水稻瘤矮病毒介导的转cry1Ab水稻对褐飞虱种群的影响

doi:10.16409/j.cnki.2095-039x.2022.09.001

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (5): 1055-1066.DOI: 10.16409/j.cnki.2095-039x.2022.09.001

• 转基因生物安全专栏 • 下一篇

水稻瘤矮病毒介导的转cry1Ab水稻对褐飞虱种群的影响

苗鑫^1,2,5,6, 刘煜晓^1,2,5,6, 陈文乐³, 郭齐汤⁴, 尤士骏^1,2,5,6, 林胜^1,2,5,6

1. 福建农林大学植物保护学院/闽台作物有害生物生态防控国家重点实验室, 福州 350002;
2. 福建农林大学海峡两岸特色作物安全生产省部共建协同创新中心, 福州 350002;
3. 三明市植保植检站, 三明 365000;
4. 三明市烟草公司大田分公司, 三明 366100;
5. 福建省农产品安全研究平台, 福州 350002;
6. 害虫绿色防控福建省高校重点实验室, 福州 350002

收稿日期:2022-07-15 出版日期:2022-10-08 发布日期:2022-10-20
通讯作者: 林胜,副教授,E-mail:linsn001@163.com
作者简介:苗鑫,博士研究生,E-mail:619283939@qq.com
基金资助:
福建省自然科学基金(2020J01596);国家科技重大专项转基因生物新品种培育(2016ZX08011001-003);国家自然科学基金(31870520);福建农林大学科技创新专项基金(KFb22014XA)

Effects of RGDV-Infected Transgenic cry1Ab Rice on the Populations of Nilaparvata lugens(Stål)

MIAO Xin^1,2,5,6, LIU Yuxiao^1,2,5,6, CHEN Wenle³, GUO Qitang⁴, YOU Shijun^1,2,5,6, LIN Sheng^1,2,5,6

1. State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross- Strait Crops, Fuzhou 350002, China;
3. Sanming Plant Protection and inspection station, Sanming 365000, China;
4. Datian Branch of Sanming Tobacco Company, Sanming, Fujian 366100, China;
5. Fujian Agricultural Product Safety Research Platform, Fuzhou 350002, China;
6. Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China

Received:2022-07-15 Online:2022-10-08 Published:2022-10-20

摘要/Abstract

摘要： 在人工气候室条件下，以抗虫转cry1Ab水稻（MF86）及其亲本对照非转基因水稻闽恢3301（MH3301）为材料，利用年龄-龄期两性生命表，研究水稻瘤矮病毒介导的转cry1Ab水稻对非靶标害虫褐飞虱种群的影响。研究结果表明，感病水稻对取食MH3301的褐飞虱种群寿命影响不显著，促进了其生殖力；而显著缩短了取食MF86的褐飞虱寿命，降低了其生殖力。水稻在不感病的情况下，取食MF86较取食MH3301的褐飞虱种群可显著延长寿命和提高生殖力；而水稻在感病的情况下，取食MF86较取食MH3301的褐飞虱种群生殖力显著降低。感病的MF86较未感病MF86显著降低褐飞虱的内禀增长率、周限增长率，显著缩短世代平均周期；而感病的MH3301较未感病MH3301对褐飞虱生命表动态参数的影响基本均不显著。由此可见，水稻瘤矮病毒介导的转cry1Ab水稻（MF86）对褐飞虱种群增殖具有显著的负面影响。

关键词: 转基因抗虫水稻, 稻飞虱, 非靶标昆虫, 两性生命表, 生物安全

Abstract: The insect-resistant transgenic cry1Ab rice MF86 and its parental non-transgenic MH3301 rice were tested for the effects of Rice gall dwarf virus(RGDV)-infection of rice on the population of the non-target pest,Nilaparvata lugens (brown planthopper,BPH) under the climate chamber conditions using age-stage two sex life table.It was found that RGDV had no significant effect on the lifespan of BPH population feeding on MH3301,while increased its fecundity;in contrast,RGDV significantly shortened the life span of BPH population feeding on MF86 and reduced its fecundity.Compared with MH3301,healthy MF86 significantly prolonged the longevity and increased the fecundity of BPH population.However,compared with RGDV-infected MH3301,RGDV-infected MF86 significantly decreased the fecundity of BPH population.RGDV-infected MF86 significantly reduced the intrinsic rate of increase,finite rate of increase rate of BPH population,and significantly shortened the generation time;in comparison,RGDV-infected MH3301 had no significant effects on the dynamic parameters of BPH population.The results show that the RGDV-infected transgenic cry1Ab rice (MF86) harbor significantly negative effects on the population proliferation of BPH.

Key words: transgenic insect-resistant rice, rice planthopper, non-target insect, two-sex life table, biosafety

中图分类号:

S188

苗鑫, 刘煜晓, 陈文乐, 郭齐汤, 尤士骏, 林胜. 水稻瘤矮病毒介导的转cry1Ab水稻对褐飞虱种群的影响[J]. 中国生物防治学报, 2022, 38(5): 1055-1066.

MIAO Xin, LIU Yuxiao, CHEN Wenle, GUO Qitang, YOU Shijun, LIN Sheng. Effects of RGDV-Infected Transgenic cry1Ab Rice on the Populations of Nilaparvata lugens(Stål)[J]. Chinese Journal of Biological Control, 2022, 38(5): 1055-1066.

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水稻瘤矮病毒介导的转cry1Ab水稻对褐飞虱种群的影响

Effects of RGDV-Infected Transgenic cry1Ab Rice on the Populations of Nilaparvata lugens(Stål)

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