产酶溶杆菌OH11代谢产物HSAF对梨树腐烂病的防治效果

doi:10.16409/j.cnki.2095-039x.2017.01.016

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (1): 114-120.DOI: 10.16409/j.cnki.2095-039x.2017.01.016

产酶溶杆菌OH11代谢产物HSAF对梨树腐烂病的防治效果

程超¹, 赵延存², 李红旭³, 何锋², 曹素芳³, 杨晓蕾¹, 钱国良¹, 刘凤权^1,2

1. 南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 南京 210095;
2. 江苏省农业科学院植物保护研究所, 南京 210014;
3. 甘肃省农业科学院林果花卉研究所, 兰州 730070

收稿日期:2016-05-06 出版日期:2017-02-08 发布日期:2017-01-24
通讯作者: 刘凤权,研究员,E-mail:fqliu20011@sina.com
作者简介:程超,硕士研究生,E-mail:chenchao950@sina.com
基金资助:
江苏省农业科技自主创新资金项目（CX-16-1049）；江苏省农业科学院基本科研业务专项（ZX-15-1006）；国家公益性行业（农业）科研专项（201203034）；国家现代农业产业技术体系（CARS-29）；国家“948”项目（2014-Z24）；江苏省科技支撑计划（BE2015354、BE2014386）

Control Effect of HSAF from Lysobacter enzymogenes OH11 on Pear Valsa Canker

CHENG Chao¹, ZHAO Yancun², LI Hongxu³, HE Feng², CAO Sufang³, YANG Xiaolei¹, QIAN Guoliang¹, LIU Fengquan^1,2

1. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agriculture University, Nanjing 210095, China;
2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
3. Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China

Received:2016-05-06 Online:2017-02-08 Published:2017-01-24

摘要/Abstract

摘要： HSAF（heat stable antifungal factor）是产酶溶杆菌Lysobacter enzymogenes OH11中分离鉴定的一种小分子广谱抗真菌和卵菌物质，为进一步明确HSAF防治梨树腐烂病的潜力，分别采用生长速率测定法、显微镜观察法和刮治涂抹法测定了HSAF对梨树腐烂病菌的室内毒力以及对梨树腐烂病的田间防治效果。结果表明，HSAF对梨树腐烂病菌有较强的生长抑制作用，EC₅₀值为0.5 μg/mL；HSAF可导致梨树腐烂病菌菌丝体出现扭曲、近顶端处分枝和顶端肿胀等畸形现象；40 μg/mL HSAF凝胶剂对梨树腐烂病的涂治愈合率可达81.3%，与2.12%腐植酸铜水剂的涂治愈合率（85.3%）无显著差异；对于分枝裂口或冻伤处、向阴、主杆或中心枝上的病疤，经40 μg/mL HSAF凝胶剂涂抹后的愈合率高于2.12%腐植酸铜水剂。对于剪锯口处和向阳或分枝上的梨树腐烂病病疤，经2.12%腐植酸铜水剂涂抹后的愈合率高于40 μg/mL HSAF凝胶剂；两种药剂对不同位置病疤的涂治效果不同，可作互补使用。本研究结果为梨树腐烂病的田间防治提供了一种新的生防制剂，同时也可望为其他果树腐烂病害的防治提供生防药剂资源。

关键词: HSAF, 梨树腐烂病, 防治效果

Abstract: Control effects of HSAF (heat stable antifungal factor) from Lysobacter enzymogenes OH11 on pear valsa canker (Valsa pyri) were evaluated by mycelial morphology observation and HSAF gel smeared on canker scars under indoor and field conditions. Results showed that HSAF had strong inhibition on mycelial growth of V. pyri with the EC₅₀ value of 0.5 μg/mL. HSAF also resulted in abnormal mycelial morphology, such as curling, branching spots near the tip of mycelia and tip swollen. There were no significant difference between 40 μg/mL HSAF gel and 2.12% copper humic acid water agent treatments, with healing rate of canker scars of 81.3% and 85.3%, respectively. Moreover, 40 μg/mL HSAF gel showed better control effect than that of 2.12% copper humic acid water agent when smeared on canker scars that caused by cold injury or cleft, or canker scars lied in shady side, trunk or center main trunk. However, the control effect of 2.12% copper humic acid water agent was better than that of 40 μg/mL HSAF gel when smeared on the other kinds of canker scars. This study provides a novel biological agent for the control of pear valsa canker.

Key words: HSAF, pear valsa canker, control effect

中图分类号:

S476

程超, 赵延存, 李红旭, 何锋, 曹素芳, 杨晓蕾, 钱国良, 刘凤权. 产酶溶杆菌OH11代谢产物HSAF对梨树腐烂病的防治效果[J]. 中国生物防治学报, 2017, 33(1): 114-120.

CHENG Chao, ZHAO Yancun, LI Hongxu, HE Feng, CAO Sufang, YANG Xiaolei, QIAN Guoliang, LIU Fengquan. Control Effect of HSAF from Lysobacter enzymogenes OH11 on Pear Valsa Canker[J]. journal1, 2017, 33(1): 114-120.

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产酶溶杆菌OH11代谢产物HSAF对梨树腐烂病的防治效果

Control Effect of HSAF from Lysobacter enzymogenes OH11 on Pear Valsa Canker

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