淡紫紫孢菌防治采后番茄果实灰霉病

doi:10.16409/j.cnki.2095-039x.2017.04.013

中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (4): 525-530.DOI: 10.16409/j.cnki.2095-039x.2017.04.013

淡紫紫孢菌防治采后番茄果实灰霉病

袁和奇, 徐鹏程, 肖海艳, 张臻, 赵娟, 王高峰, 肖雪琼, 肖炎农

华中农业大学植物科学技术学院/湖北省植物病害监测与安全控制重点实验室, 武汉 430070

收稿日期:2017-02-06 出版日期:2017-08-08 发布日期:2017-08-11
通讯作者: 肖炎农,博士,教授,博士生导师,E-mail:xiaoyannong@mail.hzau.edu.cn
作者简介:袁和奇,硕士研究生,E-mail:yuanheqi15@163.com
基金资助:
国家公益性行业（农业）科研专项（201303025）

Efficacy of Purpureocillium lilacinus on Gray Mold of Post-harvest Tomato Fruits

YUAN Heqi, XU Pengcheng, XIAO Haiyan, ZHANG Zhen, ZHAO Juan, WANG Gaofeng, XIAO Xueqiong, XIAO Yannong

The Key Laboratory of Plant Pathology of Hubei Province/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2017-02-06 Online:2017-08-08 Published:2017-08-11

摘要/Abstract

摘要： 本文通过孢子萌发检测、平板生长抑制法和果实病害防治试验，测定了淡紫紫孢菌Purpureocillium lilacinus PL36-1防治储藏期番茄果实灰霉病的效果。结果表明，菌株PL36-1发酵滤液不仅能够抑制灰葡萄孢分生孢子萌发和芽管伸长，而且可以抑制灰葡萄孢菌丝生长。对峙培养的菌株PL36-1显著抑制灰葡萄孢的菌丝生长，菌丝生长抑制率可达到74.45%，菌株PL36-1产生的挥发性物质也对灰葡萄孢菌丝生长有一定的抑制作用。菌株PL36-1发酵液处理樱桃番茄果实后，能有效控制番茄果实灰霉病的发生，含有2×10⁸孢子/mL的发酵液10 d的防治效果达70.64%。

关键词: 淡紫紫孢菌, 番茄灰霉病, 采后病害, 抑菌作用

Abstract: The effect of Purpureocillium lilacinus PL36-1 on gray mold of post-harvest tomato fruits was evaluated. The results showed that the fermentation filtrate of strain PL36-1 inhibited mycelial growth of Botrytis cinerea, as well as conidial germination and elongation of germ tubes. Mycelial growth of B. cinerea decreased by 74.45% when it was dual cultured with the strain PL36-1 in petri dish. The VOCs from PL36-1 also suppressed the mycelial growth of B. cinerea. The PL36-1 could control post-harvest tomato fruits gray mold effectively, the incidence of the disease fell by 70.64% after 10 days treated by PL36-1 fermentation broth with 2×10⁸ spores/mL. It was suggested that P. lilacinus was potential to be used as a biocontrol agent in management of post-harvest tomato fruits gray mold.

Key words: Purpureocillium lilacinus, tomato gray mold, post-harvest disease, inhibiting effect

中图分类号:

S476

袁和奇, 徐鹏程, 肖海艳, 张臻, 赵娟, 王高峰, 肖雪琼, 肖炎农. 淡紫紫孢菌防治采后番茄果实灰霉病[J]. 中国生物防治学报, 2017, 33(4): 525-530.

YUAN Heqi, XU Pengcheng, XIAO Haiyan, ZHANG Zhen, ZHAO Juan, WANG Gaofeng, XIAO Xueqiong, XIAO Yannong. Efficacy of Purpureocillium lilacinus on Gray Mold of Post-harvest Tomato Fruits[J]. journal1, 2017, 33(4): 525-530.

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淡紫紫孢菌防治采后番茄果实灰霉病

Efficacy of Purpureocillium lilacinus on Gray Mold of Post-harvest Tomato Fruits

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