靶标寄主害虫提取物驯化对松毛虫赤眼蜂寄主选择偏好的影响

doi:10.16409/j.cnki.2095-039x.2021.01.002

摘要/Abstract

摘要： 柞蚕卵繁育的松毛虫赤眼蜂已被用于防治多种鳞翅目害虫。为改善雌蜂对害虫的嗜好性，本研究以亚洲玉米螟卵、幼虫和雌蛾腹部提取液及正己烷对照溶液，在孤雌产雌品系和两性品系子代蜂幼期及羽化前期施加气味刺激，调查子代雌蜂对玉米螟卵的选择偏好。结果发现，对照组孤雌产雌品系雌蜂对玉米螟卵选择率显著高于两性品系；经提取液驯化的两性品系雌蜂对玉米螟卵选择率显著高于对照；雌蛾腹部提取液处理的两性品系雌蜂对玉米螟卵的选择率和瞬时选择倾向最高，但不同提取液对孤雌产雌品系雌蜂的选择率和瞬时选择倾向无显著影响；提取液和蜂品系对雌蜂卵块驻留时间无显著影响。本研究将为改善寄生蜂对寄主害虫的靶向性提供参考。

关键词: 松毛虫赤眼蜂, 寄主偏好, 孤雌生殖, 化学遗赠假说, 规模化繁育

Abstract: The egg parasitoid, Trichogramma dendrolimi, successfully mass-reared on the factitious eggs of Chinese oak silk moth, Antheraea pernyi, has been widely used as an effective biological control agent against multiple lepidopteran pests. However, the preference of T. dendrolimi for the target host pest will decline as the wasps are reared on A. pernyi eggs for a long time. This study aimed to examine the preference of both bisexual Wolbachia-uninfected and thelytokous Wolbachia-infected lines of T. dendrolimi for the target pest eggs. The T. dendrolimi offspring was treated by the odour of the extracts of Ostrinia furnacalis eggs, larvae, and abdomen scales. The host selection of treated offspring wasps was investigated between A. pernyi eggs and O. furnacalis eggs. The results showed that, in the control group, thelytokous females showed a higher preference for O. furnacalis eggs than bisexual females. Regardless of different extract, the bisexual females treated by the extracts exhibited higher preference for O. furnacalis eggs than the control females. The bisexual females treated by the extract of the abdominal scales of O. furnacalis female moths showed the highest selection and instantaneous preference for O. furnacalis eggs. However, the selection rate and instantaneous preference of thelytokous females were not influenced by the extracts. The residence duration of females was not affected by Trichogramma line and the extracts. The results provide a new insight in the improvement of the control efficacy of mass-reared T. dendrolimi wasps on the target pest.

Key words: Trichogramma dendrolimi, host preference, parthenogenesis, chemical legacy hypothesis, mass- rearing

中图分类号:

S476.3

张柱亭, 英美, 周金成, 董辉. 靶标寄主害虫提取物驯化对松毛虫赤眼蜂寄主选择偏好的影响[J]. 中国生物防治学报, 2021, 37(1): 70-76.

ZHANG Zhuting, YING Mei, ZHOU Jincheng, DONG Hui. Effects of Acclimatization to the Extracts from Target Pest on Host Preference of Trichogramma dendrolimi[J]. Chinese Journal of Biological Control, 2021, 37(1): 70-76.

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