苏云金杆菌Bt-59菌株Cry/Cyt毒素蛋白鉴定及杀虫活性

doi:10.16409/j.cnki.2095-039x.2020.03.024

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (3): 458-464.DOI: 10.16409/j.cnki.2095-039x.2020.03.024

苏云金杆菌Bt-59菌株Cry/Cyt毒素蛋白鉴定及杀虫活性

韩光杰^1,2, 徐灵环^1,2, 李传明¹, 刘琴¹, 彭琦², 徐彬¹, 宋福平², 徐健¹

1. 江苏里下河地区农业科学研究所/国家农业微生物扬州观测实验站, 扬州 225007;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193

收稿日期:2019-10-28 出版日期:2020-06-08 发布日期:2020-06-12
通讯作者: 徐健,研究员,E-mail:bio-xj@163.com;宋福平,研究员,E-mail:fpsong@ippcaas.cn。
作者简介:韩光杰,副研究员,E-mail:hanguangjie177@163.com;徐灵环,硕士研究生,E-mail:1663443125@qq.com。
基金资助:
国家自然科学基金（31071740）；植物病虫害生物学国家重点实验室开放课题（SKLOF201807）；省部共建国家重点实验室培育基地江苏省食品质量安全重点实验室开放课题（028074911709）

Characteristics of Cry and Cyt Proteins and Insecticidal Activity in Bacillus thuringiensis Bt-59

HAN Guangjie^1,2, XU Linghuan^1,2, LI Chuanming¹, LIU Qin¹, PENG Qi², XU Bin¹, SONG Fuping², XU Jian¹

1. Jiangsu Lixiahe District Institute of Agriculture Sciences/National Agricultural Experimental Station for Agricultural Microbiology, Yangzhou 225007, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2019-10-28 Online:2020-06-08 Published:2020-06-12

摘要/Abstract

摘要： 苏云金杆菌以色列亚种（Bacillus thuringiensis subsp.israelensis，Bti）是防治蚊幼虫的重要病原细菌，不同菌株间毒素蛋白种类和分布不尽相同。本研究利用苏云金杆菌以色列亚种Bt-59菌株的全基因组数据，对潜在的毒素基因种类、分布及菌株杀虫活性进行了分析。结果表明，Bt-59菌株除了含有Bti标准菌株主要毒素基因外，还含有Cry4-like基因，且Cry10Aa和Cyt1Ca基因在质粒4上发生了易位。质粒5上的Cry60Aa和Cry60Ba基因编码的毒素蛋白含有ETX_MTX2结构域，并与苏云金杆菌jegathesan亚种的同源蛋白一致性为100%。Cyt1Ca蛋白除了含有Bac_thur_toxin结构域外，还含有2个串联的RicinB_lectin_2结构域。菌株Bt-59对蚊幼虫具有较高的杀虫活性，对白纹伊蚊Aedes albopictus和淡色库蚊Culex pipiens pallens的LC₅₀分别为1.239 μL/L和0.341 μL/L。研究结果明确了菌株Bt-59毒素蛋白的种类和特征，为菌株遗传改良提供理论基础。

关键词: 苏云金杆菌, Cry蛋白, Cyt蛋白, 蚊子, 生物防治

Abstract: Bacillus thuringiensis subsp. israelensis (Bti) is an important pathogen for control of mosquito larvae. The types and distribution of toxin proteins are diverse among different strains. In this study, the types and characteristics of toxin genes and insecticidal activity of Bt-59 strain were determined. Bt-59 is different from Bti standard strains in that it harbors a Cry4-like gene and the positions of Cry10Aa and Cyt1Ca are translocated on plasmid 4. The toxin proteins encoded by Cry60Aa and Cry60Ba genes on plasmid 5 contain the ETX_MTX2 domain, showing identity of 100% to homologous proteins of B. thuringiensis subsp. jegathesan. Besides the Bac_thur_toxin domain, Cyt1Ca protein also contains two RicinB_lectin_2 domains in tandem. Bt-59 has high insecticidal activity against mosquito larvae, with LC₅₀ for Aedes albopictus and Culex pipiens pallens of 1.239 μL/L and 0.341 μL/L, respectively. Our research provides a theoretical basis for the genetic improvement of the Bt-59 strain.

Key words: Bacillus thuringiensis, Cry, Cyt, mosquito, biocontrol

中图分类号:

Q933

韩光杰, 徐灵环, 李传明, 刘琴, 彭琦, 徐彬, 宋福平, 徐健. 苏云金杆菌Bt-59菌株Cry/Cyt毒素蛋白鉴定及杀虫活性[J]. 中国生物防治学报, 2020, 36(3): 458-464.

HAN Guangjie, XU Linghuan, LI Chuanming, LIU Qin, PENG Qi, XU Bin, SONG Fuping, XU Jian. Characteristics of Cry and Cyt Proteins and Insecticidal Activity in Bacillus thuringiensis Bt-59[J]. Chinese Journal Of Biological Control, 2020, 36(3): 458-464.

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苏云金杆菌Bt-59菌株Cry/Cyt毒素蛋白鉴定及杀虫活性

Characteristics of Cry and Cyt Proteins and Insecticidal Activity in Bacillus thuringiensis Bt-59

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