简单近芽胞杆菌ZF535分离鉴定及其胞外多糖诱导黄瓜抗细菌性角斑病研究

doi:10.16409/j.cnki.2095-039x.2026.02.057

摘要/Abstract

摘要： 为筛选对黄瓜细菌性角斑病具有诱导抗病作用的生防菌株，分析其诱导抗病应用潜力，并为微生物菌剂研发提供优良菌种资源。本研究采用LB-苯胺蓝平板结合菌丝拉丝法定性筛选产胞外多糖（extracellular polysaccharides，EPS）细菌，通过EPS产量测定复筛高产菌株，并利用盆栽试验筛选对黄瓜细菌性角斑病防效显著的菌株。采用多基因序列分析鉴定菌种，利用平板对峙法检测抑菌活性。测定EPS灌根处理后的防效，分析黄瓜叶片防御酶活性、叶绿素、总酚、类黄酮及丙二醛（MDA）含量等诱抗指标。结果表明，获得一株EPS产量为1.67 g/L、盆栽防效为58.75%的菌株ZF535，经鉴定为简单近芽胞杆菌Peribacillus simplex，且该菌株无直接抑菌活性。盆栽试验显示，简单近芽胞杆菌ZF535胞外多糖处理对黄瓜细菌性角斑病的防效达60.43%，可显著提升黄瓜叶片防御酶活性，促进叶绿素、总酚及类黄酮含量增加，诱导胼胝质沉积，同时减少丙二醛（MDA）积累。表明菌株ZF535具有诱导抗病的潜力，是良好的生防资源，开发前景广阔。

关键词: 简单近芽胞杆菌, 细菌性角斑病, 胞外多糖, 诱导抗病

Abstract: This study aims to screen biocontrol strains that produce exopolysaccharides (EPS) and induce resistance against cucumber bacterial angular leaf spot, evaluate their potential for inducing disease resistance, and provide excellent strain resources for the development of microbial agents. LB-aniline blue plates combined with a string test were used to qualitatively screen EPS-producing bacteria. High-yielding EPS strains were further selected by measuring EPS production, and their efficacy against cucumber bacterial angular leaf spot was evaluated in pot experiments. The selected strain was identified by multi-gene sequence analysis, and its direct antagonistic activity was tested using the dual-culture method. After root drenching with EPS, the control efficacy was determined, and several defense-related indicators in cucumber leaves, including defense enzyme activities, chlorophyll, total phenolics, flavonoids, and malondialdehyde (MDA) content, were analyzed. The results showed that strain ZF535 was obtained, with an EPS yield of 1.67 g/L and a pot control efficacy of 58.75%. It was identified as Peribacillus simplex and exhibited no direct inhibitory antibacterial activity. pot experiments showed that EPS from P. simplex ZF535 achieved 60.43% control efficacy against cucumber bacterial angular leaf spot, significantly increased the activities of defense-related enzymes, raised the levels of chlorophyll, total phenolics and flavonoids, induced callose deposition, and reduced MDA accumulation in cucumber leaves. These results indicate that strain ZF535 has the potential to induce plant resistance and represents a promising biocontrol resource for future development.

Key words: Peribacillus simplex, angular leaf spot of cucumber, extracellular polysaccharides, induced resistance

中图分类号:

S6422

郭平平, 李俊辉, 王依, 谢学文, 石延霞, 谷医林, 王远宏, 李宝聚, 李磊. 简单近芽胞杆菌ZF535分离鉴定及其胞外多糖诱导黄瓜抗细菌性角斑病研究[J]. 中国生物防治学报, 2026, 42(5): 1202-1215.

GUO Pingping, LI Junhui, WANG Yi, XIE Xuewen, SHI Yanxia, GU Yilin, WANG Yuanhong, LI Baoju, LI Lei. Isolation and Identification of Peribacillus simplex ZF535 and Its Exopolysaccharide-Induced Resistance against Cucumber Bacterial Angular Leaf Spot[J]. Chinese Journal of Biological Control, 2026, 42(5): 1202-1215.

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