Abstract: Vegetative insecticidal protein Vip3 is a class of novel insecticidal proteins which secreted by many Bacillus thuringiensis strains during their vegetative growth phase. These proteins have high toxicity against a number of lepidopteran pests. They share low homology with δ-endotoxin of B. thuringiensis but share a high level of sequence similarity with others in this family. In this paper, we reviewed the advances on Vip3 research, including gene discovery, features, receptors, mode of action and applications, etc.