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中国生物防治学报 ›› 2024, Vol. 40 ›› Issue (5): 1009-1014.DOI: 10.16409/j.cnki.2095-039x.2022.03.025

• 研究论文 • 上一篇    

昆虫病原线虫对暗黑鳃金龟的致病力

张彩铃1,2, 王志敏2, 王思雨2, Ibrahim Osman2, 李克斌2, 张帅2, 曹雅忠2, 王森山1, 尹姣2   

  1. 1. 甘肃农业大学植物保护学院, 兰州 730070;
    2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193
  • 收稿日期:2024-02-26 发布日期:2024-10-11
  • 作者简介:张彩铃,硕士研究生,E-mail:m18854806920@163.com。通信作者,王森山,教授,E-mail:wangsenshan@gsau.edu.cn;尹姣,研究员,E-mail:jyin@ippcaas.cn。
  • 基金资助:
    国家重点研发计划(2023YFE0125800)

Virulence of Entomopathogenic Nematodes to Holotrichia parallela Larvae

ZHANG Cailing1,2, WANG Zhimin2, WANG Siyu2, Ibrahim Osman2, LI Kebin2, ZHANG Shuai2, CAO Yazhong2, WANG Senshan1, YIN Jiao2   

  1. 1. College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2024-02-26 Published:2024-10-11

摘要: 为了筛选防治暗黑鳃金龟Holotrichia parallela的优良昆虫病原线虫品系,本文通过室内生测,比较了7个昆虫病原线虫品系对暗黑鳃金龟1龄末幼虫的致病力,并明确了其最佳侵染条件。生测结果表明,不同昆虫病原线虫品系对暗黑鳃金龟1龄末幼虫均有一定的致病力,其中樱桃异小杆线虫Heterorhabditis bacteriophora(D26-6)对暗黑鳃金龟幼虫致病力最强,在处理9 d后,暗黑鳃金龟幼虫的校正死亡率达到了100%。此外,温度、土壤湿度以及线虫施用剂量对D26-6线虫的致病力均会产生一定的影响,其侵染暗黑鳃金龟幼虫的适宜温度为25℃、适宜土壤含水量为18%;当暗黑鳃金龟幼虫与D26-6线虫比为1:100时,处理11 d后暗黑鳃金龟幼虫的校正死亡率可达到100%。

关键词: 昆虫病原线虫, 樱桃异小杆线虫, 暗黑鳃金龟, 致病力

Abstract: The pathogenicity of seven entomopathogenic nematodes (EPNs) against the late 1st instar larvae of Holotrichia parallela was tested in laboratory to screen the effective strains of entomopathogenic nematodes for the H. parallela control, and the most suitable environmental conditions of strains were identified. Results showed that pathogenicity of seven EPNS strains were different under laboratory conditions, the Heterorhabditis bacteriophora (D26-6) had the strongest pathogenicity to the larvae of H. parallela. In addition, temperature, soil moisture and dosage of EPNs all take effects on the pathogenicity. The optimum temperature and soil moisture for H. bacteriophora (D26-6) infection were 25 ℃ and 18% respectively, when the ratio of H. parallela larvae to D26-6 nematodes was 1:100, the mortality of H. parallela larvae can reach 100% after 11 days.

Key words: entomopathogenic nematodes, Heterorhabditis bacteriophora, Holotrichia parallela, pathogenicity

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