沼泽红假单胞菌蛋白Rhp-PSP对烟草花叶病毒的系统抗性诱导及互作蛋白筛选

doi:10.16409/j.cnki.2095-039x.2021.06.00

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (5): 1073-1081.DOI: 10.16409/j.cnki.2095-039x.2021.06.00

沼泽红假单胞菌蛋白Rhp-PSP对烟草花叶病毒的系统抗性诱导及互作蛋白筛选

周寒梅¹, 陈丽洁¹, 翟忠英¹, 张德咏^1,2, 苏品^1,2, 刘勇^1,2

1. 湖南大学研究生院隆平分院, 长沙 410125;
2. 湖南省农业科学院植物保护研究所, 长沙 410125

收稿日期:2020-11-25 出版日期:2021-10-08 发布日期:2021-10-28
通讯作者: 刘勇,博士,研究员,E-mail:haoasliu@163.com。
作者简介:周寒梅,研究生,zhm9400@hnu.edu.cn
基金资助:
国家重点研发计划（2017YFD0200400）；新疆农业科学院科技创新重点培育专项（xjkcpy-2020005）；湖南省重点研发计划（2019NK2192）

Systemic Resistance to Tobacco Mosaic Virus (TMV) Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein

ZHOU Hanmei¹, CHEN Lijie¹, ZHAI Zhongying¹, ZHANG Deyong^1,2, SU Pin^1,2, LIU Yong^1,2

1. Longping Branch, Graduate School of Hunan University, Changsha 410125, China;
2. Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China

Received:2020-11-25 Online:2021-10-08 Published:2021-10-28

摘要/Abstract

摘要： 本文研究了蛋白Rhp-PSP诱导烟草抗TMV过程中相关防御酶的活性以及丙二醛（MDA）含量的变化，同时测定了抗病相关基因的表达情况。试验证明，蛋白Rhp-PSP处理烟草后，叶片中苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）的活性显著提高，但降低了MDA含量。同时基因PR-1、PR-3、PR-5、PDF1.2表达显著上调，证明了蛋白Rhp-PSP具有诱导烟草产生抗TMV的活性。为阐明诱导抗性机制，利用酵母双杂交系统从烟草cDNA文库进行互作蛋白的筛选并验证，结果表明GPI锚定蛋白、VAN3结合蛋白及叶绿素结合蛋白A与蛋白Rhp-PSP互作。

关键词: 关键字:沼泽红假单胞菌, 防御酶, 抗病相关基因, 蛋白互作

Abstract: The activity of defense enzymes, content of malondialdehyde (MDA) and expression level of resistance gene were determined in TMV inoculated tobacco plants induced by protein Rhp-PSP systemic resistance to TMV. The results showed that protein Rhp-PSP treatment increased the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in TMV inoculated tobacco leaves, but decreased the content of MDA, and up-regulated the gene expression of PR-1, PR-3, PR-5 and PDF1.2 significantly. The above results indicated that protein Rhp-PSP of Rhodopseudomonas palustris could induce systemic resistance to TMV in tobacco. In order to understand the mechanism of induce systemic resistance, the interaction proteins with protein Rhp-PSP were screened in the tobacco cDNA library using yeast two-hybrid system. The result showed that the GPI anchoring protein, VAN3 binding protein, chlorophyll binding protein A may have interaction with Rhp-PSP.

Key words: Rhodopseudomonas palustris, defense-related enzyme, plant defense-related genes, protein interaction

中图分类号:

S476

周寒梅, 陈丽洁, 翟忠英, 张德咏, 苏品, 刘勇. 沼泽红假单胞菌蛋白Rhp-PSP对烟草花叶病毒的系统抗性诱导及互作蛋白筛选[J]. 中国生物防治学报, 2021, 37(5): 1073-1081.

ZHOU Hanmei, CHEN Lijie, ZHAI Zhongying, ZHANG Deyong, SU Pin, LIU Yong. Systemic Resistance to Tobacco Mosaic Virus (TMV) Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein[J]. Chinese Journal of Biological Control, 2021, 37(5): 1073-1081.

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沼泽红假单胞菌蛋白Rhp-PSP对烟草花叶病毒的系统抗性诱导及互作蛋白筛选

Systemic Resistance to Tobacco Mosaic Virus (TMV) Induced by Protein Rhp-PSP of Rhodopseudomonas palustris and Screening of Interaction Protein

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