转cry30Fa1水稻对褐飞虱NlHRP基因表达模式的影响

doi:10.16409/j.cnki.2095-039x.2022.09.002

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (5): 1067-1074.DOI: 10.16409/j.cnki.2095-039x.2022.09.002

转cry30Fa1水稻对褐飞虱NlHRP基因表达模式的影响

普琪雯, 焦慧宇, 区文韬, 刘健丽, 林胜

福建农林大学闽台作物有害生物生态防控国家重点实验室/福建农林大学海峡两岸特色作物安全生产省部共建协同创新中心/福建省农产品安全研究平台/害虫绿色防控福建省高校重点实验室, 福州 350002

收稿日期:2022-08-15 出版日期:2022-10-08 发布日期:2022-10-20
通讯作者: 林胜,副教授,E-mail:linsn001@163.com
作者简介:普棋雯,硕士研究生,E-mail:571086829@qq.com
基金资助:
福建省自然科学基金(2020J01596);国家自然科学基金(31870520);国家科技重大专项转基因生物新品种培育(2016ZX08011001-003);福建农林大学科技创新专项基金(CXZX2020020A);福建农林大学大学生创新创业训练计划(202210389174)

Effects of Transgenic cry30Fa1 Rice on the Expression Pattern of NlHRP in Nilaparvata lugens (Stål)

PU Qiwen, JIAO Huiyu, OU Wentao, LIU Jianli, LIN Sheng

State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/Fujian Agricultural Product Safety Research Platform/Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China

Received:2022-08-15 Online:2022-10-08 Published:2022-10-20

摘要/Abstract

摘要： 转cry30Fa1水稻（KF30-14）会显著控制褐飞虱Nilaparvata lugens (Stål)种群数量增长，但其影响机制尚不清楚。本研究利用相关转录组数据分析筛选获得了一个表达量有显著差异的基因NlHRP，并克隆了该基因。序列分析表明，NlHRP编码了一个富组氨酸蛋白，无保守结构域。通过实时荧光定量PCR检测羽化24、72、96 h的褐飞虱雌虫的NlHRP表达量，结果显示该基因在KF30-14褐飞虱实验种群中的表达量均高于MH86实验种群，且在羽化72 h时，达到显著差异；对羽化后72 h褐飞虱雌虫的头、胸、卵巢、中肠、脂肪体的NlHRP表达量检测发现，NlHRP在褐飞虱的头、胸、中肠表达量较高，其中KF30-14褐飞虱实验种群雌虫的NlHRP在头部表达量显著升高。推测NlHRP可能与褐飞虱适应转cry30Fa1水稻的抗性有关，该研究结果为揭示转cry30Fa1水稻抗褐飞虱的分子机制提供相关基础。

关键词: 稻飞虱, 转基因抗虫水稻, NlHRP, 抗虫机制

Abstract: Previous studies found that the transgenic cry30Fa1 rice (KF30-14) could significantly suppress the population growth of brown planthopper (BPH),Nilaparvata lugens (Stål),while the mechanism remains unclear.In this study,using the transcriptome data analysis,a gene called NlHRP with remarkably different expression levels was found and cloned.The result of sequence analysis showed that the NlHRP encoded a histidine-rich protein without conserved domain.RT-qPCR was used to find that the NlHRP expression levels in female BPHs at 24,72 and 96 h after eclosion were higher in KF30-14 experimental population than those in MH86 experimental population,and particularly at 72 h after eclosion were significantly higher.The expression levels of NlHRP in the head,thorax,ovary,midgut and fat body of BPH at 72 h after eclosion were detected by RT-qPCR.The expression levels of NlHRP in the head,midgut and thorax of female BPHs were the highest.The expression level of NlHRP in the head of female BPHs was dramatically increased in KF30-14 experimental population.The results indicate that NlHRP could be related to the BPH adaptation to the transgenic cry30Fa1 rice,and provides the research foundation for the resistant mechanism of transgenic cry30Fa1 rice against BPHs.

Key words: rice planthopper, transgenic insect-resistant rice, NlHRP, insect-resistant mechanism

中图分类号:

S188

普琪雯, 焦慧宇, 区文韬, 刘健丽, 林胜. 转cry30Fa1水稻对褐飞虱NlHRP基因表达模式的影响[J]. 中国生物防治学报, 2022, 38(5): 1067-1074.

PU Qiwen, JIAO Huiyu, OU Wentao, LIU Jianli, LIN Sheng. Effects of Transgenic cry30Fa1 Rice on the Expression Pattern of NlHRP in Nilaparvata lugens (Stål)[J]. Chinese Journal of Biological Control, 2022, 38(5): 1067-1074.

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转cry30Fa1水稻对褐飞虱NlHRP基因表达模式的影响

Effects of Transgenic cry30Fa1 Rice on the Expression Pattern of NlHRP in Nilaparvata lugens (Stål)

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