麦长管蚜电压门控钠离子通道基因克隆及序列分析

doi:10.16409/j.cnki.2095-039x.2020.02.018

中国生物防治学报 ›› 2020, Vol. 36 ›› Issue (2): 307-314.DOI: 10.16409/j.cnki.2095-039x.2020.02.018

麦长管蚜电压门控钠离子通道基因克隆及序列分析

段文波^1,2, 王颢^1,2, 李芬^2,3, 尹新明¹, 吴少英^1,2,3

1. 河南农业大学植物保护学院, 郑州 450002;
2. 海南大学植物保护学院, 海口 570228;
3. 海南大学热带农林生物灾害绿色防控教育部重点实验室, 海口 570228

收稿日期:2019-07-15 出版日期:2020-04-08 发布日期:2020-04-16
通讯作者: 尹新明,教授,E-mail:xinmingyin@hotmail.com;吴少英,教授,E-mail:wsywsy6000@hainanu.edu.cn
作者简介:段文波,硕士研究生,E-mail:1339032169@qq.com;王颢,硕士研究生,E-mail:2387226387@qq.com
基金资助:
海南大学启动基金（KYQD-ZR-1963）；中国博士后基金（2018M640675）

Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)

DUAN Wenbo^1,2, WANG Hao^1,2, LI Fen^2,3, YIN Xinming¹, WU Shaoying^1,2,3

1. College of Plant Protection, Henan Agricultural University, Henan Province, Zhengzhou 450002, China;
2. College of Plant Protection, Hainan University, Haikou 570228, China;
3. Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Hainan University, Ministry of Education, Haikou 570228, China

Received:2019-07-15 Online:2020-04-08 Published:2020-04-16

摘要/Abstract

摘要： 克隆获得麦长管蚜电压门控钠离子通道基因cDNA序列，明确其典型特征，为研究麦长管蚜抗性分子机理奠定基础。采用RT-PCR技术，克隆麦长管蚜钠离子通道基因cDNA序列，利用DNASTAR Lasergene 7.10软件对其序列进行分析。克隆得到两条cDNA序列SaNa_v1和SaNa_v2（GenBank登录号分别为MN176137和MN161584），SaNa_v1序列长3423 bp，共编码1141个氨基酸；SaNa_v2序列长2874 bp，共编码958个氨基酸。同源比对发现，SaNav1与禾谷缢管蚜和桃蚜的第一部分钠离子通道基因相似度分别高达97.81%和97.91%；SaNav2与禾谷缢管蚜和桃蚜的第二部分钠离子通道基因相似性高达97.90%和96.43%。所克隆序列包含4个同源结构域，每个结构域6个跨膜片段（S1～S6），存在钠通道选择性关键残基“DENS”。本文成功克隆了麦长管蚜中钠离子通道基因，为麦长管蚜钠离子通道抗性机制的研究提供依据。

关键词: 麦长管蚜, 钠离子通道, 基因克隆, 击倒抗性

Abstract: The cDNA sequences of voltage-gated sodium channel gene of Sitobion avenae were cloned and its typical characteristics were identified, which laid a foundation for further studies of the molecular mechanism of pesticide resistance in S. avenae. The sequences were cloned by RT-PCR and analyzed by DNASTAR Lasergene 7.10 software. Two cDNA sequences were obtained, SaNav1 and SaNav2, with GenBank accession numbers of MN176137 and MN161584, respecitvely. The SaNav1 sequence was 3423 bp, encoding a total of 1141 amino acids. The SaNav2 sequence was 2874 bp, encoding a total of 958 amino acids. The homology alignment found that the similarity of SaNav1 and the part I sodium channel genes of Rhodolospi maidis (XP_026806613) and Myzus persicae (XP_022174241) were as high as 97.81% and 97.91%; the similarity of SaNav2 and the part II sodium channel genes of Rhodolospi maidis (ALB75309.1) and Myzus persicae (XP_022174237.1) were as high as 97.90% and 96.43%, respectively. The cloned sequences contain four homologous domains, each with six transmembrane segments (S1—S6) and a sodium channel-selective key residue "DENS". The sodium channel genes cloned in S. avenae will provide a basis for studying the role of sodium channel in resistance mechanism in S. avenae.

Key words: Sitobion avenae, sodium channel, gene cloning, knockdown resistance

中图分类号:

S476

段文波, 王颢, 李芬, 尹新明, 吴少英. 麦长管蚜电压门控钠离子通道基因克隆及序列分析[J]. 中国生物防治学报, 2020, 36(2): 307-314.

DUAN Wenbo, WANG Hao, LI Fen, YIN Xinming, WU Shaoying. Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)[J]. Chinese Journal Of Biological Control, 2020, 36(2): 307-314.

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麦长管蚜电压门控钠离子通道基因克隆及序列分析

Cloning and Sequences Analysis of Voltage-Gated Sodium Channel Gene in Sitobion avenae (Fabricius)

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