粘虫过氧化物酶MsPOD基因的克隆及不同温度胁迫的诱导效应

doi:10.16409/j.cnki.2095-039x.2019.01.011

摘要/Abstract

摘要： 过氧化物酶（POD）是昆虫体内一种很关键的抗氧化酶，在维持昆虫体内氧化还原的动态平衡及保护昆虫免受氧化损伤方面起到重要的作用。本试验通过转录组测序方法获得一条粘虫过氧化物酶基因的cDNA序列，该序列命名为MsPOD（GenBank登录号：MH606240）。该序列全长2433 bp，开放阅读框长度为2061 bp，编码686个氨基酸组成的多肽，分子量约76.1 ku，等电点5.68，具有1个标志性的动物亚铁血红素过氧化物酶细胞粘附蛋白结构域。氨基酸序列比对表明，MsPOD的氨基酸序列与鳞翅目夜蛾科其他昆虫亲缘关系较近，其中与棉铃虫POD氨基酸序列同源性最高，达92%。基因表达水平研究发现，MsPOD基因在不同发育阶段和不同组织中差异表达，在蛹期和唾腺中表达量最高。温度梯度诱导后，MsPOD基因在不同时间点的表达量具有显著差异，经35℃下12 h处理后表达量最高。研究结果为进一步研究过氧化物酶在昆虫体内的保护性作用以及利用该基因进行分子设计来防治粘虫奠定理论基础。

关键词: 粘虫, 过氧化物酶, 克隆, 温度, 诱导

Abstract: Peroxidase is a key antioxidant enzyme in insects and plays an important role in maintaining redox homeostasis in insects and protecting insects from oxidative damage. In this study, a novel peroxidase cDNA sequence MsPOD (GenBank accession:No. MH606240) was obtained from Mythimna separata by transcriptome sequencing technology. The full-length cDNA sequence MsPOD was 2433 bp, with ORF length of 2061 bp, encoding a polypeptide consisting of 686 amino acids. The peptide was characterized by a molecular mass of about 76.1 ku and an isoelectric point of about 5.68, and distinct domain of animal heme peroxidase cell adhesion protein. Amino acids alignment analysis showed that MsPOD amino acid sequence was close in homology to that of other Noctuidae insects, with the highest homology of 92% with that in Helicoverpa armigera. MsPOD expressed specifically at different developmental stages and in different tissues, the highest expression occurring at pupal stage and in salivary glands. Expression of MsPOD also varied with different temperatures, reaching the highest level at 12 h post treatment at 35℃. These results lay a foundation for the further study of protective effects of MsPOD and utilizing it to design molecular method to control M. separata.

Key words: Mythimna separata, peroxidase, cloning, temperature, induction

中图分类号:

S476

杨航, 王晓云, 裴海英, 樊东. 粘虫过氧化物酶MsPOD基因的克隆及不同温度胁迫的诱导效应[J]. 中国生物防治学报, 2019, 35(1): 44-52.

YANG Hang, WANG Xiaoyun, PEI Haiying, FAN Dong. Cloning a Peroxidase cDNA Sequence from the Oriental Armyworm, Mythimna separata Walker and Its Induction to Different Temperature Stress[J]. journal1, 2019, 35(1): 44-52.

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