基于非靶标代谢组学分析拜赖青霉菌株47M-1的抑菌活性成分

doi:10.16409/j.cnki.2095-039x.2023.02.055

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1156-1171.DOI: 10.16409/j.cnki.2095-039x.2023.02.055

基于非靶标代谢组学分析拜赖青霉菌株47M-1的抑菌活性成分

赵新贝, 倪云霞, 刘新涛, 赵辉, 闫文庆, 何碧珀, 刘红彦

河南省农业科学院植物保护研究所/农业农村部华北南部作物有害生物综合治理重点实验室/河南省农作物病虫害防治重点实验室/河南省生物农药工程研究中心/河南省作物保护国际联合实验室/河南省农用微生物创新中心, 郑州 450002

收稿日期:2023-03-28 出版日期:2023-10-08 发布日期:2023-10-25
通讯作者: 倪云霞,女,副研究员,E-mail:nyx2008@163.com;刘红彦,男,博士,研究员,E-mail:liuhy1219@163.com。
作者简介:赵新贝,女,博士,助理研究员,E-mail:zhaoxinbei121@163.com。
基金资助:
河南省农科院优秀青年科技基金（2022YQ04）；河南省博士后经费资助；财政部和农业农村部国家现代农业产业技术体系（CARS-14）；河南省重点研发专项（221111520400）；河南省科技攻关项目（222102110251）；河南省科技攻关项目（222102110277）

Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics

ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan

Institute of Plant Protection, Henan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Ministry of Agriculture and Rural Affairs/Henan Key Laboratory of Crop Pest Control/Biological Pesticides Engineering Research Center of Henan Province/International Joint Research Laboratory for Crop Protection of Henan/Agricultural Microbiology Innovation Center of Henan Province, Zhengzhou 450002, China

Received:2023-03-28 Online:2023-10-08 Published:2023-10-25

摘要/Abstract

摘要： 拜赖青霉Penicillium bilaiae菌株47M-1对多种芝麻病原真菌具有显著的抑制作用，为了分析该菌株产生的抑菌活性物质，本研究使用液相色谱串联质谱（LC-MS/MS）技术，检测菌株47M-1培养48、96和144 h时的代谢物，并分析上调差异代谢物在HMDB数据库的分类情况和KEGG代谢通路富集情况，进一步锁定潜在的活性代谢物，进行聚类分析，并验证部分上调代谢物的抑菌活性。经代谢组学数据分析，筛选出105种上调差异代谢物，上调差异代谢物在苯丙素和聚酮类化合物超类中富集最多。KEGG代谢通路富集分析结果表明，上调差异代谢物主要富集在代谢途径、辅因子生物合成、嘌呤代谢、氨基酸生物合成和ABC转运体等通路。通过含药平板法对部分上调差异代谢物的抑菌活性进行验证，其中柚皮素、原儿茶酸、D-(-)-奎宁酸、橙皮素、3-(3-羟基苯基)丙酸、3, 4-二羟苯基丙酸、4’, 5, 7-三羟黄烷酮、迷迭香酸、佛手苷内酯和圣草酚，对芝麻病原真菌具有良好的抑菌活性。本研究明确了菌株47M-1产生的潜在抑菌活性代谢物的主要类群和关键代谢通路，并验证了部分上调差异代谢物的抑菌活性，为菌株47M-1的进一步开发利用及微生物源农药的研发提供了理论支持。

关键词: 拜赖青霉, 代谢组, 抑菌活性物质, 苯丙素和聚酮类, 代谢途径

Abstract: Strain 47M-1 of Penicillium bilaiae showed significant inhibitory effect on a variety of sesame pathogenic fungi. In order to analyze the inhibitiory active substances produced by this strain, liquid chromatography tandem mass spectrometry was used to detect the metabolites of strain cultured for 48 h, 96 h and 144 h. Then, classification of up-regulated differential metabolites in the HMDB database and enrichment of KEGG metabolic pathways were analyzed in order to identify the active metabolites. A total of 105 up-regulated differential metabolites were detected, which were mainly enriched in the superclasses of phenylpropanoids and polyketides. The enrichment analysis of the KEGG metabolic pathways showed that the up-regulated differential metabolites were mainly enriched in metabolic pathways, biosynthesis of cofactors, purine metabolism, biosynthesis of amino acids and ABC transporters. The inhibitiory activities of some up-regulated differential metabolites were verified using medicated plate method. These compounds naringenin, protocatechuic acid, D-(-)-quinic acid, hesperetin, 3-(3-Hydroxyphenyl) propanoic acid, 3, 4-dihydroxyhydrocinnamic acid, 5, 7-dihydroxy-2-(4-hydroxyphenyl)-3, 4-dihydro-2H-1-benzopyran-4-one, rosmarinic acid, bergapten and eriodictyol all showed excellent inhibition activities against sesame pathogenic fungi. In this study, the major groups and key metabolic pathways of potential inhibition metabolites produced by strain 47M-1 were identified, and the inhibitiory activity of some up-regulated differential metabolites was verified. This study will provide data support for the further development and utilization of strain 47M-1 and the research and development of microbial pesticide.

Key words: Penicillium bilaiae, metabolome, inhibition active substances, phenylpropanoids and polyketides, metabolic pathways

中图分类号:

S476

赵新贝, 倪云霞, 刘新涛, 赵辉, 闫文庆, 何碧珀, 刘红彦. 基于非靶标代谢组学分析拜赖青霉菌株47M-1的抑菌活性成分[J]. 中国生物防治学报, 2023, 39(5): 1156-1171.

ZHAO Xinbei, NI Yunxia, LIU Xintao, ZHAO Hui, YAN Wenqing, HE Bipo, LIU Hongyan. Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics[J]. Chinese Journal of Biological Control, 2023, 39(5): 1156-1171.

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基于非靶标代谢组学分析拜赖青霉菌株47M-1的抑菌活性成分

Analysis of Inhibitiory Active Substances of Penicillium bilaiae Strain 47M-1 Based on Non-Targeted Metabolomics

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