产酶溶杆菌OH11中LysR和GntR家族基因的功能分析

doi:10.16409/j.cnki.2095-039x.2021.01.003

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (1): 130-138.DOI: 10.16409/j.cnki.2095-039x.2021.01.003

• 研究论文 • 上一篇

产酶溶杆菌OH11中LysR和GntR家族基因的功能分析

林龙¹, 葛程程¹, 蒋建东², 钱国良¹

1. 南京农业大学植物保护学院, 南京 210095;
2. 南京农业大学生命科学学院, 南京 210095

收稿日期:2020-05-30 发布日期:2021-02-23
通讯作者: 钱国良,博士,教授,E-mail:glqian@njau.edu.cn。
作者简介:林龙,博士,E-mail:linlong@njau.edu.cn
基金资助:
江苏省自然科学基金（BK20190026，BK20181325）；国家自然科学基金（31872016，32001955）；中央高校基本科研业务费专项资金（KJJQ202001，KYT201805，KYTZ201403，KYT202001，KYXK202012）；江苏省农业科技自主创新资金（CX（18）1003）

Function Analysis of LysR and GntR Family Genes in Lysobacter enzymogenes OH11

LIN Long¹, GE Chengcheng¹, JIANG Jiandong², QIAN Guoliang¹

1. College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
2. College of Life Science, Nanjing Agricultural University, Nanjing 210095, China

Received:2020-05-30 Published:2021-02-23

摘要/Abstract

摘要： 产酶溶杆菌Lysobacter enzymogenes OH11可以通过蹭行运动（Twitching motility）接近并附着在病原真菌和卵菌上，进而分泌各种胞外酶和次级代谢产物抑制病原菌的生长，其中热稳定抗真菌因子（heat-stable antifungal factor，HSAF）是一种新型的抗真菌代谢物，具有研发为新型生防杀真菌剂的潜力。转录因子在基因的转录调控中起到关键的作用，鉴于LysR和GntR家族的转录因子在病原细菌中对运动性和致病性具有重要调控作用，本研究在OH11基因组中选取了3个LysR家族蛋白（Le1404、Le2114和Le3293）以及2个GntR蛋白（Le1155和Le1310）的编码基因进行敲除，构建相应的突变体，以明确它们是否可以调控OH11的蹭行运动和HSAF的合成。结果表明，在OH11中分别敲除Le2114和Le1155均导致该菌株丧失蹭行运动；而敲除Le1155能显著降低HSAF的产量，说明Le2114可以特异地调控蹭行运动，而Le1155可以调控蹭行运动和HSAF合成两个生理过程。

关键词: 产酶溶杆菌, 蹭行运动, HSAF, LysR, GntR

Abstract: Lysobacter enzymogenes OH11 can approach and adhere to the hypha of pathogenic fungi and oomycetes through twitching motility. Then OH11 secretes enzymes and secondary metabolites including HSAF (heat-stable antifungal factor), which is a new type of anti-fungal secondary metabolite and a potential resource for new bio-control fungicide, to inhibit pathogen growth. Transcription factor (TF) plays a key role in regulation of gene expression. LysR and GntR TF family regulate motility and pathogenicity in pathogenic bacteria. In this paper, we knocked out encoding genes of 3 LysR TFs (Le1404, Le2114 and Le3293) and 2 GntR TFs (Le1155 and Le1310) to investigate whether they regulate twitching motility and HSAF biosynthesis. ΔLe2114 and ΔLe1155 showed defect in twitching motility; ΔLe1155 produced lower amount of HSAF compared with wild-type. These results indicated Le2114 specifically regulated twitching motility and Le1155 regulated both twitching motility and HSAF biosynthesis.

Key words: Lysobacter enzymogenes, twitching motility, HSAF, LysR, GntR

中图分类号:

S476

林龙, 葛程程, 蒋建东, 钱国良. 产酶溶杆菌OH11中LysR和GntR家族基因的功能分析[J]. 中国生物防治学报, 2021, 37(1): 130-138.

LIN Long, GE Chengcheng, JIANG Jiandong, QIAN Guoliang. Function Analysis of LysR and GntR Family Genes in Lysobacter enzymogenes OH11[J]. Chinese Journal of Biological Control, 2021, 37(1): 130-138.

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产酶溶杆菌OH11中LysR和GntR家族基因的功能分析

Function Analysis of LysR and GntR Family Genes in Lysobacter enzymogenes OH11

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