海南槟榔园昆虫病原线虫对红脉穗螟的致病力

doi:10.16409/j.cnki.2095-039x.2021.03.037

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (3): 431-435.DOI: 10.16409/j.cnki.2095-039x.2021.03.037

• 海南省热带作物主要虫害绿色防控专栏 • 上一篇

海南槟榔园昆虫病原线虫对红脉穗螟的致病力

钟宝珠, 吕朝军, 李朝绪, 覃伟权

中国热带农业科学院椰子研究所/海南省槟榔产业工程研究中心, 文昌 571339

收稿日期:2020-09-20 发布日期:2021-06-16
通讯作者: 覃伟权,E-mail:qwq268@163.com;吕朝军,E-mail:lcj5783@126.com。
作者简介:钟宝珠,硕士,副研究员,E-mail:baozhuz@163.com。
基金资助:
海南省重大科技计划（zdkj201817）；海南省自然科学基金（318MS105）；热带木本油料创新团队项目（1630152017010）；“一带一路”热带项目（BARTP-06）

Isolation and Virulence of Entomogenic Nematodes against Tirathaba rufivena in Areca Plantation of Hainan

ZHONG Baozhu, Lü Chaojun, LI Chaoxu, QIN Weiquan

Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Engineering Research Center of Arecanut Industry, Wenchang 571339, China

Received:2020-09-20 Published:2021-06-16

摘要/Abstract

摘要： 红脉穗螟Tirathaba rufivena Walker是槟榔上的重要害虫。本研究采用大蜡螟诱捕法，对海南省主要槟榔产区槟榔园中采集到的土样进行昆虫病原线虫的诱捕，并评估了目标线虫对红脉穗螟的致病力。在采集的150份土样中共有12个土样经检测发现含有昆虫病原线虫，检出率为8%。其中，屯昌、定安和万宁三个市县采集的土样中检出的带线虫比率相对较高，分别为12.90%、11.76%和10.00%，而海口和保亭土样中未检出昆虫病原线虫。在室内以大蜡螟为试虫测试线虫的致病力，结果表明诱捕的12个线虫种群均对大蜡螟幼虫具有一定的致病力，但致病力的大小存在较大差异（120 h校正死亡率21.11%～72.22%）。选取致病力较高的Tc05、Wn01和Wn09样本测试对红脉穗螟3龄幼虫的致病作用，结果显示在600 IJs/mL线虫液处理后24 h，Wn01种群对寄主的校正死亡率达到86.67%，半致死浓度LC₅₀为283.70 IJs/mL；Tc05和Wn09对红脉穗螟的致病力相近，校正死亡率分别为61.67%和65.00%，LC₅₀分别为504.75 IJs/mL和502.70 IJs/mL。研究表明，Wn01，Tc05和Wn09线虫种群对红脉穗螟幼虫具有一定的控制潜力。

关键词: 昆虫病原线虫, 槟榔园, 红脉穗螟, 致病力

Abstract: Tirathaba rufivena Walker is an important pest of areca palm, Areca catechu L.. In this study, Galleria mellonella bait technology was employed to investigate the distribution of entomopathogenic nematodes(EPN) isolated from soil samples collected in the main betel nut garden of Hainan province, and the virulence of target nematodes to T. rufivena was evaluated. EPNs were found in 12 of 150 collected soil samples with a 8% recovery rate. In Tunchang, Ding'an and Wanning, the recovery rates of nematodes were 12.90%, 11.76% and 10.00%, respectively. No EPN was detected in soil samples from Haikou and Baoting. The results showed that all the 12 EPN populations had certain virulence to the larvae of G. mellonella, but the virulence greatly varied (the corrected mortality ranged from 21.11 to 72.22% after treated 120 h). The virulence of Tc05, Wn01 and Wn09 which have higher pathogenicity to the 3^rd instar larvae of T. rufivena was tested. The results revealed that after treated with 600 IJs/mL EPN suspensions, the corrected mortality rate of Wn01 was 86.67% after 24 h, and the LC₅₀ was 283.70 IJs/mL. Tc05 and Wn09 had similar virulence to the larvae, with corrected mortality of 61.67% and 65.00%, and the LC₅₀ was 504.75 and 502.70 IJs/mL, respectively. The above results indicated that Wn01, Tc05 and Wn09 EPN samples have potential on the control of T. rufivena larvae.

Key words: entomopathogenic nematode, areca plantation, Tirathaba rufivena, virulence

中图分类号:

S476

钟宝珠, 吕朝军, 李朝绪, 覃伟权. 海南槟榔园昆虫病原线虫对红脉穗螟的致病力[J]. 中国生物防治学报, 2021, 37(3): 431-435.

ZHONG Baozhu, Lü Chaojun, LI Chaoxu, QIN Weiquan. Isolation and Virulence of Entomogenic Nematodes against Tirathaba rufivena in Areca Plantation of Hainan[J]. Chinese Journal of Biological Control, 2021, 37(3): 431-435.

参考文献

[1] Georgis R, Koppenh fer A M, Lacey L A, et al. Successes and failures in the use of parasitic nematodes for pest control[J]. Biological Control, 2006, 38:103-123.
[2] 杨怀文, 陈松笔. 昆虫寄生线虫与林业害虫的防治[J]. 林业科学, 1999, 35(6):103-109.
[3] Shapiro D I, Leskey T C, Wright S E. Virulence of entomopathogenic nematodes to plum curculio, Conotrachelus nenuphar:Effects of strain, temperature, and soil type[J]. Journal of Nematology, 2011, 43(3-4):187-195.
[4] Koshel E I, Aleshin V V, Eroshenko G A, et al. Phylogenetic analysis of entomoparasitic nematodes, potential control agents of flea populations in natural foci of plague[J]. Biomed Research International, 2014(1):135218-135243.
[5] Kaya H K, Gaugler R. Entomopathogenic nematodes[J]. Annual Review of Entomology, 1993, 38:389-393.
[6] Grewal P S, Power K T, Grewal S K, et al. Enhanced consistency in biological control of white grubs (Coleoptera:Scarabaeidae) with new strains of entomopathogenic nematodes[J]. Biological Control, 2004, 30:73-82.
[7] Grewal P S, Koppenh fer A M, Choo H Y. Lawn, turfgrass and pasture applications[M]//Grewal P S, Ehlers R-U, Shapiro-Ilan D I, eds. Nematodes as Biocontrol Agents. Wallingford:CABI Publishing, 2005, 115-146.
[8] Yan X, Moens M, Han R C, et al. Effects of selected insecticides on osmotically treated entomopathogenic nematodes[J]. Journal of Plant Disease and Protection, 2012, 119(4):152-158.
[9] 何康来, 周大荣, 杨怀文. 应用芜菁夜蛾线虫防治亚洲玉米螟的研究[J]. 生物防治通报, 1991, 7(1):1-6.
[10] 杨怀文, 张刚应, 张善稿, 等. 芫菁夜蛾线虫对小木蠹蛾侵染能力的研究[J]. 生物防治通报, 1989, 5(3):97-100.
[11] 张中润, 王金辉, 黄海杰, 等. 昆虫病原线虫对腰果云翅斑螟的室内致病力[J]. 植物保护, 2017, 43(1):210-213.
[12] White G F. A method for obtaining infective nematode larvae from cultures[J]. Science, 1927, 66:302-303.
[13] 徐洁莲, 刘秀玲. 温度、储备方法和载体湿度对斯氏线虫存活和感染力的影响[J]. 昆虫天敌, 1997, 19(3):97-102.
[14] 黄剑, 吴文君. 利用EXCEL快速进行毒力测定中的致死中量计算和卡方检验[J]. 昆虫知识, 2004, 41(6):594-598.
[15] Garcia P F, Palomo A. Natural occurrence of entomopathogenic nematodes (Rhabditida:Steinernematidae and Heterorhabditidae) in Spanish soils[J]. Journal of Invertebrate Pathology, 1996, 68(1):84-90.
[16] Stock S P, Pryor B M, Kaya H K. Distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in natural habitats in California, USA[J]. Biodiversity & Conservation, 1999, 8(4):535-549.
[17] 钱秀娟, 谷黎娜, 邢玉芳, 等. 甘肃省昆虫病原线虫区系研究[J]. 草地学报, 2014, 22(3):593-599.
[18] 陈书龙, 李秀花, 史均环. 河北省昆虫病原线虫资源调查[J]. 中国生物防治, 2006, 22(1):40-44.
[19] 谷黎娜. 甘肃省昆虫病原线虫区系及优良品系生物学特性研究[D]. 兰州:甘肃农业大学, 2008.
[20] Bedding R A. Logistics and strategies for introducing entomopathogenic nematode technology into developing countries[M]//Entomopathogenic Nematodes in Biological Control, 1990, 233-246.

海南槟榔园昆虫病原线虫对红脉穗螟的致病力

Isolation and Virulence of Entomogenic Nematodes against Tirathaba rufivena in Areca Plantation of Hainan

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