苏云金芽胞杆菌Bt64紫外线照射及其对稻螟蛉的致病力

doi:10.16409/j.cnki.2095-039x.2024.03.006

摘要/Abstract

摘要： 为探索苏云金芽胞杆菌Bt64紫外线照射后菌株抗紫外线能力及对稻螟蛉幼虫的致病力变化，本研究在对苏云金芽胞杆菌菌株Bt64进行紫外线照射的基础上，揭示了不同紫外线照射处理对菌株生长发育的影响和抗紫外线能力变化，明确了紫外线处理后菌株Bt64对稻螟蛉幼虫的毒力变化及室外防治效果。研究结果表明，菌株Bt64不同时长紫外线连续照射处理的菌落形成单位和芽胞产量均高于直接照射，而芽胞萌发抑制率低于直接照射，随照射时长增加差异显著。照射时长30 min和45 min条件下，菌株经连续照射后复壮和连续照射后复壮再照射对稻螟蛉幼虫的致病力和防治效果较原菌株增强，显著高于同时长的直接照射菌株致病力。紫外线连续照射后能提高菌株Bt64抗紫外线能力、杀虫活性和室外防治效果，为开发出具有杀虫活性持久和耐紫外线的Bt生物农药提供了依据。

关键词: 苏云金芽胞杆菌, 紫外线, 稻螟蛉, 菌株生长发育, 致病力

Abstract: To explore the ultraviolet resistance and the pathogenicity changes on Naranga aenescens of Bacillus thuringiensis Bt64 induced by ultraviolet irradiation, this study revealed the effects of different ultraviolet irradiation treatments on the growth and development of B. thuringiensis Bt64 and the change in UV resistance. The toxicity changes and outdoor control effects of Bt64 strain on N. aenescens larvae after ultraviolet treatment were also clarified. The results showed that the colony forming units and spore yield of continuous UV irradiation at different times were all higher than those of direct UV irradiation. The inhibition rate of spore germination was lower than that of direct UV irradiation in different time, and the difference was significant with the increase of irradiation duration. Under the conditions of 30 minutes and 45 minutes irradiation, the pathogenicity and control effect of the rejuvenated strain after continuous UV irradiation and the rejuvenated strain were irradiated again after continuous UV irradiation on the N. aenescens larvae were enhanced, which was significantly higher than that of direct UV irradiation treatment at the same time. The result shows that UV resistant mutant can be selected by continuous UV irradiation, which can enhance the UV resistance, insecticidal activity and outdoor control effect of Bt64 strain, and provide a basis for the development of new biopesticides with high insecticidal activity and persistence.

Key words: Bacillus thuringiensis, ultraviolet, Naranga aenescens, strain growth and development, pathogenicity

中图分类号:

S476.1

马世炎, 韩宇彤, 刘晓丽, 张鑫鑫, 于洪春. 苏云金芽胞杆菌Bt64紫外线照射及其对稻螟蛉的致病力[J]. 中国生物防治学报, 2024, 40(6): 1275-1284.

MA Shiyan, HAN Yutong, LIU Xiaoli, ZHANG Xinxin, YU Hongchun. Ultraviolet Irradiation of Bacillus thuringiensis Bt64 and Its Pathogenicity to Naranga aenescens[J]. Chinese Journal of Biological Control, 2024, 40(6): 1275-1284.

参考文献

[1] Cho J R, Park H H, Seo B Y, et al. Identification and application of the sex pheromones of the rice green caterpillar Naranga aenescens in Korea[J]. Journal of Asia-pacific Entomology, 2014, 17(2): 191-197.
[2] Mahmoud F D, Aziz K P, Faramarz A, et al. Field biology of the green semi-looper, Naranga aenescens Moore (Lepidoptera: Noctuidae) and efficiency determination of Beauveria bassiana isolates[J]. Soaj of Entomological Studies, 2012, 1: 68-80.
[3] Zibaee A, Mahmoud F D. Purification and characterization of a digestive lipase in rice green semi-looper, Naranga aenescens Moore (Lepidoptera: Noctuidae)[J]. SOAJ of Entomological Studies, 2012, 1: 38-54.
[4] 李慧, 刘颖, 蒋毅. 几种生物农药对稻螟蛉的田间防效[J]. 中国植保导刊, 2020, 40(12): 78-79.
[5] Lucero J M, Wilson B E. Regional expansion and chemical control for invasive apple snails (Pomacea maculata) in Louisiana rice and crawfish production systems[J]. Crop Protection, 2023, 164: 106127.
[6] 魏中华, 李鹤鹏. 不同药剂对水田稻螟蛉的防治效果评价[J]. 黑龙江农业科学, 2020(1): 78-80.
[7] Gama Z P, Nakagoshi N, Suharjono, et al. Toxicity studies for indigenous Bacillus thuringiensis isolates from Malang city, East Java on Aedes aegypti larvae[J]. Asian Pacific Journal of Tropical Biomedicine, 2013, 3(2): 111-117.
[8] 王倩倩, 迟德富. 苏云金芽孢杆菌鲇泽亚种和中华甲虫蒲螨对冷杉梢斑螟的杀虫作用[J]. 东北林业大学学报, 2023, 51(7): 146-154.
[9] 于洪春, 王紫君, 任中杰, 等. 白僵菌对二化螟致病力及与Bt混用协同作用研究[J]. 东北农业大学学报, 2023, 54(2): 17-25.
[10] 于洪春, 任中杰, 王紫君, 等. 苏云金芽孢杆菌对二化螟致病力及与助剂协同作用研究[J]. 东北农业大学学报, 2023, 54(1): 12-20, 44.
[11] Bai C, Vick B A. Fermentation of a new Bacillus Thuringiensis strain[J]. Helia, 2001, 24(35): 47-54.
[12] 李超峰. 苏云金芽孢杆菌杀虫活性的增效机制研究进展[J]. 生物技术进展, 2018, 8(1): 14-20.
[13] 翟兴礼. 紫外线对4个Bt亚种活性的影响[J]. 河南农业科学, 2009(7): 82-84.
[14] 王文军, 钱传范, 申继忠, 等. 紫外线对Bt伴孢晶体的损伤和腐植酸的保护作用[J]. 植物保护学报, 2001, 28(1): 49-54.
[15] Saxena D, Eitan B D, Manasherob R, et al. A UV Tolerant mutant of Bacillus thuringiensis subsp. kurstaki producing melanin[J]. Current Microbiology, 2002, 44(1): 25-30.
[16] Zhang J T, Yan J P, Zheng D S, et al. Expression of mel gene improves the UV resistance of Bacillus thuringiensis[J]. Journal of Applied Microbiology, 2008, 105: 151-157.
[17] Margulies L, Rozen H, Cohen E. Photostabilization of a nitromethylene heterocycle insecticide on the surface of montmorillonite[J]. Clays and Clay Minerals, 1988, 36(2): 159-164.
[18] Zhang L L, Zhang X J, Batool K, et al. Comparison and mechanism of the UV-resistant mosquitocidal Bt mutant LLP29-M19[J]. Journal of Medical Entomology, 2018, 55(1): 210-216.
[19] 刘世程, 李磊, 张雪艳, 等. 贝莱斯芽胞杆菌ZF145的诱变及抑菌防病效果研究[J]. 农业生物技术学报, 2022, 30(12): 2417-2424.
[20] Ghribi D, Zouari N, Jaoua S. Improvement of bioinsecticides production through mutagenesis of Bacillus thuringenesis by u.v. and nitrous acid affecting metabolic pathways and/or delta-endotoxin synthesis[J]. Journal of Applied Microbiology, 2004, 97: 336-346.
[21] 于洪春, 王煜, 孙苓芙, 等. 苏云金芽孢杆菌对黏虫致病力及生长发育的影响[J]. 东北农业大学学报, 2021, 52(8): 21-28.
[22] Cutler T D, Zimmerman J J. Ultraviolet irradiation and the mechanisms underlying its inactivation of infectious agents[J]. Animal Health Research Reviews, 2011, 12(1): 15-23.
[23] 黎媛, 陆路, 方呈祥. SOD对紫外辐射受损的苏云金芽孢杆菌作用的研究[J]. 河南农业科学, 2003(2): 20-23.
[24] Cohen E, Rozen H, Joseph T, et al. Photoprotection of Bacillus thuringiensis kurstaki from ultraviolet irradiation[J]. Journal of Invertebrate Pathology, 1991, 57(3): 343-351.
[25] Aronson A I, Tyrell D J, Fitz-James P C, et al. Relationship of the syntheses of spore coat protein and parasporal crystal protein in Bacillus thuringiensis[J]. Journal of Bacteriology, 1982, 151(1): 399-410.
[26] Patel K, Wyman J, Patel K, et al. A mutant of Bacillus thuringiensis producing a dark-brown pigment with increased UV resistance and insecticidal activity[J]. Journal of Invertebrate Pathology, 1996, 67(2): 120-124.