重组增效蛋白对Bt 和氯氰菊酯防治棉铃虫的增效作用

重组增效蛋白对Bt 和氯氰菊酯防治棉铃虫的增效作用

袁哲明;陈浩涛;梁晨彩

湖南农业大学生物安全科技学院, 长沙　410128

收稿日期:2005-10-18 修回日期:1900-01-01 出版日期:2006-08-08 发布日期:2006-08-08
通讯作者: 袁哲明

Synergism of Recombinant Enhancin to Bacillus thuringiensis and Cypermethrin against the Larvae of Helicoverpa armigera

YANG Zhe-ming , CHEN Hao-tao , LIANG Chen-cai

Bio-safety Science and Technology College , Hunan Agricultural University , Changsha 410128 , China

Received:2005-10-18 Revised:1900-01-01 Online:2006-08-08 Published:2006-08-08
Contact: YANG Zhe-ming

摘要/Abstract

摘要： 前期在大肠杆菌中表达得到具有显著生物活性的含粉纹夜蛾颗粒体病毒增效蛋白C 端818 个氨基酸的融合蛋白P96。以毒饲料法研究P96 对苏云金芽孢杆菌和氯氰菊酯杀棉铃虫敏感、抗性品系的增效作用。结果表明: P96 对Bt 杀棉铃虫敏感、低抗、中抗品系的增效比分别为.34、4.72 和9.82 ; 对氯氰菊酯杀棉铃虫敏感、低抗、中抗品系的增效比分别为2.53、3.38和6.24。随棉铃虫抗性升高,P96 的增效作用愈为明显。单对汰选是快速选育棉铃虫抗Bt 品系的有效方法。

Abstract: A recombinant enhancin from Trichoplusia ni granulovirus had been expressed successfully in Escherichia coli and named as P96 in the previous study. In this paper , a series bioassay was conducted to characterize the synergy of P96 to Bacillus thuringiensis (Bt) and cypermethrin against the larvae of Helicoverpa armigera. The results indicated that P96 increased the toxicity of Bt and cypermethrin markedly to their susceptible , low-resistant and mid-resistant strains of H. armigera , the synergy ratio were 3.34 , 4.72 , 9.82 for Bt and 2.53 , 3.38 , 6.24 for cypermethrin , respectively. With the increase of resistance ratio , the synergy ratio of P96 also increased. Single-pair selection is an effective approach to speed up insect resistance in laboratory.

袁哲明;陈浩涛;梁晨彩. 重组增效蛋白对Bt 和氯氰菊酯防治棉铃虫的增效作用[J]. , DOI: .

YANG Zhe-ming , CHEN Hao-tao , LIANG Chen-cai. Synergism of Recombinant Enhancin to Bacillus thuringiensis and Cypermethrin against the Larvae of Helicoverpa armigera[J]. , DOI: .