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中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (6): 1103-1110.DOI: 10.16409/j.cnki.2095-039x.2021.00.010

• 草地贪夜蛾生物防治专栏 • 上一篇    

苏云金杆菌G033A对化学农药防治草地贪夜蛾的减量效应

胡飞1, 徐婷婷1, 胡本进1, 毕思佳1, 佟强1, 徐丽娜1, 张杰2   

  1. 1. 安徽省农业科学院植物保护与农产品质量安全研究所, 合肥 230031;
    2. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室, 北京 100193
  • 收稿日期:2021-10-21 发布日期:2021-12-15
  • 通讯作者: 徐丽娜,副研究员,E-mail:caasxln@163.com;张杰,研究员,E-mail:jzhang@ippcaas.cn。
  • 作者简介:胡飞,副研究员,E-mail:hufly0224@163.com。
  • 基金资助:
    国家重点研发计划(2019YFD0300105);安徽省重大科技项目(201903a6020027);安徽省农业科学院科研团队(2021YL046)

Control Efficacy of Biopesticide Bacillus thuringiensis G033A Combined with Reduced Low Dose Chemical Pesticides on Spodoptera frugiperda

HU Fei1, XU Tingting1, HU Benjin1, BI Sijia1, TONG Qiang1, XU Lina1, ZHANG Jie2   

  1. 1. Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2021-10-21 Published:2021-12-15

摘要: 为提高生物农药田间使用效率,减少化学农药使用量,将苏云金杆菌G033A制剂与化学杀虫剂减量联合应用,进行草地贪夜蛾室内生测和田间药效试验。室内试验结果显示,5%甲维盐(0.2 g/L、0.15 g/L、0.1 g/L、0.05 g/L)、200 g/L氯虫苯甲酰胺(0.3 g/L、0.225 g/L、0.15 g/L、0.075 g/L)、15%甲维盐·氯虫苯甲酰胺(0.3 g/L、0.225 g/L、0.15 g/L、0.075 g/L)处理24 h后,草地贪夜蛾3龄幼虫的校正死亡率分别为99.07%、87.04%、85.19%、80.56%; 20.37%、11.11%、6.48%、1.85%; 68.52%、66.67%、60.19%、27.78%。而上述3种化学药剂各剂量与1.5 g/L G033A联合使用后,幼虫校正死亡率分别为100.00%、99.07%、91.67%、87.96%; 58.33%、25.93%、23.15%、8.33%; 83.33%、77.78%、63.89%、41.67%;均显著高于化学药剂单独使用。田间试验结果显示,5%甲维盐在参考剂量基础上减量25%、50%和G033A联合施用15 d后,对草地贪夜蛾的防效分别为66.77%和70.64%,显著高于其推荐剂量单独施用的防效44.43%。200 g/L氯虫苯甲酰胺及15%甲维盐·氯虫苯甲酰胺在参考剂量基础上分别减量25%、50%和G033A联合使用5 d和15 d后,对草地贪夜蛾的防效均高于其参考剂量单独施用。结果表明,G033A与化学药剂减量联合使用不仅可以显著提高G033A的杀虫活性,而且可以提高化学药剂的持效性,并改善速效性。田间亩施用G033A(75 g)+5%甲维盐(5 g)、G033A(75 g)+200 g/L氯虫苯甲酰胺(11.25 g)、G033A(75 g)+15%甲维盐·氯虫苯甲酰胺(15 g)、G033A(75 g)+15%甲维盐·氯虫苯甲酰胺(10 g)均对草地贪夜蛾具有较好的防治效果。

关键词: 苏云金杆菌G033A, 化学杀虫剂, 减量使用, 草地贪夜蛾

Abstract: To improve the application efficiency of biological pesticides in the field and reduce the use of chemical pesticides, the laboratory bioassay and field trials for control of Spodoptera frugiperda were conducted through combination of Bacillus thuringiensis G033A and reduced dosages of chemical pesticides. It was showed that the corrected mortality rates of the 3rd instar larvae were 99.07%, 87.04%, 85.19%, 80.56%; 20.37%, 11.11%, 6.48%, 1.85%; and 68.52%, 66.67%, 60.19%, 27.78%, respectively, after 24 h treated through 5% emamectin benzoate (0.2 g/L, 0.15 g/L, 0.1 g/L, 0.05 g/L), 200 g/L chlorantraniliprole (0.3 g/L, 0.225 g/L, 0.15 g/L, 0.075 g/L), 15% emamectin·chlorantraniliprole (0.3 g/L, 0.225 g/L, 0.15 g/L, 0.075 g/L). However, the mortality rates of larvae were 100.00%, 99.07%, 91.67% and 87.96%; 58.33%, 25.93%, 23.15% and 8.33%; 83.33%, 77.78%, 63.89% and 41.67%, when the 3 pesticides above were combined used with G033A (1.5 g/L), which were significantly higher than the pesticides used alone. Field experiments showed that after 15 days of combined application of reduced 25%, 50% dosage of emamectin benzoate and G033A, the control effects were 66.77% and 70.64%, respectively, which were significantly higher than the control effects of 44.43% of the reference dose used alone. The control effect of chlorantraniliprole, or emamectin·chlorantraniliprole combined with G033A for 5 and 15 days were higher than the reference dose of two chemical pesticides used alone. Field experiments showed that the combined use of G033A and reduced dosage of chemical pesticides could not only significantly improve the insecticidal activity of G033A, but also improve the efficacy of chemical pesticides and its quick efficacy. G033A (75 g) +5% emamectin benzoate (5 g), G033A (75 g) +200 g/L chlorantraniliprole (11.25 g), G033A (75 g) +15% emamectin·chlorantraniliprole (15 g), G033A (75 g) +15% emamectin·chlorantraniliprole (10 g) all have good control effect on Spodoptera frugiperda in field.

Key words: Bacillus thuringiensis G033A, chemical pesticides, reduced dose, Spodoptera frugiperda

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