对褐飞虱有功能效应的水稻挥发物活性组分的筛选与鉴定

doi:10.16409/j.cnki.2095-039x.2023.01.032

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (6): 1474-1481.DOI: 10.16409/j.cnki.2095-039x.2023.01.032

对褐飞虱有功能效应的水稻挥发物活性组分的筛选与鉴定

王兴云^1,2,3, 张新强¹, 李菁¹, 黄小玲⁴, 张元臣^1,3, 张坤朋¹, 王景顺^1,3

1. 安阳工学院生物与食品工程学院, 安阳 455000;
2. 安阳工学院博士后创新实践基地, 安阳 455000;
3. 河南省太行山林业有害生物野外科学观测研究站, 安阳 455000;
4. 广西省桂林市农业农村科教站, 桂林 541000

收稿日期:2022-09-05 出版日期:2023-12-08 发布日期:2023-12-20
通讯作者: 王兴云
作者简介:王兴云,博士,副教授,E-mail:wangxingyun402@163.com。
基金资助:
国家自然科学基金（31802007）；河南省高等学校重点科研项目（19A210007）；中国博士后基金（2020M672204）；河南省博士后科研项目启动项目（201902042）；安阳工学院博士后科研启动基金（BHJ2019002）

Screening and Identification of Active Components of Rice Volatiles with Functional Effects on Brown Planthopper, Nilaparvata lugens (Stål)

WANG Xingyun^1,2,3, ZHANG Xinqiang¹, LI Jing¹, HUANG Xiaoling⁴, ZHANG Yuanchen^1,3, ZHANG Kunpeng¹, WANG Jingshun^1,3

1. College of Biological and Food Engineering, Anyang Institute of Technology, Anyang 455000, China;
2. Postdoctoral Innovation Practice Base, Anyang Institute of Technology, Anyang 455000, China;
3. Field Scientific Observation and Research Station of Forest Pests in Taihang Mountain of Henan Province, Anyang 455000, China;
4. Guilin Agricultural and Rural Science and Education Station, Guilin 541000, China

Received:2022-09-05 Online:2023-12-08 Published:2023-12-20

摘要/Abstract

摘要： 为明确抗褐飞虱水稻品种Mudgo和感褐飞虱水稻品种TN1释放的挥发物中化学组分的差异，筛选鉴定对褐飞虱具有功能效应的活性化合物。本研究通过顶空动态收集装置获取Mudgo和TN1水稻的挥发物，采用气相色谱-质谱联用仪（GC-MS）筛选、鉴定水稻挥发物的活性组分，基于筛选鉴定出的Mudgo与TN1水稻挥发物中的差异活性化合物，采用Y型嗅觉仪对其进行功能验证。本研究在两水稻品种的挥发物中共筛选鉴定到52种化合物，其中Mudgo和TN1分别鉴定到27种，仅在Mudgo挥发物中存在的化合物有10种，仅在TN1挥发物中存在的化合物有15种。基于Mudgo和TN1水稻挥发物的差异活性组分鉴定结果，筛选出11种活性化合物进行功能验证。Y型嗅觉仪测定结果表明：低浓度（2 mL/mL）的芳樟醇、高浓度（200 mL/mL）的β-倍半水芹烯和水杨酸甲酯这3种化合物对褐飞虱具有显著的驱避作用，可发展用于褐飞虱防治的驱避剂；D-柠檬烯、β-石竹烯和高浓度的2-庚酮、2-乙基苯胺和姥鲛烷这5种化合物对褐飞虱具有显著的引诱作用，可发展用于褐飞虱防治的引诱剂。该研究可为褐飞虱的绿色防控和害虫综合治理提供科学数据和理论依据。

关键词: 褐飞虱, 水稻挥发物, 功能效应, 筛选与鉴定, 选择响应

Abstract: The difference of chemical components of rice volatiles released by the resistant variety Mudgo and the susceptible variety TN1 was assessed in relation to their functional effects on the brown planthopper, Nilaparvata lugens (Stål). Volatiles were obtained by headspace dynamic collection, and the active components of rice volatiles were screened and identified by gas chromatography-mass spectrometry (GC-MS). The functions of the differentially active volatiles of Mudgo and TN1 rice were verified by Y-type. As a result, a total of 52 volatile chemicals were identified in the volatiles of the two rice varieties, among which 27 chemicals were identified in both Mudgo and TN1, 10 chemicals were present only in Mudgo and 15 chemicals were present only in TN1. Further, 11 volatile chemicals were screened for functional verification. The Y-type results showed that linalool at low concentration (2 mL/mL), β-sesquahydralene at high concentration (200 mL/mL), and methyl salicylate had significant repellent effects on the brown planthopper. D-limonene, β-caryophyllene, 2-heptanone at high concentration, 2-ethyl-benzenamine, and 2,6,10,14-tetramethyl-pentadecane showed significant attractant effects on the brown planthopper. The results of this study are of significance for the development of repellents or attractants for the green control and integrated pest management of the brown planthopper.

Key words: brown planthopper, rice volatiles, functional effects, screening and identification, selection response

中图分类号:

S476

王兴云, 张新强, 李菁, 黄小玲, 张元臣, 张坤朋, 王景顺. 对褐飞虱有功能效应的水稻挥发物活性组分的筛选与鉴定[J]. 中国生物防治学报, 2023, 39(6): 1474-1481.

WANG Xingyun, ZHANG Xinqiang, LI Jing, HUANG Xiaoling, ZHANG Yuanchen, ZHANG Kunpeng, WANG Jingshun. Screening and Identification of Active Components of Rice Volatiles with Functional Effects on Brown Planthopper, Nilaparvata lugens (Stål)[J]. Chinese Journal of Biological Control, 2023, 39(6): 1474-1481.

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对褐飞虱有功能效应的水稻挥发物活性组分的筛选与鉴定

Screening and Identification of Active Components of Rice Volatiles with Functional Effects on Brown Planthopper, Nilaparvata lugens (Stål)

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