大豆胰蛋白酶抑制剂对苜蓿斑蚜的控制作用

doi:10.16409/j.cnki.2095-039x.2018.03.012

中国生物防治学报 ›› 2018, Vol. 34 ›› Issue (3): 348-353.DOI: 10.16409/j.cnki.2095-039x.2018.03.012

大豆胰蛋白酶抑制剂对苜蓿斑蚜的控制作用

赵海龙^1,2, 石洋^1,2, 涂雄兵², 董辉¹, 张泽华²

1. 沈阳农业大学植物保护学院, 沈阳 116021;
2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193

收稿日期:2018-01-10 出版日期:2018-06-08 发布日期:2018-06-14
通讯作者: 董辉,教授,E-mail:biocontrol@163.com;张泽华,研究员,E-mail:zhangzehua@caas.cn
作者简介:赵海龙,硕士研究生,E-mail:zhao.hailong@live.com
基金资助:
现代农业产业技术体系（CARS-34-07B）

Effects of Soybean Trypsin Inhibitors on Therioaphis trifolii Monel (Homptera: Aphididae)

ZHAO Hailong^1,2, SHI Yang^1,2, TU Xiongbing², DONG Hui¹, ZHANG Zehua²

1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Academy of Agricultural Sciences, Beijing 100193, China

Received:2018-01-10 Online:2018-06-08 Published:2018-06-14

摘要/Abstract

摘要： 苜蓿斑蚜是危害苜蓿的重要害虫，利用生物制剂防治苜蓿斑蚜能有效控制其持续为害。本文利用人工饲喂方法，测定两种大豆胰蛋白酶抑制剂（KTI和BBI）对苜蓿斑蚜的控制作用。结果表明：经浓度均为800 μg/mL的KTI和BBI处理后，随处理时间延长，成蚜存活率降低，与对照相比，处理72 h后存活率分别降低了38.3%、41.7%，繁殖率分别降低了128.3%、116.7%，第2代若蚜存活率分别降低了6.7%、19.8%。上述结果表明大豆胰蛋白酶抑制剂能有效降低苜蓿斑蚜虫口密度，该结果为进一步研制和应用生物制剂防治苜蓿斑蚜提供了重要的理论依据。

关键词: 胰蛋白酶抑制剂, 苜蓿斑蚜, 生物农药, 存活率, 繁殖率

Abstract: Spotted alfalfa aphid, Therioaphis trifolii Monel, causes huge damages of alfalfa. Application of biological agents can ensure a sustainable management. In this paper, two different soybean trypsin inhibitors (KTI and BBI) were tested for their inhibition effects on spotted alfalfa aphid using artificial feeding method. The results showed that the survival rate of adult aphids decreased with the time post feeding of KTI and BBI (800 μg/mL). In comparison with the control, 72 hours after feeding of KTI and BBI, the survival rate of adult aphids were reduced by 38.3% and 41.7%, respectively, the reproduction rate, by 128.3% and 116.7%, respectively, and the survival rate of the larval aphids, by 6.7% and 19.7%, respectively. These results indicate that the soybean trypsin inhibitors KTI and BBI can effectively reduce the population density of the spotted alfalfa aphid.

Key words: trypsin inhibitor, spotted alfalfa aphid, biological pesticide, survival rate, reproduction rate

中图分类号:

S476

赵海龙, 石洋, 涂雄兵, 董辉, 张泽华. 大豆胰蛋白酶抑制剂对苜蓿斑蚜的控制作用[J]. 中国生物防治学报, 2018, 34(3): 348-353.

ZHAO Hailong, SHI Yang, TU Xiongbing, DONG Hui, ZHANG Zehua. Effects of Soybean Trypsin Inhibitors on Therioaphis trifolii Monel (Homptera: Aphididae)[J]. journal1, 2018, 34(3): 348-353.

参考文献

[1] 吴志刚, 朱蒙猛, 张蓉, 等. 苜蓿斑蚜对我国苜蓿商品草的经济损失评估[J]. 宁夏农林科技, 2013, 54(11):74-77.
[2] 唐平华, 陈国平, 朱明库, 等. 蚜虫防治技术研究进展[J]. 植物保护, 2013, 39(2):5-12, 19.
[3] 邱德文. 我国生物农药现状分析与发展趋势[J]. 植物保护, 2007, 33(1):27-32.
[4] Fan L M. Research on the development of botanical pesticide based on analysis of scientific materials[J]. Advanced Materials Research, 2011, (282-283):509-513.
[5] Ryan C A. Protease inhibitors in plants:genes for improving defenses against insects and pathogens[J]. Annual Review of Phytopathology, 1990, 28(1):425-449.
[6] Hilder V A, Gatehouse A M R, Sheerman S E, et al. A novel mechanism of insect resistance engineered into tobacco[J]. Nature, 1987, 330(6144):160-163.
[7] Chikhale R S D N J. Plant protease inhibitors:Strategy for pest control in crops[J]. Lifeences Leaflets, 2014, 58:976-1098.
[8] Rawlings N D, Tolle D P, Barrett A J. Evolutionary families of peptidase inhibitors[J]. Biochemical Journal, 2004, 378(3):705-716.
[9] Houseman J G A M, Larocqueand N M R. The insect protease, plant protease inhibitors, and possible pest control[J]. Memoirs of the Entomological Society of Canada, 1991, 123(159):3-11.
[10] Franco O L, Dias S C, Magalhaes C P, et al. Effects of soybean Kunitz trypsin inhibitor on the cotton boll weevil (Anthonomns grandis)[J]. Phytochemistry, 2004, 65(1):81-89.
[11] Chougule N P, Doyle E, Fitches E, et al. Biochemical characterization of midgut digestive proteases from Mamestra brassicae (Lepidoptera:Noctuidae) and effect of soybean Kunitz inhibitor (SKTI) in feeding assays[J]. Journal of Insect Physiology, 2008, 54(3):563-572.
[12] Ryan C A. Protease inhibitors in plants:genes for improving defenses against insects and pathogens[J]. Annual Review of Phytopathology, 1990, 28(1):425-449.
[13] Macedo M L R, Freire M G M. Insect digestive enzymes as a target for pest control[J]. Invertebrate Survival Journal, 2011, 8(2):190-198.
[14] 高文兴, 王孟卿, 陈红印. 蚜虫人工饲料的研究及应用进展[J]. 中国生物防治学报, 2012, 28(1):121-127.
[15] Mittler T E, Dadd R H. Studies on the artificial feeding of the aphid Myzus persicae (Sulzer)-I[J]. Journal of Insect Physiology, 1963, 9(5):623-645.
[16] Auclair J L, Cartier J J. Pea aphid:rearing on a chemically defined diet[J]. Science, 1963, 142(3595):1068.
[17] Cress D C, Chada H L. Development on two synthetic diets for the greenbug, Schizaphisgraminum[J]. Annals of the Entomological Society of America, 1971, 64(6):1237-1240.
[18] Mittler T E, Dadd R H. Studies of relative uptake of water and sucrose solutions on the artificial feeding of the aphid Myzus persicae (Sulzer)[J]. Journal of Insect Physiology, 1963, 9(5):623-645.
[19] Jacques C J, Auclair J L. Pea aphid behaviour:colour preference on a chemical diet[J]. Canadian Entomologist, 1964, 96(9):1240-1243.
[20] Tahori A S, Hazan A. Rearing of the black citrus aphid Toxoptera aurantii on chemically defined diets[J]. Journal of Insect Physiology, 1970, 16(10):1975-1981.
[21] Akey D H, Beck S D. Nutrition of the pea aphid, Acyrthosiphon pisum:requirements for trace metals, sulphur, and cholesterol[J]. Journal of Insect Physiology, 1972, 18(10):1901-1914.
[22] Kunkel H, Harris K F, Maramorosch K, et al. Aphis as Virus Vectors[M]. New York:Academic Press, 1977, 311-318.
[23] Mittler T E. Studies on the feeding and nutrition of Tuberolachnus salignus (Gmelin) (Homoptera, Aphididae)[J]. Journal of Experimental Biology, 1958, 35(1):74-84.
[24] Emden H F V. An Increase in the longevity of adult aphis fabae, fed artificially through parafilm membranes on liquids under pressure[J]. Entomologia Experimentalis et Applicata, 1967, 10(2):166-170.
[25] Nalbant D. Water stress and Russian wheat aphid interactions in wheat[J]. Texas Tech University, 1995(12):182.
[26] Mariateresa V, Luigi R C, Raffaele G, et al. Functional expression on bacteriophage of the mustard trypsin inhibitor MTI-2[J]. Biochemical and Biophysical Research Communications, 2001, 280(3):813-817.
[27] Chen D Y, Chen F Y, Hong G J, et al. Jasmonate response decay and defense metabolite accumulation contributes to age-regulated dynamics of plant insect resistance[J]. Nature Communications, 2017, 8(10):13925.
[28] 黄伟. 不同紫花苜蓿品种抗蚜性鉴定及抗性机理初步研究[D]. 杨凌:西北农林科技大学, 2007.
[29] Mauch-Mani B, Baccelli I, Luna E, et al. Defense priming:an adaptive part of induced resistance[J]. Annual Review of Plant Biology, 2017, 68(1):485.
[30] Tatsuta K, Mikami N, Fujimoto K, et al. The structure of chymostatin, a chymotrypsin inhibitor[J]. Journal of Antibiotics, 1973, 26(11):625-646.
[31] Roychaudhuri R, Sarath G, Zeece M, et al. Reversible denaturation of the soybean Kunitz trypsin inhibitor[J]. Archives of Biochemistry and Biophysics, 2003, 412(1):20.
[32] Scarafoni A, Consonni A, Galbusera V, et al. Identification and characterization of a Bowman-Birk inhibitor active towards trypsin but not chymotrypsin in Lupinus albus seeds[J]. Phytochemistry, 2008, 69(9):1820.
[33] Schneider F, Houseman J G, Morrison P E. Activity cycles and the regulation of digestive proteases in the posterior midgut of Stomoxys calcitrans[J]. Insect Biochemistry, 1987, 17(6):859-862.
[34] Chougule N P, Doyle E, Fitches E, et al. Biochemical characterization of midgut digestive proteases from Mamestra brassicae and effect of soybean Kunitz inhibitor (SKTI) in feeding assays[J]. Journal of Insect Physiology, 2008, 54(3):563-572.
[35] Broadway R M, Duffey S S. Plant proteinase inhibitors:Mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea, and Spodoptera exiqua[J]. Journal of Insect Physiology, 1986, 32(10):827-833.
[36] 王琛柱, 项秀芬, 张书芳, 等. 大豆胰蛋白酶抑制剂对棉铃虫幼虫消化生理和生长发育的影响[J]. 昆虫学报, 1995, 38(3):272-277.
[37] 徐林波. 苜蓿斑蚜实验种群生命表研究[J]. 中国植保导刊, 2013, 33(3):5-7, 11.

大豆胰蛋白酶抑制剂对苜蓿斑蚜的控制作用

Effects of Soybean Trypsin Inhibitors on Therioaphis trifolii Monel (Homptera: Aphididae)

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