球孢白僵菌和莱氏绿僵菌浸种对玉米抗虫性及叶片防御酶活性的影响

doi:10.16409/j.cnki.2095-039x.2025.01.002

摘要/Abstract

摘要： 为明确球孢白僵菌Beauveria bassiana Bb20091317和莱氏绿僵菌Mtarhizium rileyi MrCDTLJ1浸种对玉米抗虫性及叶片防御酶活性的影响，本试验采用盆栽试验，检测浸种植株叶片对亚洲玉米螟和草地贪夜蛾的抗性以及叠加草地贪夜蛾为害0、2、12、24和48 h后叶片中苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、超氧化物歧化酶（SOD）和过氧化物酶（POD）的活性。结果显示Bb20091317浸种可显著增强草地贪夜蛾为害诱导前后玉米叶片中POD、PPO和SOD活性。MrCDTLJ1浸种可显著增加草地贪夜蛾为害诱导前后玉米叶片中POD和SOD活性，但对SOD和PAL活性无显著诱导。取食Bb20091317、MrCDTLJ1浸种玉米叶片的亚洲玉米螟幼虫体重显著低于取食未浸种玉米叶片的幼虫体重。而取食Bb20091317、MrCDTLJ1浸种叠加草地贪夜蛾为害诱导24 h玉米叶片的草地贪夜蛾幼虫体重与取食未经虫害诱导玉米叶片的幼虫体重相比虽显著降低，但与草地贪夜蛾为害诱导24 h的未浸种玉米叶片饲喂的幼虫相比无显著差异。以上结果表明，Bb20091317和MrCDTLJ1浸种能诱导玉米产生系统防御反应，在草地贪夜蛾为害诱导后会产生强烈的酶活响应，同时参与调控玉米对亚洲玉米螟和草地贪夜蛾的抗性。

关键词: 球孢白僵菌, 莱氏绿僵菌, 玉米, 亚洲玉米螟, 草地贪夜蛾, 防御酶

Abstract: This study aim is to delve into the impact of seed treatment using entomopathogenic fungi Beauveria bassiana Bb20091317 and Metarhizium rileyi MrCDTLJ1 on insect resistance and the activity of defense enzymes in maize. Employing a pot experiment setup, the investigation aimed to evaluate the resistance of maize leaves which seeds soaked with B. bassiana Bb20091317 and M. rileyi MrCDTLJ1, respectively, against Ostrinia furnacalis and Spodoptera frugiperda, as well as the activity of levels of key enzymes such as phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), superoxide dismutase (SOD), and peroxidase (POD) in S. frugiperda after injured maize leaves over various time intervals (0, 2, 12, 24, and 48 hours). The findings revealed that treated seeds with Bb20091317 significantly blostered the activities of POD, PPO, and SOD in maize leaves, both before and after the induction of S. frugiperda damage. Similarly, soaked seeds with MrCDTLJ1 significantly elevated POD and SOD activities in maize leaves before and after S. frugiperda damage, but did not significantly induce SOD and PAL activities. Notably, the weight of O. furnacalis larvae feeding on the leaves which seeds treated with Bb20091317 and MrCDTLJ1 was significantly lower compared to those feeding on non-soaked maize leaves. Additionally, the weight of S. frugiperda larvae induced by Bb20091317 and MrCDTLJ1-treated seeds, coupled with 24 hours of leaf damage, was significantly lower than larvae from untreated maize leaves subjected to S. frugiperda damage. However, there was no significant difference in the weight of larvae from S. frugiperda induced maize leaves for 24 hours compared to control leaves. In essence, the seed treatments with B. bassiana and M. rileyi stimulated systemic defense responses in maize, as evidenced by heightened enzyme activation following S. frugiperda herbivory, consequently enhancing resistance against O. furnacalis and S. frugiperda.

Key words: Beauveria bassiana, Metarhizium rileyi, Zea mays, Ostrinia furnacalis, Spodoptera frugiperda, defense enzymes

中图分类号:

S476.1

展昭凤, 苟雪莲, 王振营, 张永军, 王秀平, 郭井菲. 球孢白僵菌和莱氏绿僵菌浸种对玉米抗虫性及叶片防御酶活性的影响[J]. 中国生物防治学报, 2025, 41(1): 54-62.

ZHAN Zhaofeng, GOU Xuelian, WANG Zhenying, ZHANG Yongjun, WANG Xiuping, GUO Jingfei. Effects of Seed Soaking with Beauveria bassiana and Metarhizium rileyi on Defense Enzyme Activities and Insect Resistance in Maize Leaves[J]. Chinese Journal of Biological Control, 2025, 41(1): 54-62.

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