3种植物源化合物对亚洲小车蝗的生物活性及影响机制

doi:10.16409/j.cnki.2095-039x.2024.01.011

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (2): 497-506.DOI: 10.16409/j.cnki.2095-039x.2024.01.011

• 研究简报 • 上一篇

3种植物源化合物对亚洲小车蝗的生物活性及影响机制

康鹏¹, 王贻卉¹, 常静¹, 马崇勇², 王建³, 贾雪峰³, 李海平¹

1. 内蒙古农业大学园艺与植物保护学院, 呼和浩特 010019;
2. 内蒙古自治区林业和草原有害生物防治检疫总站, 呼和浩特 010020;
3. 内蒙古自治区乌拉特前旗林业和草原局草原工作站, 乌拉特前旗 014400

收稿日期:2022-09-04 发布日期:2024-04-28
通讯作者: 李海平,博士,教授。E-mail:lihaiping5820@hotmail.com。
作者简介:康鹏,硕士,E-mail:841827817@qq.com。
基金资助:
内蒙古自治区林业和草原有害生物防治检疫总站项目资助

Biological Activity and Influence Mechanism of Three Plant Derived Compounds on Oedaleus decorus asiaticus

KANG Peng¹, WANG Yihui¹, CHANG Jing¹, MA Chongyong², WANG Jian³, JIA Xuefeng³, LI Haiping¹

1. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China;
2. Forestry and Grassland Pest Control and Quarantine Station of Inner Mongolia Autonomous Region, Hohhot 010020, China;
3. Urad Front Banner Grassland Station of Forestry and Grassland Bureau, Inner Mongolia Autonomous Region, Urad Front Banner 014400, China

Received:2022-09-04 Published:2024-04-28

摘要/Abstract

摘要： 亚洲小车蝗是草原上重要的害虫之一，本文研究了3种植物源化合物瑞香狼毒根石油醚提取物、百里香酚和印楝素对亚洲小车蝗3龄蝗蝻的生物活性及影响机制。结果表明，3种植物源化合物对亚洲小车蝗的毒性表现为：拒食作用＞胃毒作用＞触杀作用。48 h时瑞香狼毒根石油醚提取物、百里香酚和印楝素对亚洲小车蝗3龄蝗蝻拒食活性的拒食中浓度（ AFC₅₀）分别为433.97 mg/L、183.35 mg/L和86.47 mg/L，胃毒活性的致死中浓度（ LC₅₀）分别为7976.33 mg/L、269.65 mg/L、341.07 mg/L，触杀作用相对较小。3种植物源化合物对亚洲小车蝗3龄蝗蝻离体腺苷三磷酸酶（ ATPase）和乙酰胆碱酯酶（ AChE）活性均有抑制作用，且随着药剂浓度增大抑制作用增强，抑制率不超过50%。3种化合物处理亚洲小车蝗3龄蝗蝻48 h后，亚洲小车蝗体内谷胱甘肽-S-转移酶（ GSTs）和细胞色素P450活性升高，羧酸酯酶（ CarE）活性受到抑制。研究结果表明，这3种植物源化合物对亚洲小车蝗都具有一定的杀灭作用，拒食作用最强，未来在亚洲小车蝗的防控上可进一步开发利用。

关键词: 瑞香狼毒, 百里香, 印楝素, 亚洲小车蝗

Abstract: Oedaleus decorus asiaticus is one of the important pests on grassland.The mechanism and the biological activity of the petroleum extract from Stellera.chamaejasme roots,azadirachtin and thymol to the third instar of O.decorus asiaticus were measured in this paper.The results showed that the toxicity of the three plant derived compounds to O.decorus asiaticus was antifeedant activity＞stomach activity＞contact activity.The median antifeedant concentration (AFC₅₀) of petroleum ether extract from S.chamaejasme root,thymol and azadirachtin on the third instar of O.decorus asiaticus was 433.97 mg/L,183.35 mg/L and 86.47 mg/L respectively,and the median lethal concentration (LC₅₀) of stomach activity was 7976.33 mg/L,269.65 mg/L and 341.07 mg/L respectively at 48 h.The contact activities of the three plant derived compounds were small relatively.The activities of ATPase and AChE in vitro of the third instar of O.decorus asiaticus were inhibited by the three plant derived compounds,and the inhibition increased with the increasing of the concentrations,but the inhibition rate was not more than 50%.The activity of GSTs and cytochrome P450 of the third instar of O.decorus asiaticus increased,but the activity of CarE was inhibited after treatment of 48 h.The results showed that O.decorus asiaticus could be controlled by three plant derived compounds,and antifeedant activity was the most.The three plant derived compounds can be developed in controling O.decorus asiaticus in the future.

Key words: Stellera chamaejasme, Thymus mongolicus, azadirachtin, Oedaleus decorus asiaticus

中图分类号:

S476

康鹏, 王贻卉, 常静, 马崇勇, 王建, 贾雪峰, 李海平. 3种植物源化合物对亚洲小车蝗的生物活性及影响机制[J]. 中国生物防治学报, 2024, 40(2): 497-506.

KANG Peng, WANG Yihui, CHANG Jing, MA Chongyong, WANG Jian, JIA Xuefeng, LI Haiping. Biological Activity and Influence Mechanism of Three Plant Derived Compounds on Oedaleus decorus asiaticus[J]. Chinese Journal of Biological Control, 2024, 40(2): 497-506.

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3种植物源化合物对亚洲小车蝗的生物活性及影响机制

Biological Activity and Influence Mechanism of Three Plant Derived Compounds on Oedaleus decorus asiaticus

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