基于非靶向代谢组学的烟草镰刀菌根腐病和黑胫病拮抗链霉菌及其代谢产物的鉴定

doi:10.16409/j.cnki.2095-039x.2023.02.036

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (4): 875-884.DOI: 10.16409/j.cnki.2095-039x.2023.02.036

基于非靶向代谢组学的烟草镰刀菌根腐病和黑胫病拮抗链霉菌及其代谢产物的鉴定

刘鹤¹, 单宇航¹, 邱睿², 李淑君², 张崇¹, 吴元华¹, 安梦楠¹

1. 沈阳农业大学植物保护学院, 沈阳 110866;
2. 烟草行业黄淮烟区烟草病虫害绿色防控重点实验室/河南农业科学院烟草研究所, 许昌 461000

收稿日期:2022-11-08 出版日期:2023-08-25 发布日期:2023-08-25
通讯作者: 安梦楠, 副教授, E-mail:anmengnan@syau.edu.cn
作者简介:刘鹤，讲师，E-mail：2363598334@qq.com；单宇航，硕士研究生，E-mail：2019220469@stu.syau.edu.cn。
基金资助:
黄淮烟区烟草病虫害绿色防控重点实验室开放课题（2021KF01）；中国烟草总公司重大科技项目（110202101051（LS-11））

Identification of Streptomyces and Its Metabolites Antagonizing Fusarium oxysporum and Phytophthora parasitica in Tobacco Based on Non-Target Metabolomics

LIU He¹, SHAN Yuhang¹, QIU Rui², LI Shujun², ZHANG Chong¹, WU Yuanhua¹, AN Mengnan¹

1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;
2. Key Laboratory for Green Preservation & Control of Tobacco Diseases and Pests in Huanghuai Growing Area/Tobacco Research Institute, Henan Academy of Agricultural Sciences, Xuchang 461000, China

Received:2022-11-08 Online:2023-08-25 Published:2023-08-25

摘要/Abstract

摘要： 针对烟草两种土传病害病原菌-镰刀根腐病菌Fusarium oxysporum和黑胫病菌Phytophthora parasitica,本实验室前期从烟草根际土壤中筛选得到1株具有良好生防作用的链霉菌,结合形态学、生理生化特性及分子生物学分析,将其鉴定为淀粉酶产色链霉菌Streptomyces diastatochromogenes。通过对该生防菌发酵液进行色谱-质谱联用(LC/MS)非靶向代谢组学分析,确定其重要代谢产物为茴香霉素。利用高效液相色谱对产物进行验证及含量测定,表明每克粗提物中含有茴香霉素423.98 µg。菌丝生长抑制试验结果表明,茴香霉素对镰刀根腐病菌和黑胫病菌的EC₅₀值分别为1556.36和40.74 mg/mL。本研究为烟草镰刀菌根腐病和黑胫病的生物防治提供了新的方法,并为其代谢产物-茴香霉素的进一步利用提供了有价值的理论基础。

关键词: 烟草镰刀菌根腐病, 烟草黑胫病, 淀粉酶产色链霉菌, LC/MS非靶向代谢组学, 茴香霉素

Abstract: To develop effective biocontrol products against two soil-borne diseases, root rot (Fusarium oxysporum) and black shank (Phytophthora parasitica), a Streptomyces strain with good control effect was screened by our laboratory from tobacco rhizosphere soil. It was identified as Streptomyces diastatochromogenes by morphological, physiological, biochemical characteristics and molecular biological analysis. The main active metabolite of the biocontrol Streptomyces was identified as anisomycin by LC/MS non-target metabolomics analysis. The product was verified and content determined by high performance liquid chromatography, and it was found that anisamycin contained 423.98 µg per gram of crude extract. The inhibition results on mycelial morphology and mycelial growth showed that anisomycin could cause the mycelia of F. oxysporum and P. parasitica to expand and distort. The EC₅₀ values for F. oxysporum and P. parasitica were 1556.36 mg/mL and 40.74 mg/mL, respectively. This study provides a new source of biocontrol agents for tobacco root rot and black shank diseases, and provided valuable insights into the further use of anisomycin.

Key words: Fusarium oxysporum, Phytophthora parasitica, Streptomyces diastatochromogenes, LC/MS non-target metabolomics, anisomycin

中图分类号:

S476

刘鹤, 单宇航, 邱睿, 李淑君, 张崇, 吴元华, 安梦楠. 基于非靶向代谢组学的烟草镰刀菌根腐病和黑胫病拮抗链霉菌及其代谢产物的鉴定[J]. 中国生物防治学报, 2023, 39(4): 875-884.

LIU He, SHAN Yuhang, QIU Rui, LI Shujun, ZHANG Chong, WU Yuanhua, AN Mengnan. Identification of Streptomyces and Its Metabolites Antagonizing Fusarium oxysporum and Phytophthora parasitica in Tobacco Based on Non-Target Metabolomics[J]. Chinese Journal of Biological Control, 2023, 39(4): 875-884.

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基于非靶向代谢组学的烟草镰刀菌根腐病和黑胫病拮抗链霉菌及其代谢产物的鉴定

Identification of Streptomyces and Its Metabolites Antagonizing Fusarium oxysporum and Phytophthora parasitica in Tobacco Based on Non-Target Metabolomics

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