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长柄木霉ACCC30150与哈茨木霉ACCC30371产厚垣孢子的液体培养条件

顾金刚;律雪燕;胡丹丹;李世贵;姜瑞波   

  1. 中国农业科学院农业资源与农业区划研究所/中国农业微生物菌种保藏管理中心, 北京 100081
  • 收稿日期:2007-01-13 修回日期:1900-01-01 出版日期:2008-08-08 发布日期:2008-08-08

Production of Chlamydospores of Trichoderma longbrachiatum ACCC30150 and Trichoderma harzianum ACCC30371 by Liquid Fermentation

GU Jin-gang;LV Xue-yan;HU Dan-dan;LI Shi-gui;JIANG Rui-bo   

  1. Institute of Agricultural Resources and Planning, Agricultural Culture Collection of China ACCC, CAAS, Beijing 100081, China
  • Received:2007-01-13 Revised:1900-01-01 Online:2008-08-08 Published:2008-08-08

摘要: 对长柄木霉ACCC30150与哈茨木霉ACCC30371产生厚垣孢子的液体培养条件进行了研究。结果表明,培养基、培养温度、初始pH值、氧气等因素对两菌株产生厚垣孢子的数量均有影响。在培养基、培养温度、初始pH值不变的培养条件下,装瓶量对两菌株产生厚垣孢子数量的影响大于转速。两菌株产生厚垣孢子的最佳条件: 长柄木霉ACCC30150为玉米秸秆粉或Gorodkowa培养基,在28℃、初始pH5.0、250r/min、装瓶量75ml/250ml下培养,10d后厚垣孢子数量达4.07×108个/ml; 哈茨木霉ACCC30371为玉米秸秆粉培养基,在28℃、初始pH6.0、250r/min、装瓶量75ml/250ml下培养,10d后厚垣孢子数量达5.13×108个/ml。

Abstract: Culture medium, temperature, pH and content of oxygen can affect the production of chlamydospores of Trichoderma longbrachiatum ACCC30150 and T. harziamum ACCC30371. Our results indicated that the best single-factor conditions for the production of chlamydospores of T. longbrachiatum ACCC30150 were as follow: straw powder or Gorodkowa as medium, 28℃, pH5.0, 250 r/min and 75 ml liquid medium in the size of 250 ml bottle. In this case, number of chlamydospores of ACCC30150 was up to 4.07×108/ml after incubation for 10 d. The best single-factor conditions for the production of chlamydospores of T. harziamum ACCC30371 were as follow: straw powder as medium, 28℃, pH6.0, 250 r/min and 75 ml liquid medium in the size of 250 ml bottle. The number of chlamydospores of ACCC30371 was up to 5.13×108/ml after incubation for 10 d.

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