抗烟草花叶病毒1% fTDP蛋白可湿性粉剂的制备及生防评价

doi:10.16409/j.cnki.2095-039x.2026.02.048

摘要/Abstract

摘要： fTDP是上皮电阻降低蛋白（Transepithelial electrical resistance-decreasing protein,TDP）的大肠杆菌重组表达蛋白，能够诱导烟草产生对烟草花叶病毒（Tobacco mosaic virus,TMV）的抗性，为提升fTDP蛋白的田间应用效果，本研究以生物相容性与制剂质量指标为筛选依据，获得对TMV具有防治效果的1%fTDP可湿性粉剂配方，并进一步在盆栽及田间条件下验证其对烟草诱导抗性效果。结果表明，该制剂的最佳配方为吸附fTDP粗蛋白的硅藻土为载体（90%），添加十二烷基苯磺酸钠（5%）与亚甲基双甲基萘磺酸钠（5%）。温室盆栽结果显示，与fTDP蛋白溶液相比，1%fTDP可湿性粉剂的施用可显著降低烟草TMV含量，并可上调抗病相关基因NPR1、PR1、PR1a及PR1b的转录表达水平，提高超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）等防御酶活性。周期21 d的田间试验结果表明，1%fTDP蛋白可湿性粉剂对TMV的平均防效为49.6%，优于纯fTDP蛋白（39.2%），与8%宁南霉素的防效接近。本研究构建的fTDP蛋白可湿性粉剂配方，为蛋白诱抗剂剂型改良与田间抗病机制的后续研究提供了试验依据。

关键词: 诱导抗病性, 烟草花叶病毒, fTDP, 可湿性粉剂

Abstract: Transepithelial electrical resistance-decreasing protein(TDP), produced as a recombinant protein(fTDP) in Escherichia coli, can induce resistance to tobacco mosaic virus(TMV) in tobacco. To improve its performance under field conditions, formulation carriers and additives were screened based on biocompatibility and formulation quality parameters. A 1% fTDP wettable powder(WP) with activity against TMV was subsequently developed, and its resistance-inducing efficacy was evaluated in greenhouse pot experiments and field trials. The optimized formulation comprised diatomite loaded with crude fTDP as the carrier(90%), sodium dodecylbenzenesulfonate as the wetting agent(5%), and methylene bis-methylnaphthalene sulfonate as the dispersant(5%). In greenhouse experiments, treatment with the 1% fTDP WP significantly reduced TMV accumulation in tobacco plants compared with the fTDP protein solution. The formulation also upregulated the transcription of the defense-related genes NPR1, PR1, PR1a, and PR1b, and enhanced the activities of defense enzymes, including superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT). In a 21-day field trial, the 1% fTDP WP achieved an average control efficacy of 49.6% against TMV, which was superior to unformulated fTDP(39.2%) and comparable to 8% ningnanmycin. These findings provide an experimental basis for the future optimization of protein elicitor formulations formulations and further investigation of field disease-resistance mechanism.

Key words: inducing disease resistance, tobacco mosaic virus, fTDP, wettable powder

中图分类号:

S476

文旭, 刘鑫, 张焕然, 王建枫, 涂班武, 漆育婷, 吴丽萍. 抗烟草花叶病毒1% fTDP蛋白可湿性粉剂的制备及生防评价[J]. 中国生物防治学报, 2026, 42(4): 969-982.

WEN Xu, LIU Xin, ZHANG Huanran, WANG Jianfeng, TU Banwu, QI Yuting, WU Liping. Preparation and Biocontrol Efficacy of 1% fTDP Protein Wettable Powder on Tobacco Mosaic Virus[J]. Chinese Journal of Biological Control, 2026, 42(4): 969-982.

/ 推荐

参考文献

[1] Guo W, Yan H, Ren X, et al. Berberine induces resistance against tobacco mosaic virus in tobacco[J]. Pest Management Science, 2020, 76(5):1804-1813.
[2] Scholthof K B G, Adkins S, Czosnek H, et al. Top 10 plant viruses in molecular plant pathology[J]. Molecular Plant Pathology, 2011, 12(9):938-954.
[3] 邱德文,杨秀芬.蛋白质农药研究与产业化进展[J]. 中国农业科技导报, 2006, 8(6):1-4.
[4] 陈国参,王旭明,周付忠,等.蛋白质农药HrpNEcc防治大白菜软腐病试验[J]. 河南科学, 2011, 29(5):546-549.
[5] 赵梅勤,王磊,张兵,等.植物抗病激活蛋白harpinXooc防治水稻病害的研究[J]. 中国生物防治, 2006(4):283-289.
[6] 吴玉星,王亚娇,韩森,等. 6%寡糖·链蛋白可湿性粉剂与杀菌剂混用防治小麦白粉病的农药减施技术研究[J]. 植物保护, 2023, 49(3):292-297.
[7] 任杰,麻志欣,袁京京,等.植物免疫诱抗蛋白应用研究进展[J]. 现代农药, 2025, 24(6):1-6.
[8] 王以燕,袁善奎,农向群,等.我国微生物农药常见剂型种类及管理[J]. 中国生物防治学报, 2021, 37(4):640-645.
[9] 中捷四方生物科技股份有限公司,杨凌翔农业生物科技有限公司.一种含大丽轮枝孢激活蛋白的可湿性粉剂和应用[P]. 中国发明专利:202211145286.3.
[10] 李宏光,易图永,肖艳松,等. 7%井冈霉素·极细链格孢激活蛋白可湿性粉剂对烟草病毒病的防效研究[J]. 现代农业科技, 2014(24):126-131.
[11] Zhang Y, Gao C, Zhang Y, et al. FTX271:A potential gene resource for plant antiviral transgenic breeding[J]. Frontiers in Microbiology, 2022, 13:1003478.
[12] Han X, Zhang Y, Zhang Z, et al. Antiviral agent fTDP stimulates the SA signaling pathway and enhances tobacco defense against tobacco mosaic virus[J]. Pesticide Biochemistry and Physiology, 2022, 180:105002.
[13] 宋亚迪.马铃薯疮痂病生防链霉菌可湿性粉剂研制[D]. 呼和浩特:内蒙古农业大学, 2023.
[14] 张志云.原核表达产物TDP诱导烟草抗TMV的组学分析[D]. 南昌:南昌大学, 2021.
[15] 方宏筠,李大力,陈秀玲,等.植物系统获得抗性的抗病基因及其应用[M] //植物基因工程.第2版.北京:科学出版社, 2024, 64.
[16] Mao H, Yang X F, Zeng H M, et al. Purification and expression of a protein elicitor from Alternaria tenuissima and elicitor-mediated defence responses in tobacco[J]. Physiological and Molecular Plant Pathology, 2010, 74(2):128-134.
[17] Liu Y, Zhou X P, Liu W B, et al. HpaXpm, a novel harpin of Xanthomonas phaseoli pv. manihotis, acts as an elicitor with high thermal stability, reduces disease, and promotes plant growth[J]. BMC Microbiology, 2020, 20(1):318.
[18] Li C, Wang Y, Zhang J, et al. Thermostable elicitor from Colletotrichum fructicola interacts with PbrPOD1 to promote pear resistance and protect against bitter rot disease[J]. Journal of Agricultural and Food Chemistry, 2015, 63(42):9218-9226.
[19] 张维,吕朝阳,李博雅,等.甲基营养型芽孢杆菌NKG-1可湿性粉剂的制备及药效研究[J]. 中国农学通报, 2022, 38(15):130-138.
[20] 郭庄园,杨成德,金梦军,等.枯草芽胞杆菌262XY2′可湿性粉剂的研制[J]. 中国生物防治学报, 2022, 38(2):414-420.
[21] 申云鑫,李铭刚,施竹凤,等.贝莱斯芽胞杆菌SH-1471可湿性粉剂研制及其对番茄枯萎病的防治效果[J]. 中国生物防治学报, 2023, 39(4):904-914.
[22] 李姝江,方馨玫,曾艳玲,等.解淀粉芽孢杆菌BA-12可湿性粉剂研制及对核桃根腐病的防治效果[J]. 中国生物防治学报, 2016, 32(5):619-626.
[23] Li W J, Yu R, Liu W J, et al. RNA silencing is activated by N gene-mediated hypersensitive response and plays a key role in local and systemic virus resistance[J]. Phytopathology Research, 2024, 6(52):1-13.
[24] Takagi K, Tasaki K, Komori H, et al. Hypersensitivity-related genes hsr201 and hsr203j are regulated by calmodulin-binding protein 60-type transcription factors and required for pathogen signal-induced salicylic acid synthesis[J]. Plant and Cell Physiology, 2022, 63(7):1008-1022.
[25] Liu T, Chen T, Kan J, et al. The Gh MYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants[J]. Plant Biotechnology Journal, 2022, 20(4):722-735.
[26] Zavaliev R, Mohan R, Chen T, et al. Formation of NPR1 condensates promotes cell survival during the plant immune response[J]. Cell, 2020, 182(5):1093-1108.e18.
[27] Backer R, Skorokhodova O, Zavaliev R. NPR1, a key immune regulator for plant survival under biotic and abiotic stresses[J]. Plant Physiology, 2023,181(4):1823-1842.
[28] Wang J X, Liu S, Ren P, et al. A novel protein elicitor(PeSy1)from Saccharothrix yanglingensis induces plant resistance and interacts with a receptor-like cytoplasmic kinase in Nicotiana benthamiana[J]. Molecular Plant Pathology, 2023, 24(5):436-451.
[29] Wang J F, Zhang H R, Wen X, et al. Chitosan nanocarriers enhance stability and field performance of fTDP against plant viruses[J]. Pesticide Biochemistry and Physiology, 2025, 213:106547.