印楝素抑制基因转录导致棉铃虫幼虫蜕皮变态异常的分子机理

doi:10.16409/j.cnki.2095-039x.2022.03.015

摘要/Abstract

摘要： 印楝素是一种对鳞翅目如棉铃虫Helicoverpa armigera有良好防治效果的植物源农药。本研究采用室内毒力测定方法，荧光定量PCR（qRT-PCR）和代谢产物差异分析等方法，分析了饲喂印楝素后导致棉铃虫幼虫死亡的分子机理。首先统计分析了5龄和6龄棉铃虫幼虫饲喂不同浓度的印楝素后的表型、体质量、死亡率和化蛹率，结果表明印楝素导致棉铃虫幼虫体质量降低、死亡率升高和化蛹率降低。随后，通过qRT-PCR、酶活和内源物质含量测定，明确了印楝素抑制海藻糖的生物合成和分解，抑制20E下游基因转录表达，并降低了ATP含量。本研究结果表明印楝素通过抑制棉铃虫海藻糖的生物合成和分解，抑制20E信号通路基因转录，降低ATP产量，导致幼虫蜕皮变态失败，最终导致幼虫死亡。本研究对进一步探索印楝素导致棉铃虫死亡的机制和防治有害生物具有重要意义。

关键词: 棉铃虫, 印楝素, 海藻糖酶, ATP合酶α亚基, 20E通路基因

Abstract: Azadirachtin is a botanical pesticide with good control effect on Lepidoptera, such as Helicoverpa armigera. In this study, the molecular mechanism of the death of H. armigera larvae after feeding with Azadirachtin was analyzed by the laboratory virulence assay, real-time quantitative PCR (qRT-PCR), and metabolite measurement. Firstly, the phenotype, body weight, mortality and pupation rate of the 5th and 6th instar larvae which were fed with different concentrations of Azadirachtin were analyzed. The results showed that Azadirachtin resulted in decreased body weight, increased mortality and decreased pupation rate of H. armigera larvae. Furthermore, we proved that Azadirachtin inhibiting the biosynthesis and hydrolysis of trehalose, the transcripts of 20E downstream genes, and reducing the ATP content through by qRT-PCR, enzyme activity and endogenous substance contents measurement. The results of this study indicate that Azadirachtin inhibited the biosynthesis and metabolism of trehalose, decreased the transcripts of 20E pathway genes, and reduced ATP production in H. armigera, so that causing molting and metamorphosis failure, and eventually led to the death of the larvae. This study is of great significance for further exploring the mechanism of Azadirachtin-induced death in H.armigera and control of pests by Azadirachtin.

Key words: Helicoverpa armigera, azadirachtin, trehalase, ATP, 20E pathway genes

中图分类号:

S476

常彦鹏, 张博, 耿梓宸, 魏纪珍, 安世恒, 赵文丽. 印楝素抑制基因转录导致棉铃虫幼虫蜕皮变态异常的分子机理[J]. 中国生物防治学报, 2023, 39(3): 550-559.

CHANG Yanpeng, ZHANG Bo, GENG Zichen, WEI Jizhen, AN Shiheng, ZHAO Wenli. Molecular Mechanism of Azadirachtin Inhibiting Trehalase Activity Leading to Abnormal Molting and Metamorphosis of Helicoverpa armigera Larvae[J]. Chinese Journal of Biological Control, 2023, 39(3): 550-559.

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