植物次生代谢物丁布对苜蓿斑蚜的控制作用

doi:10.16409/j.cnki.2095-039x.2023.01.014

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1143-1147.DOI: 10.16409/j.cnki.2095-039x.2023.01.014

植物次生代谢物丁布对苜蓿斑蚜的控制作用

潘凡, 朱凯辉, 涂雄兵, 张泽华

中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193

收稿日期:2022-06-11 出版日期:2023-10-08 发布日期:2023-10-25
通讯作者: 涂雄兵,研究员,E-mail:tuxiongbing@caas.cn。
作者简介:潘凡,硕士研究生,E-mail:lillianp@163.com。
基金资助:
国家现代农业产业技术体系（CARS-34）

Effects of Plant Secondary Metabolite DIMBOA on Therioaphis trifolii Monel (Homptera: Aphididae)

PAN Fan, ZHU Kaihui, TU Xiongbing, ZHANG Zehua

State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2022-06-11 Online:2023-10-08 Published:2023-10-25

摘要/Abstract

摘要： 为明确植物次生代谢物丁布对苜蓿斑蚜Therioaphis trifolii Monel生长发育和繁殖的影响，利用人工饲喂的方法测定不同浓度丁布对苜蓿斑蚜的控制作用。结果表明，高浓度丁布可以显著降低苜蓿斑蚜存活率、繁殖率和体内蛋白酶活性，与对照相比，苜蓿斑蚜取食800 μg/mL丁布72 h后，成蚜存活率下降了53.06%，繁殖率下降了53.82%，若蚜存活率仅为35.8%，显著低于对照组；不同浓度丁布混合饲料均显著抑制了成蚜体内氨肽酶（APs）、胰凝乳蛋白酶（CTP）、胰蛋白酶（Tr）、总蛋白酶（Pr）活性，在高浓度下最为突出，与对照组相比，苜蓿斑蚜取食含有800 μg/mL丁布72 h后，Aps、CTP、Tr、Pr活力分别降低了61.5%、67%、65.1%、55.9%。表明丁布作为重要的植物次生代谢物，可显著降低苜蓿斑蚜存活率和繁殖率，为植物源农药开发提供理论支撑。

关键词: 植物次生代谢物, 苯并噁嗪, 苜蓿斑蚜

Abstract: The effects of plant secondary metabolite DIMBOA on the growth, development and reproduction of Therioaphis trifolii Monel were investigated by mixingdifferent concentrations of DIMBOA with artificial diet. The results showed that the survival rate, reproductive rate and protease activity of T. trifolii were significantly reduced by high concentration of DIMBOA. When the T. trifolii adults were fed 800 μg/mL DIMBOA for 72 h, compared with the control group, the survival rate was reduced by 53.06% and the reproductive rate, by 53.82%. The survival rate of the T. trifolii larva was only 35.8%, significantly lower than that of the control group. The activities of aminopeptidases (APs), chymotrypsin (CTP), trypsin (Tr), and total protease (Pr) in T. trifolii adults were significantly inhibited by different concentrations of DIMBOA, especially by high concentration of DIMBOA. The activities of Aps, CTP, Tr and Pr were reduced by 61.5%, 67%, 65.1% and 55.9%, respectively, in the insects fed with 800 μg/mL DIMBOA for 72 h. These results indicate that DIMBOA can significantly reduce the survival rate and reproductive rate of T. trifolii, showing a potential for the development of plant-derived pesticides.

Key words: plant secondary metabolites, benzoxazine, Therioaphis trifolii

中图分类号:

S476

潘凡, 朱凯辉, 涂雄兵, 张泽华. 植物次生代谢物丁布对苜蓿斑蚜的控制作用[J]. 中国生物防治学报, 2023, 39(5): 1143-1147.

PAN Fan, ZHU Kaihui, TU Xiongbing, ZHANG Zehua. Effects of Plant Secondary Metabolite DIMBOA on Therioaphis trifolii Monel (Homptera: Aphididae)[J]. Chinese Journal of Biological Control, 2023, 39(5): 1143-1147.

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植物次生代谢物丁布对苜蓿斑蚜的控制作用

Effects of Plant Secondary Metabolite DIMBOA on Therioaphis trifolii Monel (Homptera: Aphididae)

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