3株氟咯草酮降解菌株的筛选及其生物降解效果研究

doi:10.16409/j.cnki.2095-039x.2021.06.025

摘要/Abstract

摘要： 为探究土壤中具有降解氟咯草酮能力的菌株。采用富集法和平板稀释法筛选菌株，采用液相色谱－串联质谱法（LC-MS/MS）检测分析各菌株对氟咯草酮的降解率，结合形态学观察、16S rDNA和ITS测序分析对筛选得到的优势降解菌株进行鉴定，并采用室内生测法对各菌株进行安全性评价。结果表明，从土壤中获得3株优势降解菌株FL-M-2、FL-M-3、FL-G-2，经鉴定分别为紫色紫孢菌Purpureocillium lavendulum、小双胞腔菌属Didymellaceae sp.和小链霉菌Streptomyces parvus；在接种量为5%，28℃、180 r/min振荡培养7 d，对1 mg/L的氟咯草酮的降解率分别为39.63%、72.09%和68.80%，其中菌株FL-G-2降解效果最好，培养14 d后降解率达到84.37%；安全性试验表明，3株菌的发酵液对小麦和油菜幼苗的生长均无明显的抑制作用。本研究为进一步解析菌株降解氟咯草酮作用机理提供了研究基础，也为进一步开展吡咯烷酮类除草剂的降解提供了新的菌株资源。

关键词: 氟咯草酮, 微生物降解, 降解效果, 安全性

Abstract: To explore the strains with the ability to degrade flurochloridone in the soil. Enrichment methods and plate dilution methods were used for screening strains, and the degradation rate of each strain for flurochloridone was detected by Liquid Chromatography-Tandem Mass Spectrometry. The strains with dominant degradation were identified by combined with morphological observations, 16S rDNA and ITS sequencing analysis, and the safety evaluation of each strain was determined with laboratory bioassays. The results showed that three strains FL-M-2, FL-M-3, FL-G-2 with a significant degradation rate from soil were identified as Purpureocillium lavendrulum, Didymellaceae sp. and Streptomyces parvus, respectively. Three strains cultured with 180 r/min, inoculation amount 5%, culture temperature at 28 ℃ for 7 days in culture medium have the degradation rate of 39.63%, 72.09% and 68.80% for the concentration of 1 mg/L flurochloridone, respectively. Among them, the strain FL-G-2 has the best degradation effect, and its degradation rate reached 84.37% after 14 days of culture. The fermentation broth of three strains had no obvious inhibitory effect on the growth of wheat and rape seedlings. This research provides a basis for analysis of the mechanism of microorganism degradation of flurochloridone, and also offers new strains resource for further development of the degradation of pyrrolidone herbicides.

Key words: flurochloridone, microbial degradation, degradation effect, safety

中图分类号:

S476

雷向荣, 沈硕, 李玮. 3株氟咯草酮降解菌株的筛选及其生物降解效果研究[J]. 中国生物防治学报, 2021, 37(6): 1231-1240.

LEI Xiangrong, SHEN Shuo, LI Wei. Screening of Three Strains for Degrading Flurochloridone and Their Biodegradation Effects[J]. Chinese Journal of Biological Control, 2021, 37(6): 1231-1240.

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