非脱羧勒克菌ZF523的分离鉴定及其对植物促生能力研究

doi:10.16409/j.cnki.2095-039x.2025.02.042

中国生物防治学报 ›› 2025, Vol. 41 ›› Issue (4): 949-958.DOI: 10.16409/j.cnki.2095-039x.2025.02.042

• 研究论文 • 上一篇

非脱羧勒克菌ZF523的分离鉴定及其对植物促生能力研究

刘阳^1,2, 王巧^1,3, 谢学文¹, 石延霞¹, 柴阿丽¹, 范腾飞¹, 李宝聚¹, 李磊¹

1. 中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室, 北京 100081;
2. 甘肃农业大学, 兰州 730000;
3. 北京农学院, 北京 102206

收稿日期:2024-12-25 发布日期:2025-08-15
通讯作者: 李磊, 李宝聚
作者简介:刘阳，女，博士研究生，E-mail： liuy2723@163.com；王巧，女，硕士研究生，E-mail： wq1196168805@163.com；通信作者，李磊，男，博士，研究员，E-mail： lilei01@caas.cn；李宝聚，男，博士，研究员，E-mail： libaojuivf@163.com。
基金资助:
国家重点研发计划（2023YFD1401200）；中国农业科学院科技创新工程项目（CAAS-ASTIP-IVFCAAS）；农业农村部园艺作物生物学与种质创制重点实验室（IVF2023）；蔬菜生物育种全国重点实验室开放课题

Isolation and Identification of Leclercia adecarboxylata and Its Effect on Promote Plant Growth

LIU Yang^1,2, WANG Qiao^1,3, XIE Xuewen¹, SHI Yanxia¹, CHAI Ali¹, FAN Tengfei¹, LI Baoju¹, LI Lei¹

1. State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Gansu Agricultural University, Lanzhou 730000, China;
3. Beijing University of Agriculture, Beijing 102206, China

Received:2024-12-25 Published:2025-08-15

摘要/Abstract

摘要： 通过筛选高效促生菌株，分析菌株促生应用潜力，为微生物菌剂的研发提供优良菌种资源。本研究采用Salkowski比色法筛选土壤中具有产吲哚乙酸（indole-3-acetic acid，IAA）能力的微生物，并定量复筛高产IAA的菌株。采用形态学、生理生化特征及16S rDNA基因序列构建系统进化树对促生菌进行鉴定，分析菌株在固氮、产嗜铁素、溶磷和解钾能力等方面的促生潜力。进一步通过盆栽和田间试验来探究菌株的促生效果。结果表明，经筛选获得15株具有较高合成IAA能力菌株，其中ZF523的IAA产量最高（98.71 μg/mL）。根据形态特征、生理生化、16S rRNA基因序列分析将ZF523鉴定为非脱羧勒克菌Leclercia adecarboxylata，其具有较强的固氮、产嗜铁素、溶磷和解钾能力。非脱羧勒克菌ZF523灌根处理显著增加黄瓜、玉米和小麦幼苗的株高和根长。盆栽试验显示，与清水对照（CK）相比，非脱羧勒克菌ZF523处理白菜株高和鲜重增加25.98%和40.96%，对黄瓜的株高和鲜重的增长率达17.01%和16.12%。田间试验表明，非脱羧勒克菌处理可有效提高乌塌菜、苦苣、奶油生菜、芹菜和娃娃菜的株高和鲜重等生长指标。表明菌株ZF523具有促进植物生长的潜力，是良好的促生菌生物资源，开发前景广阔。

关键词: 非脱羧勒克菌, 分子生物学鉴定, 促生特性, 田间试验

Abstract: The highly efficient growth-promoting strains were screened and their potential was analyzed to provide excellent strain resources for the research and development of microbial agents. In this study, Salkowski assay was used to select the microorganisms capable to produce indole-3-acetic acid (IAA) in soil and quantitatively screen high-yielding IAA-producing strains. Morphological, physiological and biochemical characteristics, combined with the phylogenetic analysis based on 16S rDNA gene sequences, were employed to identify the strain. The growth-promoting potential of the strains was evaluated based on its capabilities in nitrogen fixation, siderophore production, dissolve phosphorus and potassium releasing. Furthermore, the growth-promoting effect of the strain was detected by pot and field experiments. The results demonstrated that 15 strains with a high capacity for IAA synthesis were obtained, among which ZF523 exhibited the highest IAA production (98.71 μg/mL). and was identified as Leclercia adecarboxylata, furthermore, it has the abilities of nitrogen fixation, siderophore production, phosphate solubilization and potassium releasing. The inoculating the suspension of Leclercia adecarboxylata ZF523 could effectively improve the plant height and root length of cucumber, maize and wheat seedlings. Compared with the water control (CK), the plant height and biomass of Chinese cabbage, cucumber treated with ZF523 increased by 25.98% and 40.96%, 17.01% and 16.12%, respectively. Result of field experiments indicated that the plant height and fresh weight of savoy, Sonchus oleraceus, butterhead lettuce, celery and mini Chinese cabbage were effectively increased by Leclercia adecarboxylata treatment. It suggested that strain ZF523 has the potential to promote plant growth and serves as an excellent broad-spectrum biological resource for growthpromoting bacteria.

Key words: Leclercia adecarboxylata, molecular biological identification, gowth promoting property, field trials

中图分类号:

S476

刘阳, 王巧, 谢学文, 石延霞, 柴阿丽, 范腾飞, 李宝聚, 李磊. 非脱羧勒克菌ZF523的分离鉴定及其对植物促生能力研究[J]. 中国生物防治学报, 2025, 41(4): 949-958.

LIU Yang, WANG Qiao, XIE Xuewen, SHI Yanxia, CHAI Ali, FAN Tengfei, LI Baoju, LI Lei. Isolation and Identification of Leclercia adecarboxylata and Its Effect on Promote Plant Growth[J]. Chinese Journal of Biological Control, 2025, 41(4): 949-958.

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非脱羧勒克菌ZF523的分离鉴定及其对植物促生能力研究

Isolation and Identification of Leclercia adecarboxylata and Its Effect on Promote Plant Growth

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