花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效

花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效

王小兵^1,2,3, 骆永明^1,2, 刘五星¹, 李振高¹

1. 中国科学院土壤环境与污染修复重点实验室, 南京土壤研究所, 南京 210008;2. 中国科学院研究生院, 北京 100049;3. 扬州大学环境科学与工程学院, 扬州 225009

收稿日期:2010-04-01 修回日期:1900-01-01 出版日期:2011-01-18 发布日期:2011-01-18

Identification, Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt

WANG Xiao-bing^1,2,3, LUO Yong-ming^1,2, LIU Wu-xing¹, LI Zhen-gao¹

1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008;2. Graduate School of the Chinese Academy of Sciences, Beijing 100049;3. College of Environmental Sciences and Engineering, Yangzhou University, Yangzhou 225009, China

Received:2010-04-01 Revised:1900-01-01 Online:2011-01-18 Published:2011-01-18

摘要/Abstract

摘要： 从病区健康花生植株茎秆内分离到1株对花生青枯病菌Ralstonia solanacearum有强拮抗作用的内生细菌,命名为BZ6-1菌株。拮抗试验表明,该菌具有较广抗菌谱,对多种植物病原真菌有较强的抑制作用。形态观察和16S rDNA同源性分析初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。该菌的最佳培养基组成为: 糖蜜10g·L^-1,蛋白胨0.5g·L^-1,酵母膏0.5g·L^-1。最适发酵条件为pH8.5、装液量50mL/250mL、温度25℃、转速200r·min^-1、培养时间21h,在此条件下拮抗圈直径达34.0mm。田间试验结果表明BZ6-1菌株对花生青枯病防效达62.3%。

Abstract: An endophytic bacteria strain BZ6-1, isolated from the stem of healthy peanut plants in Ralstonia solanacearum-infested fields, suppressed R. solanacearum greatly and was of broad spectrum against plant pathogenic fungi. According to its morphology and 16S rDNA sequence, the strain BZ6-1 was preliminarily identified as Bacillus amyloliquefaciens. Diameter of inhibition zone reached 34.0 mm under the optimal fermentation conditions for antibiotic production, which was molasses 10 g, peptone 0.5 g and yeast extract 0.5 g in l L medium at pH 8.5, temperature 25 ℃, rotation rate 200 r·min^-1, medium amount 50 mL in 250 mL flask and incubation period 21 h. Field trials demonstrated that the control efficiency of strain BZ6-1 against peanut bacterial wilt disease was 62.3%.

中图分类号:

王小兵1,2,3 , 骆永明1,2 , 刘五星1 , 李振高1 . 花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效[J]. , DOI: .

WANG Xiao-bing^1,2,3, LUO Yong-ming^1,2, LIU Wu-xing¹, LI Zhen-gao¹. Identification, Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt[J]. , DOI: .

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