稻纵卷叶螟卵沉积诱导水稻排斥后续成虫产卵的生态机制

doi:10.16409/j.cnki.2095-039x.2024.07.023

摘要/Abstract

摘要： 产卵行为是昆虫完成种群延续的关键环节，而定位适宜产卵的寄主植物对昆虫的繁衍至关重要。昆虫在寄主植物上产卵可触发植物的抗虫防御机制，该机制不仅可以直接阻碍昆虫卵的发育，还可以为防御后续幼虫的攻击做好准备。本文探究了稻纵卷叶螟卵沉积诱导稻株对稻纵卷叶螟成虫产卵选择的影响，以及其对卵诱导水稻挥发物的响应。温室试验显示，卵沉积4 d的稻株无论是否去卵对稻纵卷叶螟雌成虫均具有显著的排斥作用。而且相对于健康稻株，产在卵沉积4 d稻株上的稻纵卷叶螟卵孵化期延长，孵化率显著降低。采用GC-MS分析了健康稻株和卵沉积4 d稻株挥发物的变化，发现两组处理稻株挥发物的组分并无显著差异，均鉴定到22种化合物，但有6种挥发物的释放量在两处理稻株间具有显著性差异，除苯乙烯外，其它5种挥发物（癸醛、十二烷、十三烷、十四烷和壬醛）在卵沉积诱导下释放量显著下降。挥发物功能测定试验显示，释放量最大的十四烷对稻纵卷叶螟产卵具有极显著的吸引作用，而癸醛对其有显著的排斥作用，其余4种挥发物对稻纵卷叶螟产卵行为无显著影响。这说明稻纵卷叶螟偏爱在健康稻株上产卵可能源于卵沉积稻株十四烷释放量的降低带来的生态效应，也可能源于包括癸醛在内的多种水稻挥发物对昆虫行为的综合调控作用。本研究结果有利于深入理解稻纵卷叶螟的寄主选择机制，并为制定稻纵卷叶螟的生态防控新策略提供参考。

关键词: 稻纵卷叶螟, 卵沉积, 水稻挥发物, 产卵选择

Abstract: Oviposition behavior is a critical process for insects to ensure the continuation of their population, and location of the suitable host plants is of key important for reproductive success of insects. Oviposition on host plants can activate the plant's defense mechanisms against insects. This defense mechanism can not only directly impede the development of insect eggs, but also make the plant to prepare for defense against subsequent larval attacks. In this study, we explored that the impact of Cnaphalocrocis medinalis (RLF) egg-deposited rice plants on oviposition preference of the subsequent adults, and their response to volatile compounds induced by egg deposition. The greenhouse experiments revealed that rice plants with egg deposition for 4 days exhibited a significant repellent effect on RLF females, regardless of whether the eggs were removed or not. Moreover, compared to the healthy plants, RLF eggs laid on rice plants that had undergone 4 days of egg deposition displayed a prolonged hatching periods and reduced hatching rates. GC-MS analysis showed that healthy rice plants and plants with eggs deposition for 4 days released the same kinds of 22 volatile compounds. However, there were 6 volatiles showing significantly different in amount between the two treated rice plants. Among the 6 compounds, except for styrene, the release amounts of 5 other volatiles (decanal, dodecane, tridecane, tetradecane, and nonanal) significantly decreased by the plants with egg deposition. The function of the volatile compounds was tested and the results indicated that tetradecane with the highest release rate exhibited a significant attraction to RLF female oviposition, while decanal displayed a marked repellent effect on RLF females. The other 4 volatiles showed no significant effect on the oviposition behavior of the pest. This may suggest that the RLF females prefer to lay eggs on healthy rice plants may be due to the ecological effects of reduced tetradecane release from egg-deposited rice plants, or due to the integrated regulation of RLF female behavior by multiple rice volatiles, including decanal. These findings enhance our understanding of the host plant selection mechanism of RLF and provide valuable insights for the development of ecological management strategies for control of this pest.

Key words: Cnaphalocrocis medinalis, eggs deposition, rice volatiles, oviposition preference

中图分类号:

S476

邢靖远, 田志强, 姚诚诚, 胡晓云, 褚栋, 李云河. 稻纵卷叶螟卵沉积诱导水稻排斥后续成虫产卵的生态机制[J]. 中国生物防治学报, 2025, 41(2): 299-308.

XING Jingyuan, TIAN Zhiqiang, YAO Chengcheng, HU Xiaoyun, CHU Dong, LI Yunhe. Ecological Mechanism Underlying the Repellence of Cnaphalocrocis medinalis Egg-deposited Rice Plant on the Subsequent Adult Oviposition[J]. Chinese Journal of Biological Control, 2025, 41(2): 299-308.

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