195.
[12] Budge S P, Whipps J M. Glasshouse trials of Coniothyrium minitans and Trichoderma species for the biological control of Sclerotinia sclerotiorum in celery and lettuce[J]. Plant Pathology, 1991, 40:59-66.
[13] Jones E E, Rabeendran N, Stewart A. Biocontrol of Sclerotinia sclerotiorum infection of cabbage by Coniothyrium minitans and Trichoderma spp.[J]. Biocontrol Science and Technology, 2014, 24(12):20.
[14] 强翠翠, 程家森, 谢甲涛, 等. 盾壳霉对2种除草剂的敏感性评价[J]. 华中农业大学学报(自然科学版), 2018, 37(5):25-31.
[15] Budge S P, McQuilken M P, Fenlon J S, et al. Use of Coniothyrium minitans and Gliocladium virens for biological control of Sclerotinia sclerotiorum in glasshouse lettuce[J]. Biological Control, 1995, 5(4):513-522.
[16] Elsheshtawi M, Elkhaky M T, Sayed S R, et al. Integrated control of white rot disease on beans caused by Sclerotinia sclerotiorum using contans and reduced fungicides application[J]. Saudi Journal of Biological Sciences, 2016, 24(2):405-409.
[17] 周鹂, 鲁剑巍, 刘涛, 等. 油菜长效专用配方肥施用效果研究[J]. 中国土壤与肥料, 2015(4):71-75.
[18] Jiang D H, Li G Q, Yi X H, et al. Characterization of the sclerotial parasite Coniothyrium minitansⅡ.comparison of the cultural characteristics and the abilities to parasitize the sclerotia of Sclerotinia sclerotiorum[J]. Journal of Huazhong Agricultural University, 1996, 15(3):229-232.
[19] 杨龙, 罗倩, 李国庆, 等. 盾壳霉与硼肥的相容性及其混用对核盘菌菌核萌发的控制[C]//中国植物病理学会2011年学术年会论文集, 2011.
[20] Yang L, Li G, Zhang J, et al. Compatibility of Coniothyrium minitans with compound fertilizer in suppression of Sclerotinia sclerotiorum[J]. Biological Control, 2011, 59(2):221-227.

盾壳霉对油菜长效专用配方肥料的敏感性评价

Sensitivity Evaluation of Coniothyrium minitans to Rapeseed Slow-released Special Formula Fertilizer

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