抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析

doi:10.16409/j.cnki.2095-039x.2024.02.020

中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (3): 630-640.DOI: 10.16409/j.cnki.2095-039x.2024.02.020

• 研究论文 • 上一篇

抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析

李玲玲¹, 陈奕冰¹, 滕凯², 肖志鹏², 周向平², 肖艳松², 蔡海林², 董鹏¹, 梅斯钇¹, 刘索妮¹, 伍绍龙², 刘天波², 唐前君¹

1. 湖南农业大学植物保护学院, 长沙 410128;
2. 中国烟草总公司湖南省公司, 长沙 410004

收稿日期:2023-09-20 发布日期:2024-06-07
通讯作者: 唐前君,教授,E-mail:Tangqianjun@126.com;刘天波,高级农艺师,E-mail:tianboliu@126.com。
作者简介:李玲玲,硕士研究生,E-mail:2643476710@qq.com

Screening, Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY

LI Lingling¹, CHEN Yibing¹, TENG Kai², XIAO Zhipeng², ZHOU Xiangping², XIAO Yansong², CAI Hailin², DONG Peng¹, MEI Siyi¹, LIU Suoni¹, WU Shaolong², LIU Tianbo², TANG Qianjun¹

1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Provincial Tobacco Company, Changsha 410004, China

Received:2023-09-20 Published:2024-06-07
Contact: 湖南省重点领域研发计划（2023NK2019);中国烟草总公司项目（110202101050（LS-10），110202201019（LS-03）);湖南省烟草公司衡阳市公司项目（HYYC2023KJ32）
Supported by:
10.16409/j.cnki.2095-039x.2024.02.020

摘要/Abstract

摘要： 由马铃薯Y病毒（Potato virus Y，PVY）引起的病毒病在烟草种植地区大面积发生，对烟草品质和产量产生重大影响。采用半叶枯斑法筛选出对烟草PVY防治效果优良的生防细菌D3-1，对PVY的枯斑抑制率达99.71%，经形态学、生理生化特性以及16S rDNA序列鉴定其为解淀粉芽胞杆菌Bacillus amyloliquefaciens。菌株D3-1具有广谱的抑菌能力，可抑制病原真菌和细菌生长，菌株D3-1发酵液（1×10⁹ CFU/mL）对烟草PVY的田间防治效果为63.67%。全基因组测序分析发现菌株D3-1基因组含有表面活性素surfactin、丰原素fengycin、嗜铁素bacillibactin等12种具有抑菌作用的次生代谢产物合成基因簇，经HPLC-MS定性分析确定了表面活性素surfactin和丰原素fengycin等32个脂肽类提取物。菌株D3-1对PVY有良好防治效果，具有作为防治烟草PVY生防菌剂的潜力。

关键词: PVY, Bacillus amyloliquefaciens, 全基因组测序, 次生代谢产物合成基因簇, 活性物质

Abstract: Viral diseases caused by potato virus Y (PVY) occur extensively in tobacco planting areas, significantly impacting tobacco quality and yield. Through half-leaf necrosis spot method, the biocontrol bacterium D3-1 with excellent efficacy against PVY in tobacco was screened, exhibiting an inhibition rate of 99.71%. Based on morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis, D3-1 was identified to be Bacillus amyloliquefaciens. D3-1 strain displayed broad-spectrum anti antimicrobial activity, inhibiting the growth of pathogenic fungi and bacteria. Field trials with D3-1 fermentation broth (1×10⁹ CFU/mL) resulted in an efficacy of 63.67% against PVY in tobacco. Whole-genome sequencing revealed that strain D3-1 harbored 12 gene clusters responsible for the synthesis of secondary metabolites, including surfactin, fengycin, and bacillibactin, known for their antimicrobial properties. Qualitative analysis using HPLC-MS confirmed the presence of 32 peptide extracts, including surfactin and fengycin. Strain D3-1 exhibited promising efficacy against PVY, demonstrating its potential as a biocontrol agent for managing PVY in tobacco.

Key words: PVY, Bacillus amyloliquefaciens, whole-genome sequencing, gene cluster of secondary metabolite synthesis, active substance

中图分类号:

S476

李玲玲, 陈奕冰, 滕凯, 肖志鹏, 周向平, 肖艳松, 蔡海林, 董鹏, 梅斯钇, 刘索妮, 伍绍龙, 刘天波, 唐前君. 抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析[J]. 中国生物防治学报, 2024, 40(3): 630-640.

LI Lingling, CHEN Yibing, TENG Kai, XIAO Zhipeng, ZHOU Xiangping, XIAO Yansong, CAI Hailin, DONG Peng, MEI Siyi, LIU Suoni, WU Shaolong, LIU Tianbo, TANG Qianjun. Screening, Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY[J]. Chinese Journal of Biological Control, 2024, 40(3): 630-640.

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抗烟草PVY生防细菌D3-1的筛选鉴定及活性物质分析

Screening, Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY

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