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中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (3): 573-579.DOI: 10.16409/j.cnki.2095-039x.2022.05.001

• 研究论文 • 上一篇    

球孢白僵菌与苦参碱混用对双线卷裙夜蛾的协同增效作用

关朝阳, 周国英, 李聪, 刘君昂   

  1. 中南林业科技大学/南方人工林病虫害防控国家林业局重点实验室/森林有害生物防控湖南省重点实验室/经济林培育与保护教育部重点实验室, 长沙 410004
  • 收稿日期:2021-03-16 发布日期:2022-06-20
  • 通讯作者: 刘君昂,博士,教授,E-mail:522375325@qq.com。
  • 作者简介:关朝阳,硕士,E-mail:m1506354498@163.com
  • 基金资助:
    国家重点研发计划(2018YFD0600202)

Synergistic Effect of Mixed Use of Beauveria bassiana and Matrine on Spodoptera bilinealis

GUAN Chaoyang, ZHOU Guoying, LI Cong, LIU Junang   

  1. Central South University of Forestry and Technology/Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South China/Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests/Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Changsha 410004, China
  • Received:2021-03-16 Published:2022-06-20

摘要: 本文研究了联合应用球孢白僵菌HB-P-04与低剂量苦参碱对双线卷裙夜蛾的杀虫效果,探讨球孢白僵菌与杀虫剂混用的不同使用顺序和时间间隔是否具有协同增效作用。结果表明,低剂量的苦参碱对菌株HB-P-04的孢子萌发、菌丝生长和产孢量的影响较小,相容性良好。当球孢白僵菌与苦参碱体积比为1:4时的共毒系数最高,达到223.48,具有最高的联合毒力效果,有显著的增效作用。先球孢白僵菌处理,间隔24 h后苦参碱处理的混用方案表现出显著的协同增效作用,9 d的校正死亡率达到92.86%。在室内毒力试验中,不论是球孢白僵菌与苦参碱以体积比1:4混用,还是先球孢白僵菌处理,间隔24 h后苦参碱处理的混用方案,均能有效地缩短杀虫时间,提高杀虫效率。

关键词: 球孢白僵菌, 双线卷裙夜蛾, 杀虫剂, 协同作用, 无公害防治

Abstract: This paper measured the insecticidal activity of Beauveria bassiana HB-P-04 combined with low-dose matrine against Spodoptera bilinealis, and the synergistic effect between B. bassiana and matrine in different use orders and time intervals. The results showed that low dose matrine had little effect on spore germination, mycelial growth and sporulation of strain HB-P-04, indicating that matrine is compatible with HB-P-04. When B. bassiana was mixed with matrine at a volume ratio of 1:4, the co-toxicity coefficient was the highest, reaching 223.48, showing significant synergistic effect. When B. bassiana was applied first and matrine was applied at 24 h interval, the treatment showed obvious synergistic effect, with a corrected mortality rate of 92.86% at 9 d post treatment. In the laboratory toxicity test, both the mixture of B. bassiana and matrine at a volume ratio of 1:4 and the treatment of B. bassiana firt and then matrine at 24 h interval effectively shortened the insecticidal time and improved the insecticidal efficiency.

Key words: Beauveria bassiana, Spodoptera bilinealis, insecticide, synergy, pollution free prevention

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